Estrogen Receptor 1 (ESR1) Mouse Monoclonal Antibody [Clone ID: 5D4B1]

CAT#: AM06176SU-N

Estrogen Receptor 1 (ESR1) mouse monoclonal antibody, clone 5D4B1, Ascites

Product Images

Western blot analysis using ER antibody Cat.-No AM06176SU-N truncated ER recombinant protein (Lane 1) MCF-7 cell lysates (Lane 2).

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  Western blot analysis using ER antibody Cat.-No AM06176SU-N against HEK293T cells transfected with the pCMV6-ENTRY Control (Lane 1) and pCMV6-ENTRY ER cDNA (Lane 2).

Specifications

Product Data

• Clone Name: 5D4B1
• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Bloting: 1/500 - 1/2000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of ER expressed in E. Coli.
• Specificity: Recognizes ER (Estrogen Receptor 1).
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: estrogen receptor 1
• Database Link:
  Entrez Gene 2099 Human
  UniProt ID P03372
• Background: ER (Estrogen Receptor 1) a member of the steroid receptor superfamily, contains highly conserved DNA binding (DBD) and ligand binding domains (LBD). Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ER regulates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation provides an important mechanism to regulate ER activity. ER is phosphorylated on multiple sites. Serines 104, 106, 118 and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serines plays an important role in regulating ER activity. Ser118 may be the substrate of the transcription regulatory kinase cdK7. Ser167 may be phosphorylated by p90RSK and Akt. Phosphorylation of Ser167 may confer tamoxifen resistance in breast cancer patients.
• Synonyms: ER alpha, Estradiol receptor, ESR1, ESR, NR3A1