c Kit (KIT) Mouse Monoclonal Antibody [Clone ID: 8D7]

CAT#: AM06162SU-N

c Kit (KIT) mouse monoclonal antibody, clone 8D7, Ascites

Product Images

Western blot analysis using C-kit antibody Cat.-No AM06162SU-N against truncated C-kit recombinant protein.

• 

  Immunohistochemical analysis of paraffin-embedded maligant mesenchymoma tissues, showing cytoplasmic localization using C-kit antibody Cat.-No AM06162SU-N with DAB staining.

Specifications

Product Data

• Clone Name: 8D7
• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1/500 - 1/2000.
  Immunohistochemistry on Paraffin Sections: 1/200 - 1/1000.
  ELISA: 1/10000.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of C-kit expressed in E. Coli.
• Specificity: Recognizes C-kit (CD117, 145kDa).
• Formulation: State: Ascites
  State: Ascitic fluid containing 0.03% Sodium Azide.
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: 145 kDa
• Gene Name: KIT proto-oncogene receptor tyrosine kinase
• Database Link:
  Entrez Gene 3815 Human
  UniProt ID P10721
• Background: C-kit (CD117, 145kDa) functions as a tyrosine kinase receptor which becomes activated upon binding of its ligand SCF (stem-cell factor), the C-kit gene encodes the human homolog of the proto-oncogene c-kit. which was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor). Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.
• Synonyms: SCFR, KIT