ORF45 ORF45 Mouse Monoclonal Antibody [Clone ID: 2D4A5]

CAT#: AM06086SU-N

ORF45 ORF45 mouse monoclonal antibody, clone 2D4A5, Ascites

Product Images

Western blot analysis using KSHV ORF45 antibody AM06086SU-N against KSHV ORF45 recombinant protein.

• 

  Immunocytochemistry analysis of TPA induced BCBL-1 cells (A) and uninduced BCBL-1 cells (B) using KSHV ORF45 antibody AM06086SU-N with AEC staining.

Specifications

Product Data

• Clone Name: 2D4A5
• Applications: IF, WB
• Recommended Dilution: ELISA: 1/10000.
  Western Blot: 1/500 - 1/2000.
  Immunofluorescence: 1/200 - 1/1000.
  Immunohistochemistry: 1/200 - 1/1000.
  
• Reactivities: Kaposi-Sarkom-Herpesvirus
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of KSHV ORF45 expressed in E. Coli.
• Specificity: This antibody recognizes KSHV ORF45
• Formulation: State: Ascites
  State: Ascitic fluid
  Preservative: 0.03% Sodium Azide
• Conjugation: Unconjugated
• Storage:

  Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Database Link:
  UniProt ID Q2HR88
• Background: Kaposi's sarcoma-associated herpesvirus (KSHV) belongs to the gamma-(2)-herpesvirus subfamily and has been closely linked to the Kaposi’s sarcoma, primary effusion lymphoma (PEL) and multicentric Castleman’s disease. The genome of KSHV is 165-170 kb and contains at least 88 open reading frames. ORF45 protein encoded by an immediate-early gene in the KSHV genome is characterized as a phosphorylated protein, and it is localized in the cytoplasm of infected cells. Studies have shown that ORF45 Protein interacted with cellular IRF-7 and blocked virus-mediated phosphorylation and nuclear translocation of IRF-7. In consequence, ORF45 efficiently inhibited virus-induced production of type I IFN. Zhu et al (2003) reported that the ORF45 Protein was associated with purified virions
• Synonyms: KSHV, Human gammaherpesvirus 8, HHV-8