Siglec1 Rat Monoclonal Antibody [Clone ID: 3D6.112]

CAT#: AM05902FC-T

Siglec1 rat monoclonal antibody, clone 3D6.112, FITC

Product Images

Mouse Peritoneal Macrophages stained with Rat anti Mouse CD169 Antibody -FITC (Clone 3D6.112)

Specifications

Product Data

• Clone Name: 3D6.112
• Applications: FC
• Recommended Dilution: Flow Cytometry: Neat-1/5 (The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors).
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Purified Sialoadhesin.
  Spleen cells from an immunised AO rat were fused with the cells of the Y3 rat myeloma cell line.
• Specificity: This antibody recognizes Mouse CD169, a sialic acid binding site of sialoadhesin.
• Formulation: PBS
  Label: FITC
  State: Liquid purified IgG fraction from Tissue Culture Supernatant
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Should this product contain a precipitate we recommend microcentrifugation before use.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: sialic acid binding Ig-like lectin 1, sialoadhesin
• Database Link:
  Entrez Gene 20612 Mouse
  UniProt ID Q62230
• Background: Two families of mammalian lectin like adhesion molecules have been shown to bind glycoconjugate ligands in a sialic acid dependent manner: the selectins and the sialoadhesins. The sialoadhesin family has 4 members: CD22, a B cell specific marker; myelin associated glycoprotein (MAG), which is expressed on oligodendrocytes and Schwann cells; CD33, a myeloid differentiation antigen; and sialoadhesin, which is expressed only by a subpopulation of tissue macrophages. Involved in cell-cell interactions, sialoadhesin is structurally related to the 3 other listed members of the sialoadhesin family. CD169 is a sialic acid binding site of sialoadhesin. CD169 is a macrophage receptor expressed on stromal macrophages in many tissues, particularly found in lymph nodes, bone marrow and spleen.
• Synonyms: Sialoadhesin, Siglec-1