Macrophages Mouse Monoclonal Antibody [Clone ID: BA4D5]

CAT#: AM05890FC-N

Macrophages mouse monoclonal antibody, clone BA4D5, FITC

Specifications

Product Data

• Clone Name: BA4D5
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat antibody to label 1x10⁶ cells in 100 µl.
  Membrane permeabilisation is required.
• Reactivities: Porcine
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Porcine alveolar macrophages. Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 mouse myeloma cell line.
• Specificity: This antibody recognises Porcine cells of the monocyte/macrophage lineage. Expression of the antigen is increased with maturation, with higher expression on peritoneal and alveolar macrophages.
  Some expression has also been observed on peripheral blood lymphocytes.
  The antigen recognised by this antibody Cat.-No AM05890FC-N (clone BA4D5) has a broad tissue distribution. Clone BA4D5 stains macrophages in a range of tissues, including the thymus, spleen periarteriolar lymphoid sheath (PALS), spleen red pulp and the Peyer's patches.
  It is believed that clone BA4D5 may be specific for porcine CD68, although the protein recognised by this antibody has not yet been fully characterised. The protein is expressed on the cell surface, although it is most abundantly expressed in the cytoplasm.
• Formulation: PBS, pH 7.4
  Label: FITC
  State: Liquid purified IgG fraction
  Stabilizer: 1% BSA
  Preservative: 0.09% Sodium Azide
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G
• Conjugation: FITC
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  This product is photosensitive and should be protected from light.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Synonyms: Macrophage marker