CD8A Rat Monoclonal Antibody [Clone ID: YTC182.20]

CAT#: AM05690PU-N

CD8A rat monoclonal antibody, clone YTC182.20, Purified

Specifications

Product Data

• Clone Name: YTC182.20
• Applications: FC, IHC
• Recommended Dilution: Immunohistochemistry on frozen sections.
  Flow Cytometry: 1/100 - 1/200.
• Reactivities: Human, Monkey
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Mouse L cells transfected with human CD8 gene
• Specificity: This antibody recognises the CD8 cell surface antigen, a 30/32kD glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells.
• Formulation: Phosphate buffered saline pH7.4 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD8a molecule
• Database Link:
  Entrez Gene 925 Human
  UniProt ID P01732
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL