IRF3 Mouse Monoclonal Antibody [Clone ID: 3F10]

CAT#: AM05643PU-N

IRF3 mouse monoclonal antibody, clone 3F10, Purified

Product Images

Western blot analysis on HeLa cells: Cell lysates of HeLa (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human IRF-3 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Immunofluorescence of human HeLa cells stained with IRF-3 (1:500) with Texas-Red and Hoechst 33342 (Blue) for nucleus staining.

Specifications

Product Data

• Clone Name: 3F10
• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA.
  Western blot (1:1000 - 1:2000), recommended starting dilution is 1:1000.
  Immunoprecipitation.
  Immunoflourescence.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Recombinant human IRF3 (108-166 aa) purified from E.coli
• Specificity: The antibody recognizes human interferon regulatory factor 3 (IRF3), a 55 kDa constitutively expressed member of the IRF family.
  Other species not tested.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: interferon regulatory factor 3
• Database Link:
  Entrez Gene 3661 Human
  UniProt ID Q14653
• Background: Members of the Interferon regulatory factor (IRF) family regulate gene expression critical to immune response, hemopoiesis, and proliferation. IRF-3 is distinct from other family members. Its transcriptional activity is regulated solely by posttranslational modifications. It plays a crucial role in activation of innate immunity and inflammation in response to viral infection.
  IRF3 accumulates in an inactive form in the cytoplasm and then translocates to the nucleus as a homodimer, following stimuli-induced phosphorylation of specific serine/threonine residues. Association of the homodimer with p300/CBP (CREBB binding protein) forms dsRNA-activated factor 1 (DRAF1), which then activates the transcription of interferon alpha and beta, along with other interferon-induced genes.
• Synonyms: Interferon regulatory factor 3, IRF-3