CD8A Mouse Monoclonal Antibody [Clone ID: 4B11]

CAT#: AM05530SU-N

CD8A mouse monoclonal antibody, clone 4B11

Specifications

Product Data

• Clone Name: 4B11
• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on Frozen Sections: 1/50.
  Immunohistochemistry on Paraffin Sections: 1/50.
  This product requires antigen retrieval using heat treatment prior to staining of paraffin sections; 1mM EDTA pH8.0 is recommended for this purpose.
  Western Blot: 1/25.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: Synthetic peptide derived from CD8 alpha chain sequence.
• Specificity: This antibody recognizes the Human CD8 cell surface antigen, a 32kD glycoprotein expressed by the cytotoxic/suppressor subset of T-cells and weakly by NK cells.
• Formulation: State: Lyophilised Tissue Culture Supernatant
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Restore with 1 ml distilled water.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  
• Stability: Shelf life: one year from despatch.
• Gene Name: CD8a molecule
• Database Link:
  Entrez Gene 925 Human
  UniProt ID P01732
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
• Synonyms: CD8 alpha chain, CD8A, MAL