Caspase-7 (CASP7) Mouse Monoclonal Antibody [Clone ID: MCH3-5]

CAT#: AM05296PU-N

Caspase-7 (CASP7) mouse monoclonal antibody, clone MCH3-5, Purified

Product Images

Figure 1. Western blot analysis using caspase-7 antibody on MCF-7 cells treated with thapsigargin for 48 hours which are negative (-) and positive (+) for caspase-3.

Specifications

Product Data

• Clone Name: MCH3-5
• Applications: WB
• Recommended Dilution: Western Blot: Detects proenzyme form of human Caspase-7.
  Positive Control: MCF-7 cell line.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with recombinant human caspase-7 protein and mouse myeloma cells.
• Specificity: Reacts with Cysteine-requiring Aspartate Protease-7.
• Formulation: PBS containing 0.08% Sodium Azide as preservative.
  State: Purified
  State: Liquid (sterile filtered) purified IgG fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: caspase 7
• Database Link:
  Entrez Gene 840 Human
  UniProt ID P55210
• Background: Caspases are key effectors of programmed cell death. Caspase-7 along with caspase 3 and 6 form the group of effector caspases that are responsible for the cleavage of multiple substrates including the cytokeratins, PARP, alpha fodrin, NuMA and others. Caspase-7 is a 303 amino acid protein with high similarity to caspase-3. Caspase-7 occurs in three varient forms. Granzyme B activates pro-caspase-7 to a form which can cleave poly(ADP-ribose) polymerase (PARP) to its signature fragment of ~85 kDa. Also, in vivo caspase-7 appears to be a better substrate for granzyme B than caspase-3. Pro-caspase-7 has been shown to exist as dimers or high order oligomers. Caspase-7 may be an important intracellular effector of granzyme B-mediated apoptosis and cytotoxic T-lymophocyte-induced cell killing in vivo.
• Synonyms: CASP-7, CASP7, MCH3, CMH-1