6xHistidine Epitope Tag (HHHHHH) Mouse Monoclonal Antibody [Clone ID: IPA2C5.1]

CAT#: AM05288PU-N

6xHistidine Epitope Tag (HHHHHH) mouse monoclonal antibody, clone IPA2C5.1, Purified

Product Images

Figure 1. Western blot of His tagged protein (vector pET 30C) using AM05288PU-N antibody. Lane 1: Whole cellular extract of BL21 (DE3) pJT4pTT9 bacteria carrying gene encoding for His-Rel E protein (uninduced). Lane 2: Induced. Lane 3: Induced (1/2 dilutio

Specifications

Product Data

• Clone Name: IPA2C5.1
• Applications: WB
• Recommended Dilution: Western Blot.
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Hybridoma produced by the fusion of splenocytes from mice immunized with His-His-His-His-His-His synthetic peptide and mouse myeloma cells.
• Specificity: This antibody is effective in detecting proteins expressed using the following vector systems, pET 28, 30 and 32 as well as Invitrogen's pIZ.
  Specific for 6x-His tags only and for His tags no longer than 6 histidines.
• Formulation: PBS containing 0.08% Sodium Azide as preservative.
  State: Purified
  State: Liquid (sterile filtered) purified IgG fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Background: 6x-His tags are a type of tag for expressed proteins. This tag is made up of 6 histidine residues attached to either the N- or C-terminal of a protein. Proteins expressed with this type of tag are then traditionally purified using a divalent metal ion column. However, using an antibody specific to this 6x-His tag, the protein can be analyzed for by Western blot or immunofluorescence techniques, thus eliminating the need for a protein-specific antibody. 6x-His tags are particularly effective for the purification of protein expressed in E. coli systems.