Adenovirus Type 2 E1A Mouse Monoclonal Antibody [Clone ID: M73]

CAT#: AM05218PU-N

Adenovirus Type 2 E1A mouse monoclonal antibody, clone M73, Purified

Specifications

Product Data

• Clone Name: M73
• Applications: IF, IP
• Recommended Dilution: Immunoprecipitation (1-2 ug/ml): Adenovirus 2E1A recognizes adenovirus 2 and 5 E1A proteins, 3 bands at 30-50kDa, as well as, E1A associated proteins.
  Immunofluorescence: 2-5 ug/ml.
  293 cells can be used as positive control and HS27 cells as negative control.
  Positive Control: 293 cells are human embryonic kidney cells that have been transformed with the adenovirus genes E1A and E1B, which are required for adenovirus propagation. This cell line can be used as a positive control for this antibody.
• Reactivities: Adeno-associated Virus 2, Adeno-associated Virus 5
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Full length recombinant adenovirus 2 E1A protein.
• Specificity: Adenovirus-infected cells and tissue.
• Formulation: PBS containing 0.08% Sodium Azide as preservative.
  State: Purified
  State: Liquid (sterile filtered) purified IgG fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Database Link:
  UniProt ID P03254
• Background: The early region (E1) of the adenovirus genome, responsible for transforming activity, is localized within the left most 11% of the viral genome and consists of two transcriptional units E1A and E1B. E1A is sufficient for partial transformation and immortalization of primary Cells. E1A gene products are necessary for normal levels of transcription of the other early regions of the adenovirus genome during productive infection and are able to either activate or repress the transcription of specific cellular genes. E1A forms specific complexes with cellular proteins including p105 causing inhibition of the cell cycle inducing arresting function of p105.
• Synonyms: Adenovirus Early Region 1