PPP1R14A Mouse Monoclonal Antibody [Clone ID: 4H10]

CAT#: AM03185PU-S

PPP1R14A mouse monoclonal antibody, clone 4H10, Aff - Purified

Product Images

The Cell lysates of A549 (30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1R14A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

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  Western blot analysis The Cell lysates of A549 (30 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPP1R14A (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Specifications

Product Data

• Clone Name: 4H10
• Applications: ELISA, WB
• Recommended Dilution: ELISA.
  Western blot: 1:250 ~ 1:1000 (1:500 as startin dilution).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Recombinant human PPP1R14A (1-147aa) purified from E. coli
• Specificity: This antibody detects PPP1R14A.
• Formulation: PBS, pH 7.4 containing 0.02% Sodium Azide and 10% Glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein-G affinity chromatography
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: protein phosphatase 1 regulatory inhibitor subunit 14A
• Database Link:
  Entrez Gene 94274 Human
  UniProt ID Q96A00
• Background: PPP1R14A (Protein phosphatase 1 regulatory subunit 14A) is a phosphorylation-dependent inhibitory protein for smooth muscle myosin phosphate. Myosin phosphatase can reverse MYL (myosin light chain) phosphorylation to induce a state of relaxation. However, during agonist-induced contraction at constant Ca2+ concurrent inhibition of myosin phosphatase leads to increases in MYL phosphorylation and tension. These calcium-independent increases in myosin phosphorylation and tension are termed calcium sensitization.
• Synonyms: Protein phosphatase 1 regulatory subunit 14A, CPI-17, CPI17, PPP1INL