PSMA (FOLH1) (44-750) Mouse Monoclonal Antibody [Clone ID: GCP-05]

CAT#: AM03114RP-N

PSMA (FOLH1) (44-750) mouse monoclonal antibody, clone GCP-05, PE

Product Images

Surface staining (flow cytometry) of GCPII / PSMA using anti-GCPII (GCP-05) PE on LNCaP cell line.

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  Flow cytometry analysis of GCPII (PSMA) using anti-GCPII (GCP-05) and goat anti-mouse-PE on LNCaP cell line (positive, green) and HeLa cells (negative, blue).

Specifications

Product Data

• Clone Name: GCP-05
• Applications: FC
• Recommended Dilution: Flow Cytometry: 1/200.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Amino acids 44-750 of Human PSMA / FOLH1
• Specificity: This antibody recognizes extracellular domain of Glutamate Carboxypeptidase II (NAALADase, FOLH1, PSMA), an approximately 95-110 kDa transmembrane glycoprotein expressed mainly in tumour neovasculatures, nervous system and jejunum, which is an important prostate tumour marker.
• Formulation: Phosphate buffered saline (PBS)
  Label: PE
  State: Liquid purified Ig fraction
  Preservative: 15 mM Sodium Azide
  Label: Conjugated with R-Phycoerythrin under optimum conditions.
• Concentration: lot specific
• Purification: Size-Exclusion Chromatography
• Conjugation: PE
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: folate hydrolase (prostate-specific membrane antigen) 1
• Database Link:
  Entrez Gene 2346 Human
  UniProt ID Q04609
• Background: Glutamate carboxypeptidase II (GCPII), also known as N-acetyl-alpha-linked acidic dipeptidase I (NAALADase I), folate hydrolase (FOLH1), and prostate-specific membrane antigen (PSMA), is an approximately 95-110 kDa type II transmembrane glycoprotein expressed in various tissues. In nervous system GCPII cleaves abundant N-acetylaspartylglutamate, which is released from neurons in a calcium-dependent manner, to N-acetylaspartate and glutamate. As immoderate glutamate concentration is neurotoxic, GCPII contributes to pathological conditions regarding e.g. Alzheimer´s disease, Huntington´s disease, epilepsy, schizophrenia, stroke or neuropathic pain and appears to be an interesting therapeutic target. In jejunum GCPII hydrolyzes pteroylpoly-gamma-glutamate to folate and glutamate, enabling folate to be absorbed by gastrointestinal tract. GCPII, which is present in a number of tissues at low levels, is overexpressed in neovasculature of most solid tumours and is a target enzyme for diagnosis and treatment of prostate cancer. Normal human prostate express more mRNA coding for a cytosolic GCPII form truncated at the N-terminus (PSM´) than mRNA for membrane-bound GCPII, and this ratio is reversed upon malignant transformation.
• Synonyms: Glutamate carboxypeptidase 2, Folate hydrolase 1, Prostate-specific membrane antigen, FOLH, NAALAD1, PSM, GCP2, NAALAdase

Reference Data

• Protein Families: Druggable Genome, Protease, Transmembrane