HLAG (HLA-G) Mouse Monoclonal Antibody [Clone ID: G233]

CAT#: AM03104PU-N

HLAG (HLA-G) mouse monoclonal antibody, clone G233, Aff - Purified

Product Images

Surface staining of HLA-G in HLA-G transfectants using anti-HLA-G (G233) purified / GAM-APC.

Specifications

Product Data

• Clone Name: G233
• Applications: ELISA, FC, IHC, IP
• Recommended Dilution: Flow Cytometry: Extracellular and intracellular staining; recommended dilution: 1-4 μg/ml.
  Immunoprecipitation.
  ELISA.
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Murine L cells transfected with both human beta2-microglobulin and HLA-G
• Specificity: The antibody G233 recognizes several isoforms of HLA-G expressed in all populations of extravillous trophoblast (cell columns, interstitial trophoblast, endovascular trophoblast, placental bed giant cells). HLA-G belongs to the nonclassical MHC Class I molecules (MHC Class Ib). The antibody G233 has been found not to cross-react with any other MHC Class I antigens (HLA-A, -B, -C, -E, -F).
• Formulation: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A affinity chromatography (> 95% by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: major histocompatibility complex, class I, G
• Database Link:
  Entrez Gene 3135 Human
  UniProt ID P17693
• Background: HLA G belongs to the HLA class I heavy chain paralogues. It is a heterodimer consisting of a heavy chain (with a1, a2, a3 domains) and a light chain (beta 2 microglobulin). Alternate splicing provides both soluble and membrane bound forms of HLA G, with two soluble isoforms and four membrane bound isoforms. HLA G may be part of the fetal protection system and is expressed on fetal derived placental cells.
• Synonyms: HLA-6.0, HLAG, MHC class I antigen G

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, Natural killer cell mediated cytotoxicity, Type I diabetes mellitus, Viral myocarditis