CD5 Mouse Monoclonal Antibody [Clone ID: CRIS1]

CAT#: AM03103PU-N

CD5 mouse monoclonal antibody, clone CRIS1, Aff - Purified

Product Images

Flow Cytometry analysis of human Peripheral Blood Lymphocytes (PBL) stained with CRIS1 antibody (dilution of purified antibody 1 ug/ml); Fig. A:Histogram - gated on lymphocytes, overlay with isotypic control (mouse IgG2a); Fig. B Dot plot.

• 

  Figure 2. Flow Cytometry analysis -Dot plot.

Specifications

Product Data

• Clone Name: CRIS1
• Applications: ELISA, FC, IHC, IP, WB
• Recommended Dilution: Immunoprecipitation.
  Flow cytometry: 1 μg/ml
  Positive Control:
  Peripheral Blood Lymphocytes (PBL)
  Jurkat human leukemia T-cell line.
  HPB human leukemia T-cell line.
  MOLT-4 human leukemia T-cell line.
  Western blot: Laurylmaltoside lysing buffer; non-reducing conditions; 1-2 μg/ml.
  Positive Control:
  Jurkat human leukemia T-cell line.
  HPB human leukemia T-cell line.
  Immunohistochemistry (Frozen sections).
  ELISA: Can be used in the Sandwich ELISA as the detection antibody in pair with the capture antibody MEM-32.
  
  
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: Stimulated human leukocytes.
• Specificity: The antibody CRIS1 reacts with the cell surface glycoprotein CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).
• Formulation: PBS, pH 7.4 with 15 mM sodium azide as preservative.
  State: Aff - Purified
  State: Liquid purified IgG fraction (> 95% pure by SDS-PAGE).
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein-A.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD5 molecule
• Database Link:
  Entrez Gene 921 Human
  UniProt ID P06127
• Background: CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.
  Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
• Synonyms: CD5, LEU1