CD79A (202-216) Mouse Monoclonal Antibody [Clone ID: HM57]

CAT#: AM01322FC-N

CD79A (202-216) mouse monoclonal antibody, clone HM57, FITC

Product Images

Intracellular staining of CD79a with anti-CD79a (HM57) PE (gated on leukemic blast cells) in a patient with childhood B-precursor ALL.

Specifications

Product Data

• Clone Name: HM57
• Applications: FC
• Recommended Dilution: Flow Cytometry analysis of human blood cells: 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension.
  The content of a vial (0.4 ml) is sufficient for 100 tests.
• Reactivities: Bovine, Chicken, Equine, Guinea Pig, Human, Mouse, Opossum, Porcine, Rabbit, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Synthetic peptide corresponding to 202-216 amino acid sequence of Human mb-1.
• Specificity: This antibody interacts with CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.
• Formulation: PBS
  Label: FITC
  State: Liquid purified IgG fraction
  Stabilizer: 0.2% (w/v) BSA (high-grade protease free)
  Preservative: 15 mM sodium azide
  Label: Conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
• Conjugation: FITC
• Storage: Store undiluted at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: CD79a molecule
• Database Link:
  Entrez Gene 973 Human
  UniProt ID P11912
• Background: CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.
• Synonyms: IGA, MB1, B-Cell marker