EMR3 (ADGRE3) Hamster Monoclonal Antibody [Clone ID: 3D7]

CAT#: AM01316PU-S

EMR3 (ADGRE3) hamster monoclonal antibody, clone 3D7, Purified

Specifications

Product Data

• Clone Name: 3D7
• Applications: FC, IP
• Recommended Dilution: Immunoprecipitation.
  Flow Cytometry: 1/50 - 1/200; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
• Reactivities: Human
• Host: Hamster
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: ARHO-EMR3-CD97 (EGF1) transfectants. Spleen cells from immunised Armenian hamster were fused with cells of the mouse SP2/0 myeloma cell line.
• Specificity: This antibody recognises epidermal growth factor (EGF) module-containing mucin-like hormone receptor 3 (EMR3), which is a 56kD member of the EGF-7 transmembrane (TM7) family of adhesion receptors.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified IgG
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: adhesion G protein-coupled receptor E3
• Database Link:
  Entrez Gene 84658 Human
  UniProt ID Q9BY15
• Background: EMR3 is expressed at the cell surface as a heterodimer. The molecule is predominantly expressed on granulocytes, and at lower levels on mature myeloid cells, monocytes and dendritic cells. EMR3 is absent on lymphocytes, haematopoietic stem cells and myeloid progenitors. Studies suggest that the EMR3 molecule is up-regulated during late stages of neutrophil differentiation and is a marker for terminally differentiated cells. The exact functions of EMR3 and its ligands have not yet been determined.