CD88 / C5R1 Rat Monoclonal Antibody [Clone ID: 20/70]

CAT#: AM01270RP-N

CD88 / C5R1 rat monoclonal antibody, clone 20/70, PE

Specifications

Product Data

• Clone Name: 20/70
• Applications: FC
• Recommended Dilution: Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
  The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: RBL-2H3 transfected cells expressing murine C5aR
• Specificity: This antibody recognises CD88.
  Clone 20/70 has been reported to block the binding of the C5a to murine CD88. We recommend the use of AM01270LE-N for this purpose.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide as preservative and 1% BSA as stabilizer.
  Label: PE
  State: Lyophilized purified IgG fraction.
  Label: R. Phycoerythrin (RPE)
• Reconstitution Method: Restore with 1 ml distilled water.
• Concentration: lot specific
• Purification: Affinity Chromatography on Protein G.
• Conjugation: PE
• Storage: Store the antibody undiluted Prior to and Following Reconstitution at 2-8°C.
  DO NOT FREEZE!
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Gene Name: complement component 5a receptor 1
• Database Link:
  Entrez Gene 12273 Mouse
  UniProt ID P30993
• Background: CD88 is a 45kD G-protein coupled cell surface receptor, which is otherwise known as C5aR. The CD88 molecule functions as a receptor for the complement component C5a, which is a potent proinflammatory molecule and a chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes.
• Synonyms: C5a-R, C5aR, C5AR1, Complement Component 5a Receptor 1