CD88 / C5R1 Rat Monoclonal Antibody [Clone ID: 20/70]

CAT#: AM01270PU-S

CD88 / C5R1 rat monoclonal antibody, clone 20/70, Purified

Specifications

Product Data

• Clone Name: 20/70
• Applications: FC
• Recommended Dilution: Flow Cytometry: 1/50 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
• Reactivities: Mouse
• Host: Rat
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: RBL-2H3 transfected cells expressing murine C5aR. Cells from immunised Lou/c were fused with cells of the X63-Ag8.653 myeloma cell line.
• Specificity: This antibody recognises murine CD88.
• Formulation: PBS, pH 7.4 containing 0.09% Sodium Azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: complement component 5a receptor 1
• Database Link:
  Entrez Gene 12273 Mouse
  UniProt ID P30993
• Background: CD88 is a 45kD G-protein coupled cell surface receptor, which is otherwise known as C5aR. The CD88 molecule functions as a receptor for the complement component C5a, which is a potent proinflammatory molecule and a chemoattractant for neutrophils to sites of infection. In mouse, CD88 is expressed on granulocytes, monocytes and macrophages but not on resting or stimulated lymphocytes.
• Synonyms: C5a-R, C5aR, C5AR1, Complement Component 5a Receptor 1
• Note: Clone 20/70 has been reported to block the binding of the C5a to murine CD88.