CD16 Mouse Monoclonal Antibody [Clone ID: G7]

CAT#: AM01051FC-N

CD16 mouse monoclonal antibody, clone G7, FITC

Specifications

Product Data

• Clone Name: G7
• Applications: FC
• Recommended Dilution: Flow cytometry: use 10 µl of neat antibody to label 10e6 cells in 100 µl.
• Reactivities: Porcine
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Porcine peripheral blood leucocytes.
• Specificity: This antibody recognises the porcine homologue of human CD16 (Fc gamma RIII alpha). CD16 is a low affinity FC gamma receptor expressed by polymorphonuclear cells (PMN), peripheral blood lymphocytes (PBL) and monocytes in pigs. Clone G7 has been reported to block PBL mediated antibody-dependent cell cytotoxicity (ADCC) and partially inhibit PMN-mediated ADCC.
• Formulation: PBS with 0.09% sodium azide as preservative and 1% BSA as stabilizer.
  Label: FITC
  State: Liquid purified IgG
  Label: Fluorescein Isothiocyanate Isomer 1
• Concentration: lot specific
• Purification: Protein G chromatography
• Conjugation: FITC
• Storage: Store the antibody undiluted at 2-8°C for up to one month or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
  This product is photosensitive and should be protected from light.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID Q28942
• Background: CD16 (FcgammaRIII) serves as a low affinity IgG receptor. Human CD16 is expressed in two forms CD16a and CD16b. CD16a is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. CD16b is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
• Synonyms: FCGR3A, CD16A, FCG3, FCGR3, IGFR3, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2