Phosphotyrosine Mouse Monoclonal Antibody [Clone ID: PY20]

CAT#: AM00199HR-N

Phosphotyrosine mouse monoclonal antibody, clone PY20, HRP

Specifications

Product Data

• Clone Name: PY20
• Applications: ELISA
• Recommended Dilution: ELISA: 1/1000 - 1/4000.
• Reactivities: Broad
• Host: Mouse
• Isotype: IgG2b
• Clonality: Monoclonal
• Immunogen: U266 cell line.
• Specificity: This antibody recognises phosphotyrosine, enabling the detection, characterisation and isolation of proteins containing phosphorylated tyrosine residues. The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the Tcell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyse the transfer of ATP to a tyrosine residue on specific cell protein targets. The binding of PY20 to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine.
• Formulation: 50%PBS, pH7.4 and 50% Glycerol
  Label: HRP
  State: Liquid purified IgG
  Label: Horseradish Peroxidase
• Purification: Affinity chromatography
• Conjugation: HRP
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.