malE Mouse Monoclonal Antibody [Clone ID: 6E4]

CAT#: AM00088PU-N

malE mouse monoclonal antibody, clone 6E4, Purified

Product Images

Antibody sensitivity: MBP was separated by SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with MBP antibody (6E4) Cat.-No AM00088PU-N (0.5 g/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: 100ng MBP Lane 2: 50ng Lan

Specifications

Product Data

• Clone Name: 6E4
• Applications: ELISA, WB
• Recommended Dilution: Western blot: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  ELISA: 0.05 µg/ml.
• Reactivities: Escherichia coli
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified MBP (Maltose Binding Protein)
• Specificity: This antibody can be used for the detection of MBP fusion proteins.
• Formulation: 1ml 2 x PBS containing 0.09% Sodium Azide, PEG and Sucrose
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 1 ml H₂O (15 min, RT)
• Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid Nitrogen) at -80°C.
  Avoid repeated freezing and thawing.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 2-8°C up to 3 months.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 43 kDa
• Database Link:
  UniProt ID P0AEX9
• Background: Maltose Binding Protein (MBP), the E. coli malE gene product, is widely used as fusion partner for the expression of recombinant proteins.
• Synonyms: MMBP, Maltodextrin-binding protein, malE-Tag