ERK2 (MAPK1) (C-term) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 6G11]

CAT#: AM00085PU-N

ERK2 (MAPK1) (C-term) (incl. pos. control) mouse monoclonal antibody, clone 6G11, Purified

Product Images

Detection of endogenous MAPK2: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab MAPK2-6G11 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: A431 Lane 2: A549 Lane 3: SKOV3 Lane 4: OVCAR5 Lane 5: HaCaT lane 6: PC3 Lane 7: HeLa Lane 8: HepG2

Specifications

Product Data

• Clone Name: 6G11
• Applications: ELISA, IP, WB
• Recommended Dilution: ELISA: Use at 0.05 µg/ml.
  Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer AS00002BU-N or AS00002BU-L.
  Immunoprecipitation: 1-10 µg/ml per 10⁶ pervanadate-treated A431 cells.
  Included Positive Control: Cell lysate from untreated HepG2 cells (See Protocols for more details).
• Reactivities: Canine, Human, Mouse, Rat
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide conjugated to KLH
  Epitope: C-terminus
• Specificity: This antibody specifically recognizes the C-terminus of MAP kinase 2 (ERK2).
  It does not crossreact with MAP kinase 1 (ERK1).
• Formulation: 1ml 2 x PBS containing PEG and Sucrose
  State: Purified
  State: Lyophilized purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Reconstitution Method: Restore with 1.0 ml H₂O (15 min, RT).
• Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for one month or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C to -80°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 42 kDa
• Gene Name: mitogen-activated protein kinase 1
• Database Link:
  Entrez Gene 5594 Human
  UniProt ID P28482
• Background: Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.
• Synonyms: Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2, MAPK2, MAPK1
• Note: Protocol: Positive Control Provided: Cell lysate from Untreated HepG2
  
  Description: Cell lysate from untreated HepG2 cells, hepatocellular carcinoma (human)
  
  Format: Lyophilized cell lysate from serum starved HepG2 cells.
  
  Reconstitution: Restore by addition of 200 µl H₂0. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Storage: Aliquote and store frozen.
  Avoid repeated freeze/thaw cycles.
  
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80.000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
  Note: The lyophilized cell lysates contain SDS and are not recommended for applications with native proteins such as immunoprecipitation.