EGFR (Extracell. Dom.) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 20E12]

CAT#: AM00046PU-N

EGFR (Extracell. Dom.) (incl. pos. control) mouse monoclonal antibody, clone 20E12, Purified

Product Images

Antibody sensitivity Whole cell lysates of A431cells containing defined cell numbers per lane were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab EGFR-20E12 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: 160.000 cells, lane 2: 80.000 cells lane 3: 40.000 cells, lane 4: 20.000 cells, lane 5: 10.000 cells, lane 6: 5.000 cells, lane 7: 2.500 cells, lane 8: 1.000 cells

Specifications

Product Data

• Clone Name: 20E12
• Applications: WB
• Recommended Dilution: Western blot (0.5 µg/ml for HRPO/ECL detection; recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer).
• Reactivities: Human
• Host: Mouse
• Isotype: IgG
• Clonality: Monoclonal
• Immunogen: Peptide conjugated to hemocyanin, extracellular domain
• Specificity: This antibody specifically recognizes the extracellular domain of EGF receptor.
• Formulation: PBS, 0.09 % Na-azide, PEG and Sucrose
  State: Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Subsequent ultrafiltration and size exclusion chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilised product upon arrival at -20 °C.
  Following reconstitution aliquot and store at 2 - 8 °C for up to three months or freeze in liquid nitrogen at -80 °C for longer. Avoid repeated freezing and thawing.
  Should this product contain a precipitate, we recommend centrifugation before use.
• Stability: Shelf life: One year from despatch.
• Gene Name: epidermal growth factor receptor
• Database Link:
  Entrez Gene 1956 Human
  UniProt ID P00533
• Background: EGF Receptor (EGFR) and erbB2, erbB3, and ErbB4 are members of subclass I of receptor tyrosine kinases.
  EGFR/erbB receptors are activated upon binding of EGF and EGF-related growth factors such as TGF alpha, beta-cellulin, Hb-EGF, HRG, or NRG. Binding of these ligands leads to receptor homo- and heterodimerization followed by autophosphorylation and activation of downstream signal transduction pathways (MAPK, PI3K/PKB, and STAT). In addition, EGFR becomes fully activated after phosphorylation of Y845 by src family kinases.
  Phosphorylation of Y1045 leads to association with cbl and subsequent receptor degradation. Phosphorylation of S1047 by CamKinase II leads to attenuation of kinase activity; phosphorylation of T654 (by PKC) and T669 (by MAPK, p38) interferes with receptor endocytosis/recycling.
• Synonyms: Epidermal growth factor receptor, EGF Receptor, erbB-1, c-ErbB-1
• Note: Mol. weight: 180 kDA.
  Positive control included: Cell lysate from untreated A431 cells.