N Cadherin (CDH2) (incl. pos. control) Mouse Monoclonal Antibody [Clone ID: 7E9]

CAT#: AM00013PU-N

N Cadherin (CDH2) (incl. pos. control) mouse monoclonal antibody, clone 7E9, Purified

Product Images

Detection of endogenous N-Cadherin: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to a PVDF membrane. The immunoblot was probed with mab NCAD-7E9 (0.5 ug/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Lane 1: HeLa Lane 2: HepG2 Lane 3: HEK293 Lane 4: SH-SY5Y Lane 5: MDCK Lane 6: PC12 Lane 7: CMT 93 Lane 8: Neuro 2A Lane 9: 3T3

Specifications

Product Data

• Clone Name: 7E9
• Applications: WB
• Recommended Dilution: Immunoblotting: 0.5 µg/ml for HRPO/ECL detection.
  Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
  Included Positive Control: Cell lysate from untreated SH-SY5Y cells (See Ptotocols for more details).
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Peptide conjugated to hemocyanin.
  Epitope: Cytoplasmic domain
• Specificity: This antibody specifically recognizes N-Cadherin at 127 kDa.
• Formulation: 1 ml 2 x PBS / 0.09% Sodium Azide / PEG and Sucrose
  State: Purified
  State: Lyophilized purified IgG fraction.
• Reconstitution Method: Restore with 1 ml H2O (15 min, RT).
• Purification: Subsequent Ultrafiltration and Size Exclusion Chromatography.
• Conjugation: Unconjugated
• Storage: Store lyophilized (preferably in a desiccator) at -20°C and reconstituted (aliquote and freeze in liquid nitrogen) at -80°C.
  Thaw aliquots at 37°C. Thawed aliquots may be stored at 4°C up to 3 months.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: cadherin 2
• Database Link:
  Entrez Gene 1000 Human
  UniProt ID P19022
• Background: Cadherins are a family of transmembrane glycoproteins that play a key role in calcium-dependent cell-cell adhesion. Several members of the cadherin family have been identified so far, including E- (epithelial), P- (placental), N- (neuronal), and M- (muscle) cadherin.
• Synonyms: CDH2, CDHN, NCAD, Neural cadherin, CDw325, Cadherin N, N-Cadherin
• Note: Protocol: Positive Control: Cell lysate from untreated SH-SY5Y cells
  
  Format: Lyophilized cell lysate from serum starved SH-SY5Y cells.
  
  Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200 µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min.
  
  Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20.000 cells.
  Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins.
  Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended for applications with native proteins such as in immunoprecipitation.
  
  Storage: Aliquote reconstituted product and store frozen. Avoid repeated fereezing and thawing.