AJUBA (NM_032876) Human Untagged Clone

CAT#: SC108799

AJUBA (untagged)-Human jub, ajuba homolog (Xenopus laevis) (JUB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AJUBA
• Synonyms: JUB
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_032876 edited
  GAATTCGGCACGAGGGAGGGGGCGAGAGGCCCCAGGCCCGAGGGCATGGAGCGGTTAGGA
  GAGAAAGCCAGTCGCCTGCTGGAGAAGTTCGGCCGCAGAAAGGGTGAATCTAGCCGGTCT
  GGGTCTGACGGGACCCCCGGGCCGGGCAAGGGGCGCCTAAGTGGGTTGGGGGGACCTAGG
  AAGTCAGGGCCCCGAGGAGCTACTGGGGGACCTGGGGATGAGCCGTTGGAGCCGGCCCGG
  GAGCAAGGTTCCCTGGACGCTGAGCGAAATCAGCGCGGCTCCTTTGAGGCGCCGCGCTAC
  GAAGGCTCTTTTCCCGCGGGGCCGCCGCCCACCCGGGCCTTGCCTCTACCTCAGTCGTTG
  CCCCCCGATTTTCGGCTGGAGCCCACGGCCCCGGCCCTCAGCCCCCGCTCTAGCTTCGCC
  AGTAGCTCGGCCAGCGACGCGAGCAAGCCGTCCAGCCCCCGGGGCAGCCTGCTGCTGGAC
  GGGGCGGGGGCTGGCGGAGCTGGAGGTAGCCGGCCCTGCAGCAATCGCACCAGCGGCATC
  AGCATGGGCTACGACCAGCGCCACGGGAGCCCCTTGCCAGCGGGGCCGTGCCTGTTTGGC
  CCACCCCTGGCCGGAGCACCGGCAGGCTATTCTCCCGGAGGGGTCCCGTCCGCCTACCCG
  GAGCTCCACGCCGCCCTGGACCGATTGTACGCTCAGCGGCCCGCGGGGTTCGGCTGCCAG
  GAAAGCCGCCACTCGTATCCCCCGGCCCTGGGCAGCCCTGGAGCTCTAGCCGGGGCCGGA
  GTGGGAGCGGCGGGGCCCTTGGAGAGACGGGGGGCGCAACCCGGACGACACTCTGTGACC
  GGCTACGGGGACTGCGCCGTGGGCGCCCGGTACCAGGACGAGCTAACAGCTTTGCTTCGC
  CTGACGGTGGGCACCGGTGGGCGAGAAGCCGGAGCCCGCGGAGAACCCTCGGGGATTGAG
  CCGTCGGGTCTGGAGGAGCCACCAGGTCCTTTCGTTCCGGAGGCCGCCCGGGCCCGGATG
  CGGGAGCCAGAGGCCAGGGAGGACTACTTCGGCACCTGTATCAAGTGCAACAAAGGCATC
  TATGGGCAGAGCAATGCCTGCCAGGCCCTGGACAGCCTCTACCACACCCAGTGCTTTGTT
  TGCTGCTCTTGTGGGCGAACTTTGCGTTGCAAGGCTTTCTACAGTGTCAATGGCTCTGTG
  TACTGTGAGGAAGATTATCTGTTTTCAGGGTTTCAGGAGGCAGCTGAGAAATGCTGTGTC
  TGTGGTCACTTGATTTTGGAGAAGATCCTACAAGCAATGGGGAAGTCCTATCATCCAGGC
  TGTTTCCGATGCATTGTTTGCAACAAGTGCCTGGATGGCATCCCCTTCACAGTGGACTTC
  TCCAACCAAGTATACTGTGTCACCGACTACCACAAAAATTATGCTCCTAAGTGTGCAGCC
  TGTGGCCAACCCATCCTCCCCTCTGAGGGCTGTGAGGACATCGTGAGGGTGATATCCATG
  GACCGGGATTATCACTTTGAGTGCTACCACTGTGAGGACTGCCGGATGCAGCTGAGTGAT
  GAGGAAGGCTGCTGCTGTTTCCCTCTGGATGGGCACTTGCTCTGCCATGGTTGCCACATG
  CAGCGGCTCAATGCCCGACAACCCCCTGCCAACTATATCTGAGCTGCAATCACTGCTGCT
  GCTGTCACCCTGCAGACAAACTGCTGTGGCCAGTGGGCCCCTCTGAGGCCAGCCAAGGCA
  AAGAATGCACTCTGCAGGCCTGGCAGAAGAGTCCTCTGGGGAGGACCCCAAGGGCCGGAG
  ACCCAAAGATCATGATATTCCAAATGGATTGTGGAAGAGAAACCTTATATTTACCAGGGT
  GGGGGCGACTGGCCTTTTTCCCATGTGTGCAGTCTGAGCTTAGGCACACACAGGAGGGTT
  CCAGGACTTTCTGAACATCTGATTCTGTATCTTCAGTATATATATTTTGTTTGTTTTAGA
  GATGGGATCTCACCATGTTGCCCAGGCTAGTCTTGAACTCCTGGGCTCGAATGATCCTCC
  CACCTTGGCCTCCCAAAGTGCTGGGATTATAGGCGTAAGCCACTGTGTCTGGCCTAGTGT
  ATGATTATGCATGAGTCACGCAATGTTCTGGTCCTGGATTCCAGGAGTAGAGGACCTAGC
  TTTAGATCAATTAGTTTCAGCTAAACTGACTGGAACCAGGTCAAAGTGTAATTCTCCCTC
  CAGCTCCCCCAAACCCCAGAGTTTTGGGGTTGTGGTTGATGCAGTGTGGGATGTCCCTGA
  GAGGTAGCAAGTCTAGGGTGGTGAGTTCCTGCTAGGCAACCAAATTTAAGCTCCTCACTT
  TTTGTGACACATGGTGTCAGATATGGGGTCCCGCACCTATATCTGGATGAAGAGGTAGAA
  ACTCTGGACCTCATTAATGAGTTATTTCTTGGCCTTCTTCTAAGGACTAGGAGAGCTCCT
  TATCTGTCTGAGAATGGGGACCAGCTCTGAGTGGGGTTGCTGCCTGTATTCCCTGTTTCT
  CAGGAACTTACATGGGTCTGGGGAGGCTAGGTAGGTGATTGTACGTGGTTGCTCTTCTCC
  TTGGCTGGGGGAGGTAATGAGCAGATCTCTGTGGGTGTGGAGCTTGTTGGGGGGATGTCT
  AGGAAGCTTCAGCTTAGCCACATTCCCAAGTTTAGGTGCACTGAGCCATATAGCCCAGTG
  TATGCATGTGTGGGTGTGTTCATGCACACACACACTCTCTCTCTTGTCTCTCTGTCTCTC
  TCTCACTCTTACTTTCTTACTCTCTTCTCAGGTCACTTGTACACTTGGTTTCCTAGTAGA
  AGCTCACTTGCCACCTCTCAGAGGGGTCCCGGATTGCATCCATCACAATCCCAAAACTAG
  AGTTGGGGGGAACTGGAGGGAGCAAAACACTGATTTGATACTAGTCAGTTTGCTTGAAAC
  TAGTTCACCTAAAGCTAGATCTCTTAAAACCAATTTACTGAAAACTTGTTTGCTTAAAGT
  TAATGACTTAATGACTAATTTGCCAAAAGCTCAATTCCTATTTTGGTGTGTTTATATCCA
  TTTAGGTGTCCTATTCTTTTTTGTCATGCTTTGGATATTTCAAGGATTTATATCTATTCA
  TCCAAGAGTACTTCTGAGTTATTATCAGCAACATAAATTTATCAAATTTGCAGCACTTTG
  TAAATGATGAGATTGCTTCCTACCTTTATGGATGTCTTTTTCTATGTTATCTACCATTCA
  AAAACTTTTTTAAAAAGTTTAAAGTTTCTAGCAATAAATACAATTGGTACAGACAAAAAA
  AAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_032876 unedited
  TTTGTCATAACCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGGAGGTCTA
  TATAAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGC
  GGCCGCGAATTCGGCACGAGGGAGGGGGCGAGAGGCCCCAGGCCCGAGGGCATGGAGCGG
  TTAGGAGAGAAAGCCAGTCGCCTGCTGGAGAAGTTCGGCCGCAGAAAGGGTGAATCTAGC
  CGGTCTGGGTCTGACGGGACCCCCGGGCCGGGCAAGGGGCGCCTAAGTGGGTTGGGGGGA
  CCTAGGAAGTCAGGGCCCCGAGGAGCTACTGGGGGACCTGGGGATGAGCCGTTGGAGCCG
  GCCCGGGAGCAAGGTTCCCTGGACGCTGAGCGAAATCAGCGCGGCTCCTTTGAGGCGCCG
  CGCTACGAAGGCTCTTTTCCCGCGGGGCCGCCGCCCACCCGGGCCTTGCCTCTACCTCAG
  TCGTTGCCCCCCGATTTTCGGCTGGAGCCCACGGCCCCGGCCCTCAGCCCCCGCTCTAGC
  TTCGCCAGTAGCTCGGCCAGCGACGCGAGCAAGCCGTCCAGCCCCCGGGGCAGCCTGCTG
  CTGGACGGGGCGGGGGCTGGCGGAGCTGGAGGTAGCCGGCCCTGCAGCAATCGCACCAGC
  GGCATCAGCATGGGCTACGACCAGCGCCACGGGAGCCCCTTGCCAGCGGGGCCGTGCCTG
  TTTGGCCCACCCCTGGCCGGAGCACCGGCAGGCTATTCTCCCGGGAGGGGTCCCGTCCGC
  CTACCCGGAGCTCCACGCGCCCTGGACCGATTGTACGCTCAGCGCCCGCGGGGTTCGGCT
  GCCAGGAAAGCCGCACTCCTATCCCCGGCCCTGGGCACCCTGGAGCCTAACCGGGCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_032876 unedited
  NNNNNNCCGGTTCCACTATGNNACCGCGGCCGCATNCTAGNGATCGGTTTTTTTTTTTTT
  TTTTTGTCTGTACCAATTGTATTTATTGCTAGAAACTTTAAACTTTTTAAAAAAGTTTTT
  GAATGGTAGATAACATAGAAAAAGACATCCATAAAGGTAGGAAGCAATCTCATCATTTAC
  AAAGTGCTGCAAATTTGATAAATTTATGTTGCTGATAATAACTCAGAAGTACTCTTGGAT
  GAATAGATATAAATCCTTGAAATATCCAAAGCATGACAAAAAAGAATAGGACACCTAAAT
  GGATATAAACACACCAAAATAGGAATTGAGCTTTTGGCAAATTAGTCATTAAGTCATTAA
  CTTTAAGCAAACAAGTTTTCAGTAAATTGGTTTTAAGAGATCTAGCTTTAGGTGAACTAG
  TTTCAAGCAAACTGACTAGTATCAAATCAGTGTTTTGCTCCCTCCAGTTCCCCCCAACTC
  TAGTTTTGGGATTGTGATGGATGCAATCCGGGACCCCTCTGAGAGGTGGCAAGTGAGCTT
  CTACTAGGAAACCAAGTGTACAAGTGACCTGAGAAGAGAGTAAGAAAGTAAGAGTGAGAG
  AGAGACAGAGAGACCAGAGAGAGAGTGTGTGTGTGCATGAACACACCCCCACATGCATAC
  ACTGGGGCTTTATGGCTCAGGGCCCCTCAAACTTGGGAATGTGGCTAAGCTGAAAGCTTC
  TAGACATCCCCCCACAAACTCCACACCCACAGAGATCTTGTATTTACCTCCCCCAGCCCA
  GGGGAAAAGCAACCCCTTCAAAAGCACTTACTTGGCCCTCCCCAAACCCGGGGAATTTCT
  TGGAAAACAGGGAATCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_032876
• Insert Size: 3600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032876.4, NP_116265.1
• RefSeq Size: 4236 bp
• RefSeq ORF: 1617 bp
• Locus ID: 84962
• UniProt ID: Q96IF1
• Cytogenetics: 14q11.2
• Domains: LIM
• Gene Summary: Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration. Contributes to the linking and/or strengthening of epithelia cell-cell junctions in part by linking adhesive receptors to the actin cytoskeleton. May be involved in signal transduction from cell adhesion sites to the nucleus. Plays an important role in regulation of the kinase activity of AURKA for mitotic commitment. Also a component of the IL-1 signaling pathway modulating IL-1-induced NFKB1 activation by influencing the assembly and activity of the PRKCZ-SQSTM1-TRAF6 multiprotein signaling complex. Functions as an HDAC-dependent corepressor for a subset of GFI1 target genes. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).