AJUBA (NM_032876) Human Tagged ORF Clone

CAT#: RC205482

AJUBA (Myc-DDK-tagged)-Human jub, ajuba homolog (Xenopus laevis) (JUB), transcript variant 1

Product Images

Circular map for RC205482

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  Western blot validation of overexpression lysate (Cat# LY409908) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC205482 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified AJUBA protein (Cat# TP305482). The protein was produced from HEK293T cells transfected with AJUBA cDNA clone (Cat# RC205482) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AJUBA
• Synonyms: JUB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205482 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCGGTTAGGAGAGAAAGCCAGTCGCCTGCTGGAGAAGTTCGGCCGCAGAAAGGGTGAATCTAGCC
  GGTCTGGGTCTGACGGGACCCCCGGGCCGGGCAAGGGGCGCCTAAGTGGGTTGGGGGGACCTAGGAAGTC
  AGGGCCCCGAGGAGCTACTGGGGGACCTGGGGATGAGCCGTTGGAGCCGGCCCGGGAGCAAGGTTCCCTG
  GACGCTGAGCGAAATCAGCGCGGCTCCTTTGAGGCGCCGCGCTACGAAGGCTCTTTTCCCGCGGGGCCGC
  CGCCCACCCGGGCCTTGCCTCTACCTCAGTCGTTGCCCCCCGATTTTCGGCTGGAGCCCACGGCCCCGGC
  CCTCAGCCCCCGCTCTAGCTTCGCCAGTAGCTCGGCCAGCGACGCGAGCAAGCCGTCCAGCCCCCGGGGC
  AGCCTGCTGCTGGACGGGGCGGGGGCTGGCGGAGCTGGAGGTAGCCGGCCCTGCAGCAATCGCACCAGCG
  GCATCAGCATGGGCTACGACCAGCGCCACGGGAGCCCCTTGCCAGCGGGGCCGTGCCTGTTTGGCCCACC
  CCTGGCCGGAGCACCGGCAGGCTATTCTCCCGGAGGGGTCCCGTCCGCCTACCCGGAGCTCCACGCCGCC
  CTGGACCGATTGTACGCTCAGCGGCCCGCGGGGTTCGGCTGCCAGGAAAGCCGCCACTCGTATCCCCCGG
  CCCTGGGCAGCCCTGGAGCTCTAGCCGGGGCCGGAGTGGGAGCGGCGGGGCCCTTGGAGAGACGGGGGGC
  GCAACCCGGACGACACTCTGTGACCGGCTACGGGGACTGCGCCGTGGGCGCCCGGTACCAGGACGAGCTA
  ACAGCTTTGCTTCGCCTGACGGTGGGCACCGGTGGGCGAGAAGCCGGAGCCCGCGGAGAACCCTCGGGGA
  TTGAGCCGTCGGGTCTGGAGGAGCCACCAGGTCCTTTCGTTCCGGAGGCCGCCCGGGCCCGGATGCGGGA
  GCCAGAGGCCAGGGAGGACTACTTCGGCACCTGTATCAAGTGCAACAAAGGCATCTATGGGCAGAGCAAT
  GCCTGCCAGGCCCTGGACAGCCTCTACCACACCCAGTGCTTTGTTTGCTGCTCTTGTGGGCGAACTTTGC
  GTTGCAAGGCTTTCTACAGTGTCAATGGCTCTGTGTACTGTGAGGAAGATTATCTGTTTTCAGGGTTTCA
  GGAGGCAGCTGAGAAATGCTGTGTCTGTGGTCACTTGATTTTGGAGAAGATCCTACAAGCAATGGGGAAG
  TCCTATCATCCAGGCTGTTTCCGATGCATTGTTTGCAACAAGTGCCTGGATGGCATCCCCTTCACAGTGG
  ACTTCTCCAACCAAGTATACTGTGTCACCGACTACCACAAAAATTATGCTCCTAAGTGTGCAGCCTGTGG
  CCAACCCATCCTCCCCTCTGAGGGCTGTGAGGACATCGTGAGGGTGATATCCATGGACCGGGATTATCAC
  TTTGAGTGCTACCACTGTGAGGACTGCCGGATGCAGCTGAGTGATGAGGAAGGCTGCTGCTGTTTCCCTC
  TGGATGGGCACTTGCTCTGCCATGGTTGCCACATGCAGCGGCTCAATGCCCGACAACCCCCTGCCAACTA
  TATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205482 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MERLGEKASRLLEKFGRRKGESSRSGSDGTPGPGKGRLSGLGGPRKSGPRGATGGPGDEPLEPAREQGSL
  DAERNQRGSFEAPRYEGSFPAGPPPTRALPLPQSLPPDFRLEPTAPALSPRSSFASSSASDASKPSSPRG
  SLLLDGAGAGGAGGSRPCSNRTSGISMGYDQRHGSPLPAGPCLFGPPLAGAPAGYSPGGVPSAYPELHAA
  LDRLYAQRPAGFGCQESRHSYPPALGSPGALAGAGVGAAGPLERRGAQPGRHSVTGYGDCAVGARYQDEL
  TALLRLTVGTGGREAGARGEPSGIEPSGLEEPPGPFVPEAARARMREPEAREDYFGTCIKCNKGIYGQSN
  ACQALDSLYHTQCFVCCSCGRTLRCKAFYSVNGSVYCEEDYLFSGFQEAAEKCCVCGHLILEKILQAMGK
  SYHPGCFRCIVCNKCLDGIPFTVDFSNQVYCVTDYHKNYAPKCAACGQPILPSEGCEDIVRVISMDRDYH
  FECYHCEDCRMQLSDEEGCCCFPLDGHLLCHGCHMQRLNARQPPANYI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032876
• ORF Size: 1614 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032876.6
• RefSeq Size: 4262 bp
• RefSeq ORF: 1617 bp
• Locus ID: 84962
• UniProt ID: Q96IF1
• Cytogenetics: 14q11.2
• Domains: LIM
• MW: 56.9 kDa
• Gene Summary: Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, mitosis, cell-cell adhesion, cell differentiation, proliferation and migration. Contributes to the linking and/or strengthening of epithelia cell-cell junctions in part by linking adhesive receptors to the actin cytoskeleton. May be involved in signal transduction from cell adhesion sites to the nucleus. Plays an important role in regulation of the kinase activity of AURKA for mitotic commitment. Also a component of the IL-1 signaling pathway modulating IL-1-induced NFKB1 activation by influencing the assembly and activity of the PRKCZ-SQSTM1-TRAF6 multiprotein signaling complex. Functions as an HDAC-dependent corepressor for a subset of GFI1 target genes. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1.[UniProtKB/Swiss-Prot Function]