WDR23 (DCAF11) (NM_025230) Human Tagged ORF Clone

CAT#: RG209449

DCAF11 (tGFP-tagged) - Human DDB1 and CUL4 associated factor 11 (DCAF11), transcript variant 1

Product Images

Circular map for RG209449

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: WDR23
• Synonyms: GL014; PRO2389; WDR23
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG209449 representing NM_025230
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATCGCGGAACAGCAGCAGTGCAGGATCCGGGTCCGGAGACCCCTCCGAGGGCTTGCCCCGAAGAG
  GGGCTGGCCTGCGTCGGAGTGAGGAAGAGGAAGAAGAGGATGAAGATGTGGATCTGGCCCAGGTACTGGC
  CTATCTCCTCCGCAGAGGCCAAGTGAGGTTGGTGCAGGGAGGAGGTGCAGCAAATTTACAATTCATTCAG
  GCCCTCTTGGACTCAGAGGAAGAGAATGACAGAGCTTGGGATGGTCGTCTTGGGGATCGATACAACCCAC
  CTGTGGATGCTACCCCTGACACCCGGGAGCTGGAATTCAATGAGATCAAGACACAAGTGGAACTGGCCAC
  AGGGCAGCTGGGGCTTAGGCGGGCCGCCCAGAAGCACAGCTTTCCTCGAATGTTGCACCAGAGAGAACGG
  GGCCTCTGCCATCGGGGAAGCTTCTCCCTTGGAGAACAGTCTCGAGTGATATCTCACTTCTTGCCCAATG
  ATCTGGGCTTCACTGATAGCTACTCTCAGAAGGCTTTCTGTGGCATCTACAGCAAAGATGGTCAAATATT
  CATGTCTGCTTGCCAAGACCAGACAATCCGACTCTATGACTGCCGATATGGCCGTTTCCATAAATTCAAG
  AGCATCAAGGCCCGCGATGTAGGCTGGAGCGTCTTGGATGTGGCCTTCACCCCTGATGGGAACCACTTCC
  TCTACTCTAGCTGGTCTGATTACATTCATATCTGCAATATCTATGGTGAGGGAGATACACACACTGCCCT
  GGATCTCAGGCCAGATGAGCGTCGCTTTGCTGTCTTCTCCATTGCTGTCTCCTCAGATGGACGAGAAGTA
  CTAGGAGGGGCCAATGATGGCTGCCTGTATGTCTTTGACCGAGAACAGAACCGGCGCACCCTTCAGATTG
  AGTCCCATGAGGATGATGTGAATGCAGTGGCCTTTGCTGATATAAGCTCCCAAATCCTGTTCTCTGGGGG
  AGATGATGCCATCTGCAAAGTGTGGGATCGACGCACCATGCGGGAGGATGACCCCAAGCCTGTGGGTGCA
  CTGGCTGGACACCAGGATGGCATCACCTTCATTGACAGCAAGGGTGATGCCCGGTATCTGATCTCCAACT
  CTAAAGACCAGACCATCAAACTCTGGGATATCCGACGCTTTTCCAGCCGGGAAGGCATGGAAGCTTCACG
  CCAGGCTGCCACACAGCAAAACTGGGACTATCGGTGGCAGCAAGTGCCCAAAAAAGCCTGGCGGAAGCTG
  AAGCTCCCAGGGGACAGCTCCTTGATGACCTACCGGGGCCACGGAGTGCTGCACACCCTCATCCGCTGCC
  GGTTCTCCCCCATTCATAGCACTGGCCAGCAGTTCATCTACAGTGGCTGCTCCACTGGCAAAGTGGTTGT
  GTACGACCTTCTAAGTGGCCACATTGTGAAGAAGCTGACCAACCACAAGGCCTGTGTGCGTGACGTCAGT
  TGGCACCCCTTTGAAGAGAAGATTGTCAGCAGTTCGTGGGACGGGAACCTGCGTCTGTGGCAGTACCGCC
  AGGCTGAGTACTTCCAGGATGACATGCCAGAATCTGAGGAATGTGCCAGCGCCCCTGCCCCAGTGCCCCA
  ATCCTCTACACCCTTTTCCTCACCCCAG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG209449 representing NM_025230
  Red=Cloning site Green=Tags(s)
  
  MGSRNSSSAGSGSGDPSEGLPRRGAGLRRSEEEEEEDEDVDLAQVLAYLLRRGQVRLVQGGGAANLQFIQ
  ALLDSEEENDRAWDGRLGDRYNPPVDATPDTRELEFNEIKTQVELATGQLGLRRAAQKHSFPRMLHQRER
  GLCHRGSFSLGEQSRVISHFLPNDLGFTDSYSQKAFCGIYSKDGQIFMSACQDQTIRLYDCRYGRFHKFK
  SIKARDVGWSVLDVAFTPDGNHFLYSSWSDYIHICNIYGEGDTHTALDLRPDERRFAVFSIAVSSDGREV
  LGGANDGCLYVFDREQNRRTLQIESHEDDVNAVAFADISSQILFSGGDDAICKVWDRRTMREDDPKPVGA
  LAGHQDGITFIDSKGDARYLISNSKDQTIKLWDIRRFSSREGMEASRQAATQQNWDYRWQQVPKKAWRKL
  KLPGDSSLMTYRGHGVLHTLIRCRFSPIHSTGQQFIYSGCSTGKVVVYDLLSGHIVKKLTNHKACVRDVS
  WHPFEEKIVSSSWDGNLRLWQYRQAEYFQDDMPESEECASAPAPVPQSSTPFSSPQ
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_025230
• ORF Size: 1638 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025230.3, NP_079506.3
• RefSeq Size: 4286 bp
• RefSeq ORF: 1641 bp
• Locus ID: 80344
• UniProt ID: Q8TEB1
• Cytogenetics: 14q12
• Domains: WD40
• Gene Summary: This gene encodes a WD repeat-containing protein that interacts with the COP9 signalosome, a macromolecular complex that interacts with cullin-RING E3 ligases and regulates their activity by hydrolyzing cullin-Nedd8 conjugates. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2009]