NOLC1 (NM_004741) Human Tagged ORF Clone

CAT#: RC200356

NOLC1 (Myc-DDK-tagged)-Human nucleolar and coiled-body phosphoprotein 1 (NOLC1)

Product Images

Circular map for RC200356

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  Western blot validation of overexpression lysate (Cat# LY401495) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200356 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NOLC1 protein (Cat# TP300356). The protein was produced from HEK293T cells transfected with NOLC1 cDNA clone (Cat# RC200356) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NOLC1
• Synonyms: NOPP130; NOPP140; NS5ATP13; P130; Srp40
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200356 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGACGCCGGCATTCGCCGCGTGGTTCCCAGCGACCTGTATCCCCTCGTGCTCGGCTTCCTGCGCG
  ATAACCAACTCTCAGAGGTGGCCAATAAGTTCGCCAAAGCGACAGGAGCTACACAGCAGGATGCCAATGC
  CTCTTCCCTCTTAGACATCTATAGCTTCTGGCTCAACAGGTCTGCCAAGGTCCCAGAGCGAAAGTTACAG
  GCAAATGGACCAGTGGCTAAGAAAGCTAAGAAGAAGGCCTCATCCAGTGACAGTGAGGACAGCAGCGAGG
  AGGAGGAGGAAGTTCAAGGGCCTCCAGCAAAGAAGGCTGCTGTACCTGCCAAGCGAGTCGGTCTGCCTCC
  TGGGAAGGCTGCAGCCAAAGCATCAGAGAGTAGCAGCAGTGAAGAGTCCAGTGATGATGATGATGAGGAG
  GACCAAAAGAAACAGCCTGTCCAGAAGGGAGTTAAGCCCCAAGCCAAGGCAGCCAAAGCTCCTCCTAAGA
  AGGCCAAGAGCTCTGATTCTGATTCTGACTCAAGCTCCGAGGATGAGCCACCAAAGAACCAGAAGCCAAA
  GATAACACCTGTGACAGTTAAAGCTCAGACTAAAGCCCCTCCCAAACCAGCTCGAGCAGCACCTAAAATA
  GCCAATGGTAAAGCAGCCAGTAGCAGCAGTAGCAGCAGCAGCAGCAGTAGCAGTGATGACTCAGAGGAGG
  AGAAGGCAGCAGCCACCCCCAAGAAGACTGTACCTAAAAAGCAAGTTGTGGCCAAGGCCCCAGTGAAAGC
  AGCTACCACCCCTACCCGGAAGAGTTCTAGCAGTGAGGATTCCTCCAGTGACGAGGAAGAGGAGCAAAAA
  AAACCCATGAAAAATAAACCAGGTCCCTACAGTTCAGTCCCCCCGCCTTCTGCTCCCCCACCAAAGAAGT
  CTCTGGGAACCCAGCCTCCCAAGAAGGCTGTGGAGAAGCAGCAGCCTGTGGAAAGCAGTGAAGACAGCAG
  TGATGAGTCTGATTCAAGTTCTGAAGAAGAGAAGAAACCCCCAACTAAGGCAGTAGTCTCTAAAGCAACC
  ACTAAACCACCTCCAGCAAAGAAAGCAGCAGAGAGCTCTTCAGACAGCTCAGACTCTGACAGCTCTGAGG
  ATGATGAAGCTCCTTCTAAGCCAGCTGGTACCACCAAGAATTCTTCAAATAAGCCAGCTGTCACCACCAA
  GTCACCTGCAGTGAAGCCAGCTGCAGCCCCCAAGCAACCTGTGGGCGGTGGCCAGAAGCTTCTGACGAGA
  AAGGCTGACAGCAGCTCCAGTGAGGAAGAGAGCAGCTCCAGTGAGGAGGAGAAGACAAAGAAGATGGTGG
  CCACCACTAAGCCCAAGGCGACTGCCAAAGCAGCTCTATCTCTGCCTGCCAAGCAGGCTCCTCAGGGTAG
  TAGGGACAGCAGCTCTGATTCAGACAGCTCCAGCAGTGAGGAGGAGGAAGAGAAGACATCTAAGTCTGCA
  GTTAAGAAGAAGCCACAGAAGGTAGCAGGAGGTGCAGCCCCTTCCAAGCCAGCCTCTGCAAAGAAAGGAA
  AGGCTGAGAGCAGCAACAGTTCTTCTTCTGATGACTCCAGTGAGGAAGAGGAAGAGAAGCTCAAGGGCAA
  GGGCTCTCCAAGACCACAAGCCCCCAAGGCCAATGGCACCTCTGCACTGACTGCCCAGAATGGAAAAGCA
  GCTAAGAACAGTGAGGAGGAGGAAGAAGAAAAGAAAAAGGCGGCAGTGGTAGTTTCCAAATCAGGTTCAT
  TAAAGAAGCGGAAGCAGAATGAGGCTGCCAAGGAGGCAGAGACTCCTCAGGCCAAGAAGATAAAGCTTCA
  GACCCCTAACACATTTCCAAAAAGGAAGAAAGGAGAAAAAAGGGCATCATCCCCATTCCGAAGGGTCAGG
  GAGGAGGAAATTGAGGTGGATTCACGAGTTGCGGACAACTCCTTTGATGCCAAGCGAGGTGCAGCCGGAG
  ACTGGGGAGAGCGAGCCAATCAGGTTTTGAAGTTCACCAAAGGCAAGTCCTTTCGGCATGAGAAAACCAA
  GAAGAAGCGGGGCAGCTACCGGGGAGGCTCAATCTCTGTCCAGGTCAATTCTATTAAGTTTGACAGCGAG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200356 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MADAGIRRVVPSDLYPLVLGFLRDNQLSEVANKFAKATGATQQDANASSLLDIYSFWLNRSAKVPERKLQ
  ANGPVAKKAKKKASSSDSEDSSEEEEEVQGPPAKKAAVPAKRVGLPPGKAAAKASESSSSEESSDDDDEE
  DQKKQPVQKGVKPQAKAAKAPPKKAKSSDSDSDSSSEDEPPKNQKPKITPVTVKAQTKAPPKPARAAPKI
  ANGKAASSSSSSSSSSSSDDSEEEKAAATPKKTVPKKQVVAKAPVKAATTPTRKSSSSEDSSSDEEEEQK
  KPMKNKPGPYSSVPPPSAPPPKKSLGTQPPKKAVEKQQPVESSEDSSDESDSSSEEEKKPPTKAVVSKAT
  TKPPPAKKAAESSSDSSDSDSSEDDEAPSKPAGTTKNSSNKPAVTTKSPAVKPAAAPKQPVGGGQKLLTR
  KADSSSSEEESSSSEEEKTKKMVATTKPKATAKAALSLPAKQAPQGSRDSSSDSDSSSSEEEEEKTSKSA
  VKKKPQKVAGGAAPSKPASAKKGKAESSNSSSSDDSSEEEEEKLKGKGSPRPQAPKANGTSALTAQNGKA
  AKNSEEEEEEKKKAAVVVSKSGSLKKRKQNEAAKEAETPQAKKIKLQTPNTFPKRKKGEKRASSPFRRVR
  EEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFDSE
  
  SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004741
• ORF Size: 2100 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004741.5
• RefSeq Size: 3947 bp
• RefSeq ORF: 2100 bp
• Locus ID: 9221
• UniProt ID: Q14978
• Cytogenetics: 10q24.32
• Domains: SRP40_C
• Protein Families: Stem cell - Pluripotency
• MW: 73.7 kDa
• Gene Summary: Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:10567578, PubMed:26399832). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with TCOF1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832). Involved in nucleologenesis, possibly by playing a role in the maintenance of the fundamental structure of the fibrillar center and dense fibrillar component in the nucleolus (PubMed:9016786). It has intrinsic GTPase and ATPase activities (PubMed:9016786).[UniProtKB/Swiss-Prot Function]