Orexin Receptor 1 (HCRTR1) (NM_001525) Human Untagged Clone

CAT#: SC315431

HCRTR1 (untagged)-Human hypocretin (orexin) receptor 1 (HCRTR1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Orexin Receptor 1
• Synonyms: ORXR1; OX1R; OXR1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001525 edited
  GGGGAGGGGCGGGGAGCTGGGCTGGCTGGATTTATGAATGGAGAGCGACCCGCGCGCCGG
  AACAGCGGCTCCTGGCGGCCGTCGGGGAGCGTCGCGGCCCTGGGGACCCAAAGGGGCCTC
  TTAGGGGGCCTGGATGCTCCCCTTGCTCGCAAGGGGTCGTCAGTCCCTCCGCGCACCACC
  CCCACTGTGTGTGTGTTGTATGTGTGCGTGTCCGCGAGTGCCCACCGGAGGGTCTGGCAG
  GTATGGCGGGTGGGGCTTGGGTCTCTAACACCTCCCTTGGCCCGTTTTCCCAACCCAAAG
  TTAAAGCCTCTGAACTGGCTCAAGAAATATTTGCAATCGGGATGGCTTCTCCTCCCAAAT
  CTACGGTGTTTGGTGGGTTCGAACAGACCTGGCTTAAGAGCTTTGTGACCTTGAGCAAGA
  GACTCAATCTCTCTGAGCTTCGGTCTCATCTGCAAAGCAGGGTACCCTAATAATGGTAAC
  CGGAAACGTCCCCGAAACTACCTTCTCGTACCAGGTTCTTGGTGAAGCACTTGGCACGCA
  TCGGAGCTCATTACTCCTCATCGTGGTCCTGTAAGGTATGTAGGGCTGTCACCCCATTAG
  ACAGATGGGGAAACCAAGGCTGAAAGAGGCCAGGTAAGCTACCCAAGGCAACTGGTGTGG
  AATTGGGATGCAACCCAGGTCTGTCTTCCTCCACCAATTTCATGACTGTGAGAATTAAGA
  GGGAACTTATACGCAAAGCGCCTGGCACAATCCCTAATGTTTCCTTCCTTCTCTCTTTTC
  CCACTCCTCCTTTCCTTCCTCCCTTCAGGAAGTTTGAGGCTGAGACCCGAAAAGACCTGG
  GTGCAAGCCTCCAGGCACCCTGAAGGGAGTGGGCTGAGGGCTGGCCCAAGCTCCCTCCTC
  TCCCTCTGTAGAGCCTAGGATGCCCCTCTGCTGCAGCGGCTCCTGAGCTCATGGAGCCCT
  CAGCCACCCCAGGGGCCCAGATGGGGGTCCCCCCTGGCAGCAGAGAGCCGTCCCCTGTGC
  CTCCAGACTATGAAGATGAGTTTCTCCGCTATCTGTGGCGTGATTATCTGTACCCAAAAC
  AGTATGAGTGGGTCCTCATCGCAGCCTATGTGGCTGTGTTCGTCGTGGCCCTGGTGGGCA
  ACACGCTGGTCTGCCTGGCCGTGTGGCGGAACCACCACATGAGGACAGTCACCAACTACT
  TCATTGTCAACCTGTCCCTGGCTGACGTTCTGGTGACTGCTATCTGCCTGCCGGCCAGCC
  TGCTGGTGGACATCACTGAGTCCTGGCTGTTCGGCCATGCCCTCTGCAAGGTCATCCCCT
  ATCTACAGGCTGTGTCCGTGTCAGTGGCAGTGCTAACTCTCAGCTTCATCGCCCTGGACC
  GCTGGTATGCCATCTGCCACCCACTATTGTTCAAGAGCACAGCCCGGCGGGCCCGTGGCT
  CCATCCTGGGCATCTGGGCTGTGTCGCTGGCCATCATGGTGCCCCAGGCTGCAGTCATGG
  AATGCAGCAGTGTGCTGCCTGAGCTAGCCAACCGCACACGGCTCTTCTCAGTCTGTGATG
  AACGCTGGGCAGATGACCTCTATCCCAAGATCTACCACAGTTGCTTCTTTATTGTCACCT
  ACCTGGCCCCACTGGGCCTCATGGCCATGGCCTATTTCCAGATATTCCGCAAGCTCTGGG
  GCCGCCAGATCCCCGGCACCACCTCAGCACTGGTGCGGAACTGGAAGCGCCCCTCAGACC
  AGCTGGGGGACCTGGAGCAGGGCCTGAGTGGAGAGCCCCAGCCCCGGGCCCGCGCCTTCC
  TGGCTGAAGTGAAGCAGATGCGTGCACGGAGGAAGACAGCCAAGATGCTGATGGTGGTGC
  TGCTGGTCTTCGCCCTCTGCTACCTGCCCATCAGCGTCCTCAATGTCCTTAAGAGGGTGT
  TCGGGATGTTCCGCCAAGCCAGTGACCGCGAAGCTGTCTACGCCTGCTTCACCTTCTCCC
  ACTGGCTGGTGTACGCCAACAGCGCTGCCAACCCCATCATCTACAACTTCCTCAGTGGCA
  AATTCCGGGAGCAGTTTAAGGCTGCCTTCTCCTGCTGCCTGCCTGGCCTGGGTCCCTGCG
  GCTCTCTGAAGGCCCCTAGTCCCCGCTCCTCTGCCAGCCACAAGTCCTTGTCCTTGCAGA
  GCCGATGCTCCGTCTCCAAAATCTCTGAGCATGTGGTGCTCACCAGCGTCACCACAGTGC
  TGCCCTGAGCGAGGGCTGCCCTGGAGGCTCCGGCTCGGGGGATCTGCCCCTACCCCTCAT
  GGAAAGACAGCTGGATGTGGTGAAAGGCTGTGGCTTCAGTCCTGGGTTTCTGCCTGTGTG
  ACTCTGGATAAGTCACTTCCTCTGTCTGAGCTTGTGTCCCCGAAGCAGGGTTGATGTGAG
  GATTAAGCATGCTGAAGCAAGTGGAAAGCTCCTTGTAAACTGTGAAGTGTTGTGGACATG
  AAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001525
• Insert Size: 2500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone is found to be a good match to NM_001525.1 except for 2 SNPs.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001525.1, NP_001516.1
• RefSeq Size: 1564 bp
• RefSeq ORF: 1278 bp
• Locus ID: 3061
• UniProt ID: O43613
• Cytogenetics: 1p35.2
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B. [provided by RefSeq, Jan 2009]