Orexin Receptor 1 (HCRTR1) Goat Polyclonal Antibody

CAT#: TA303362

Goat Polyclonal Antibody against HCRTR1

Product Images

TA303362 (0.5ug/ml) staining of Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• 

  Figure from citation: Western Blot of HCRTR1 protein level by using anti-HCRTR1 antibody in mouse membrane fractions. Dilution: 1:500 View Citation

• 

  ICC analysis of HCRTR-1 expression by using anti-HCRTR1 antibody. In layer V of SS (C), the HCRTR-1 granular immunoreactivity was detected at the level of neuronal cell bodies based on morphology (arrows). (C') gives a higher magnification detail of (C). In layer V of Fr2 (D), the HCRTR-1 positive (+) signal was more intense than in SS (arrows and inset in D'), but also in neuropilar processes of different caliber (arrowheads). (D') gives a higher magnification detail of (C). Dilution: 1:150

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ELISA: 1:32,000. WB: 0.3-1µg/ml.
• Reactivities: Human, Mouse, Rat (Expected from sequence similarity: Dog, Pig, Cow)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-YNFLSGKFREQFK, from the internal region of the protein sequence according to NP_001516.1; NP_001517.1.
• Formulation: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: hypocretin receptor 1
• Database Link:
  NP_001516
  Entrez Gene 25593 RatEntrez Gene 230777 MouseEntrez Gene 100855896 DogEntrez Gene 3061 Human
  UniProt ID O43613
• Background: The protein encoded by this gene is a G-protein coupled receptor involved in the regulation of feeding behavior. The encoded protein selectively binds the hypothalamic neuropeptide orexin A. A related gene (HCRTR2) encodes a G-protein coupled receptor that binds orexin A and orexin B. [provided by RefSeq]
• Synonyms: OX1R

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction