NF-kB p65 (RELA) Human Gene Knockout Kit (CRISPR)

CAT#: KN220780RB

RELA - human gene knockout kit via CRISPR, HDR mediated

Product Images

Specifications

Product Data

• Format: 2 gRNA vectors, 1 RFP-BSD donor, 1 scramble control
• Donor DNA: RFP-BSD
• Symbol: NF-kB p65
• Locus ID: 5970
• Components:

  KN220780G1, NF-kB p65 gRNA vector 1 in pCas-Guide CRISPR vector, Target Sequence: CCCGCGGGGTGGCATCGCCG

  KN220780G2, NF-kB p65 gRNA vector 2 in pCas-Guide CRISPR vector, Target Sequence: GGGGCCGCCGCGGCTGTGGC

  KN220780RBD, donor DNA containing left and right homologous arms and RFP-BSD functional cassette.

  Homologous arm and RFP-BSD sequences:

  pUC vector backbone in gray; Left arm sequence in blue; RFP-BSD in green; Right arm in violet

  CCGCATCTAG ATTGGGGTGG GTAGGACATT TTAACGTGCT TCTGAGACAG CCACCACCGA AAGGCACCTT TAGCGGTTAG GAAACCCGCG CGGAGTTCAA CCACCCGGCC TCTGCGGAGC AGAAGGCCCG GTCGGGCTTT TCCCTGCGCG GGGCGGGCGG CCGCAGCCCA GTAGCCCTGG CTTTGCTCCA GACCCGGCCC AGAGTGACAT CACCAAACTC CGCCGATCGG CGGGAGGGGG CCCTGAAATC CCCTAAAAAC AAAGTGAGTA ATCGGCGGAC CCACCCTCCA GGCGGGGCCG GGACCCGGGA GCTAGCCGAG CCACCGGGCA CGCCAGCCTC TTCGTCCTCG GCGAGGCGCG CACTTGGCCC CGACCCCCGG CAGCGGCTGT GCGTGCAGCC TCTTCGTCCT CCGCGCGGCG TGCACTTGCT CCCGGCCCCT GCGCCGGGCG GCGGCGGGGC AGCGCGCAGG CGCGGCCGGA TTCCGGGCAG TGACGCGACG GCGGGCCGCG CGGCGCATTT CCGCCTCTGG CGAATGGCTC GTCTGTAGTG CACGCCGCGG GCCCAGCTGC GACCCCGGCC CCGCCCCCGG GACCCCGGCC GCTTTCCGCC CCTTCCTGCG CCGACGGGCG CTGCGCAGGG AGCGGTCGGC CTGCCGGATG GTGGCGCGGG GCGGGGCAGG CGGGGAGGGG TCTGACTCAG TTTCCCCTCT GGGTGGAGGG GGTGGCCCTT GACTCAGCAT CCTCCCTGGG TGGGGGAGCA GAGGGAACCT TGACTCAGTT TCCCTCCACA CCGTCCATGG GGGCAGCTCT GGCTTCGTTT CCCTATTAGG GGAGAACGCA TCTGATTCAG TTTCCTCTCT GGAGGGAGGT GGGGCGTGCC CTCCCTCAAT TTTCCTTAAG AAAGTACAAC AGGCCCTGAT TCAGCTTCCC TCTGGAGAAG AAGGATTCCT TACCCCGTTT TCCCTCAAGA AGCAGGTGTT TCTGGTTCCT TTCCTCCACC CCTTAGTTTC ACCGCAGGTT CTAGGGAGCA GGTCCTGACT CAGTTCCCCT GGGAGGGAGC CTGGCTTCTC CTCTCCGTGG AGAGCAGATT CCTCCTTGTG AGAATGGGCA TTGGGTGTGT GCTGACCCTG ATCTCCCTCC CCTTTCAGAA CTGTTCCCCC TCATCTTCCC GGCAGGTAAG TGGCCCCCGC GGCGGTCGGG

  GE100003, scramble sequence in pCas-Guide vector

• Disclaimer: These products are manufactured and supplied by OriGene under license from ERS. The kit is designed based on the best knowledge of CRISPR technology. The system has been functionally validated for knocking-in the cassette downstream the native promoter. The efficiency of the knock-out varies due to the nature of the biology and the complexity of the experimental process.

Reference Data

• RefSeq: NM_001145138, NM_001243984, NM_001243985, NM_021975
• UniProt ID: Q04206
• Synonyms: NFKB3; p65
• Summary: NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]