KLF4 (NM_004235) Human Untagged Clone

CAT#: SC327870

KLF4 (untagged)-Human Kruppel-like factor 4 (gut) (KLF4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KLF4
• Synonyms: EZF; GKLF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004235 edited
  ACCAGAGAGAACGAACGTGTCTGCGGGCGCGCGGGGAGCAGAGGCGGTGGCGGGCGGCGG
  CGGCACCGGGAGCCGCCGAGTGACCCTCCCCGCCCCTCTGGCCCCCCACCCTCCCACCCG
  CCCGTGGCCCGCGCCCATGGCCGCGCGCGCTCCACACAACTCACCGGAGTCCGCGCCYTG
  CGCCGCCGACCAGTTCGCAGCTCCGCGCCACGGCAGCCAGTCTCACCTGGCGGCACCGCC
  CGCCCACCGCCCCGGCCACAGCCCCTGCGCCCACGGCAGCACTCGAGGCGACCGCGACAG
  TGGTGGGGGACGCTGCTGAGTGGAAGAGAGCGCAGCCCGGCCACCGGACCTACTTACTCG
  CCTTGCTGATTGTCTATTTTTGCGTTTACAACTTTTCTAAGAACTTTTGTATACAAAGGA
  ACTTTTTAAAAAAGACGCTTCCAAGTTATATTTAATCCAAAGAAGAAGGATCTCGGCCAA
  TTTGGGGTTTTGGGTTTTGGCTTCGTTTCTTCTCTTCGTTGACTTTGGGGTTCAGGTGCC
  CCAGCTGCTTCGGGCTGCCGAGGACCTTCTGGGCCCCCACATTAATGAGGCAGCCACCTG
  GCGAGTCTGACATGGCTGTCAGCGACGCGCTGCTCCCATCTTTCTCCACGTTCGCGTCTG
  GCCCGGCGGGAAGGGAGAAGACACTGCGTCAAGCAGGTGCCCCGAATAACCGCTGGCGGG
  AGGAGCTCTCCCACATGAAGCGACTTCCCCCAGTGCTTCCCGGCCGCCCCTATGACCTGG
  CGGCGGCGACCGTGGCCACAGACCTGGAGAGCGGCGGAGCCGGTGCGGCTTGCGGCGGTA
  GCAACCTGGCGCCCCTACCTCGGAGAGAGACCGAGGAGTTCAACGATCTCCTGGACCTGG
  ACTTTATTCTCTCCAATTCGCTGACCCATCCTCCGGAGTCAGTGGCCGCCACCGTGTCCT
  CGTCAGCGTCAGCCTCCTCTTCGTCGTCGCCGTCGAGCAGCGGCCCTGCCAGCGCGCCCT
  CCACCTGCAGCTTCACCTATCCGATCCGGGCCGGGAACGACCCGGGCGTGGCGCCGGGCG
  GCACGGGCGGAGGCCTCCTCTATGGCAGGGAGTCCGCTCCCCCTCCGACGGCTCCCTTCA
  ACCTGGCGGACATCAACGACGTGAGCCCCTCGGGCGGCTTCGTGGCCGAGCTCCTGCGGC
  CAGAATTGGACCCGGTGTACATTCCGCCGCAGCAGCCGCAGCCGCCAGGTGGCGGGCTGA
  TGGGCAAGTTCGTGCTGAAGGCGTCGCTGAGCGCCCCTGGCAGCGAGTACGGCAGCCCGT
  CGGTCATCAGCGTCAGCAAAGGCAGCCCTGACGGCAGCCACCCGGTGGTGGTGGCGCCCT
  ACAACGGCGGGCCGCCGCGCACGTGCCCCAAGATCAAGCAGGAGGCGGTCTCTTCGTGCA
  CCCACTTGGGCGCTGGACCCCCTCTCAGCAATGGCCACCGGCCGGCTGCACACGACTTCC
  CCCTGGGGCGGCAGCTCCCCAGCAGGACTACCCCGACCCTGGGTCTTGAGGAAGTGCTGA
  GCAGCAGGGACTGTCACCCTGCCCTGCCGCTTCCTCCCGGCTTCCATCCCCACCCGGGGC
  CCAATTACCCATCCTTCCTGCCCGATCAGATGCAGCCGCAAGTCCCGCCGCTCCATTACC
  AAGAGCTCATGCCACCCGGTTCCTGCATGCCAGAGGAGCCCAAGCCAAAGAGGGGAAGAC
  GATCGTGGCCCCGGAAAAGGACCGCCACCCACACTTGTGATTACGCGGGCTGCGGCAAAA
  CCTACACAAAGAGTTCCCATCTCAAGGCACACCTGCGAACCCACACAGGTGAGAAACCTT
  ACCACTGTGACTGGGACGGCTGTGGATGGAAATTCGCCCGCTCAGATGAACTGACCAGGC
  ACTACCGTAAACACACGGGGCACCGCCCGTTCCAGTGCCAAAAATGCGACCGAGCATTTT
  CCAGGTCGGACCACCTCGCCTTACACATGAAGAGGCATTTTTAAATCCCAGACAGTGGAT
  ATGACCCACACTGCCAGAAGAGAATTCAGTATTTTTTACTTTTCACACTGTCTTCCCGAT
  GAGGGAAGGAGCCCAGCCAGAAAGCACTACAATCATGGTCAAGTTCCCAACTGAGTCATC
  TTGTGAGTGGATAATCAGGAAAAATGAGGAATCCAAAAGACAAAAATCAAAGAACAGATG
  GGGTCTGTGACTGGATCTTCTATCATTCCAATTCTAAATCCGACTTGAATATTCCTGGAC
  TTACAAAATGCCAAGGGGGTGACTGGAAGTTGTGGATATCAGGGTATAAATTATATCCGT
  GAGTTGGGGGAGGGAAGACCAGAATTCCCTTGAATTGTGTATTGATGCAATATAAGCATA
  AAAGATCACCTTGTATTCTCTTTACCTTCTAAAAGCCATTATTATGATGTTAGAAGAAGA
  GGAAGAAATTCAGGTACAGAAAACATGTTTAAATAGCCTAAATGATGGTGCTTGGTGAGT
  CTTGGTTCTAAAGGTACCAAACAAGGAAGCCAAAGTTTTCAAACTGCTGCATACTTTGAC
  AAGGAAAATCTATATTTGTCTTCCGATCAACATTTATGACCTAAGTCAGGTAATATACCT
  GGTTTACTTCTTTAGCATTTTTATGCAGACAGTCTGTTATGCACTGTGGTTTCAGATGTG
  CAATAATTTGTACAATGGTTTATTCCCAAGTATGCCTTAAGCAGAACAAATGTGTTTTTC
  TATATAGTTCCTTGCCTTAATAAATATGTAATATAAATTTAAGCAAACGTCTATTTTGTA
  TATTTGTAAACTACAAAGTAAAATGAACATTTTGTGGAGTTTGTATTTTGCATACTCAAG
  GTGAGAATTAAGTTTTAAATAAACCTATAATATTTTATCTGAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004235 unedited
  TTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGACCAGNATAGAA
  CGAACGTGTCTGCGGGCGCGCGGGGAGCAGAGGCGGTGGCGGGCGGCGGCGGCACCGGGA
  GCCGCCGAGTGACCCTCCCCGCCCCTCTGGCCCCCCACCCTCCCACCCGCCCGTGGCCCG
  CGCCCATGGCCGCGCGCGCTCCACACAACTCACCGGAGTCCGCGCCCTGCGCCGCCGACC
  AGTTCGCAGCTCCGCGCCACGGCAGCCAGTCTCACCTGGCGGCACCGCCCGCCCACCGCC
  CCGGCCACAGCCCCTGCGCCCACGGCAGCACTCGAGGCGACCGCGACAGTGGTGGGGGAC
  GCTGCTGAGTGGAAGAGAGCGCAGCCCGGCCACCGGACCTACTTACTCGCCTTGCTGATT
  GTCTATTTTTGCGTTTACAACTTTTCTAAGAACTTTTGTATACAAAGGAACTTTTTAAAA
  AAGACGCTTCCAAGTTATATTTAATCCAAAGAAGAAGGATCTCGGCCAATTTGGGGTTTT
  GGGTTTTGGCTTCGCTTCTTCTCTTACTTGACTTTGGGGTTCAAGTGCCCCACCTGCTTC
  CGGCTGCCCAGGACCCTTCTGGCCCCCACTTATTGAGGCCTCCCCCTGGCGAGTCTGAAT
  TGGCTGCCAGCGACGCACCTGTTCCATATTTCTTCACTTTCGCGTTTGGCCCGCGCGGCA
  GGTACAAAACCCTGCGTCATGCCGTGCCCCGAATTACCCCTGGCGGGAGGACCTTCCCCC
  ATCGAACCAACTTCCCCAAGGCTTTCCGGCCGCCCCTATACCCTCGCGGGGGCGACCCGC
  GGCCCCAACCCTCCAAACCGGTGAGCCCTTTCACCTCTCCGCCCGCACCCC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004235 unedited
  TCTTGGACGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTTCAGAAAAAATTA
  TAGGTTTATTTAAAACTTAATTCTCACCTTGAGTATGCAAAATACAAACTCCACAAAATG
  TTCATTTTACTTTGTAGTTTACAAATATACAAAATAGACGTTTGCTTAAATTTATATTAC
  ATATTTATTAAGGCAAGGAACTATATAGAAAAACACATTTGTTCTGCTTAAGGCATACTT
  GGGAATAAACCATTGTACAAATTATTGCACATCTGAAACCACAGTGCATAACAGACTGTC
  TGCATAAAAATGCTAAAGAAGTAAACCAGGTATATTACCTGACTTAGGTCATAAATGTTG
  ATCGGAAGACAAATATAGATTTTCCTTGTCAAAGTATGCAGCAGTTTGAAAACTTTGGCT
  TCCTTGTTTGGTACCTTTAGAACCAAGACTCACCAAGCACCATCATTTAGGCTATTTAAA
  CATGTTTTCTGTACCTGAATTTCTTCCTCTTCTTCTAACATCATAATAATGGCTTTTAGA
  AGGTAAAGAGAATACAAGGTGATCTTTTATGCTTATATTGCATCAATACACAATTCAAGG
  GAATTCTGGTCTTCCCTCCCCCAACTCACGGATATAATTTATACCCTGATATCCACAACT
  TCCAGTCACCCCCTTGGCATTTTGTAAGTCCAGGAATATTCAAGTCGGATTTAGAATTGG
  AATGATAGAAGATCCAGTCACAGACCCCCATCTGTTCTTTGGATTTTTGGCTTTTGGATT
  CCTCATTTTCCCTGATTATCCACTCCCAAGATGACTCACTTGGGAACTTGACCCTGATTG
  TAATGCTTTCTGCCTGGCTCCTCCCTCATCGGAAAACAGGGGGCAAAGGAAAAAACTGAA
  TCTCTTCCGGCAGGGGGTCCTACCCCC
  
• Restriction Sites: Please inquire
• ACCN: NM_004235
• Insert Size: 2903 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004235.4, NP_004226.3
• RefSeq Size: 2903 bp
• RefSeq ORF: 2949 bp
• Locus ID: 9314
• UniProt ID: O43474
• Cytogenetics: 9q31.2
• Domains: zf-C2H2
• Protein Families: Adult stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors
• Gene Summary: This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]
  Transcript Variant: This variant (2) lacks an in-frame segment in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.