MAT2A (NM_005911) Human Untagged Clone

CAT#: SC324396

MAT2A (untagged)-Human methionine adenosyltransferase II, alpha (MAT2A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAT2A
• Synonyms: MATA2; MATII; SAMS2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005911.4 CGCCCGCCTGCTACGAGTAGAACGCTGTCCGCAGCTTGCGCATTTCGCAGCCGCTGCCGC
  CTCGCCGCTGCTCCTTCGTAAGGCCACTTCCGCACACCGACACCAACATGAACGGACAGC
  TCAACGGCTTCCACGAGGCGTTCATCGAGGAGGGCACATTCCTTTTCACCTCAGAGTCGG
  TCGGGGAAGGCCACCCAGATAAGATTTGTGACCAAATCAGTGATGCTGTCCTTGATGCCC
  ACCTTCAGCAGGATCCTGATGCCAAAGTAGCTTGTGAAACTGTTGCTAAAACTGGAATGA
  TCCTTCTTGCTGGGGAAATTACATCCAGAGCTGCTGTTGACTACCAGAAAGTGGTTCGTG
  AAGCTGTTAAACACATTGGATATGATGATTCTTCCAAAGGTTTTGACTACAAGACTTGTA
  ACGTGCTGGTAGCCTTGGAGCAACAGTCACCAGATATTGCTCAAGGTGTTCATCTTGACA
  GAAATGAAGAAGACATTGGTGCTGGAGACCAGGGCTTAATGTTTGGCTATGCCACTGATG
  AAACTGAGGAGTGTATGCCTTTAACCATTGTCTTGGCACACAAGCTAAATGCCAAACTGG
  CAGAACTACGCCGTAATGGCACTTTGCCTTGGTTACGCCCTGATTCTAAAACTCAAGTTA
  CTGTGCAGTATATGCAGGATCGAGGTGCTGTGCTTCCCATCAGAGTCCACACAATTGTTA
  TATCTGTTCAGCATGATGAAGAGGTTTGTCTTGATGAAATGAGGGATGCCCTAAAGGAGA
  AAGTCATCAAAGCAGTTGTGCCTGCGAAATACCTTGATGAGGATACAATCTACCACCTAC
  AGCCAAGTGGCAGATTTGTTATTGGTGGGCCTCAGGGTGATGCTGGTTTGACTGGACGCA
  AAATCATTGTGGACACTTATGGCGGTTGGGGTGCTCATGGAGGAGGTGCCTTTTCAGGAA
  AGGATTATACCAAGGTCGACCGTTCAGCTGCTTATGCTGCTCGTTGGGTGGCAAAATCCC
  TTGTTAAAGGAGGTCTGTGCCGGAGGGTTCTTGTTCAGGTCTCTTATGCTATTGGAGTTT
  CTCATCCATTATCTATCTCCATTTTCCATTATGGTACCTCTCAGAAGAGTGAGAGAGAGC
  TATTAGAGATTGTGAAGAAGAATTTCGATCTCCGCCCTGGGGTCATTGTCAGGGATCTGG
  ATCTGAAGAAGCCAATTTATCAGAGGACTGCAGCCTATGGCCACTTTGGTAGGGACAGCT
  TCCCATGGGAAGTGCCCAAAAAGCTTAAATATTGAAAGTGTTAGCCTTTTTTCCCCAGAC
  TTGTTGGCGTAGGCTACAGAGAAGCCTTCAAGCTCTGAGGGAAAGGGCCCTCCTTCCTAA
  ATTTTCCTGTCCTCTTTCAGCTCCTGACCAGTTGCAGTCACTCTAGTCAATGACATGAAT
  TTTAGCTTTTGTGGGGGACTGTAAGTTGGGCTTGCTATTCTGTCCCTAGGTGTTTTGTTC
  ACCATTATAATGAATTTAGTGAGCATAGGTGATCCATGTAACTGCCTAGAAACAACACTG
  TAGTAAATAATGCTTTGAAATTGAACCTTTGTGCCCTATCACCCAACGCTCCAAAGTCAT
  AATTGCATTGACTTTCCCCACCAGATGCTGAAAATGTCCTTGTGATGTGCACGTAAAGTA
  CTTGTAGTTCCACTTATAGCCTCTGTCTGGCAATGCCACAGCCCTGTCAGCATGAATTTG
  TAATGTCTTGAGCTCTATTATGAATGTGAAGCCTTCCCCTTATCCTCCCTGTAACTTGAT
  CCATTTCTAATTATGTAGCTCTTTGTCAGGGAGTGTTCCCTATCCAATCAATCTTGCATG
  TAACGCAAGTTCCCAGTTGGAGCTCCAGCCTGACATCAAAAAAGGCAGTTACCATTAAAC
  CATCTCCCTGGTGCTTATGCTCTTAATTGCCACCTCTAACAGCACCAAATCAAAATCTCT
  CCACTTTCAGCTGTCTTTTGGAGGACGTACGTAATAAGGTTTTAATTTAGTAAACCAATC
  CTATGCATGGTTTCAGCACTAGCCAAACCTCACCAACTCCTAGTTCTAGAAAAACAGGCA
  CTTGGCAGCCTTGTGATGTCATACAGAGAAGTCACAGGGCAGTACCTGAGGGTCTGTAGG
  TTGCACACTTTGGTACCAGATAACTTTTTTTTTTCTTTATAAGAAAGCCTGAGTACTCCA
  CACTGCACAATAACTCCTCCCAGGGTTTTAACTTTGTTTTATTTTCAAAACCAGGTCCAA
  TGAGCTTTCTGAACAGCTGGTGTAGCTACAGAGAAACCAGCTTCCTTCAGAGAGCAGTGC
  TTTTGGCGGGGAGGAGGAAATCCCTTCATACTTGAACGTTTTCTAATTGCTTATTTATTG
  TATTCTGGGGTATGGCGTAAGTACAGAGAAGCCATCACCTCAGATGGCAGCTTTTAAAAG
  ATTTTTTTTTTTTCTCTCAACACCATGATTCCTTTAACAACATGTTTCCAGCATTCCCAG
  GTAGGCCAAGGTGTCCTACAGAAAAACCTTGGGTTAGACCTACAGGGGGTCTGGCTGGTG
  TTAACAGAAGGGAGGGCAGAGCTGGTGCGGCTGGCCATGGAGAAAGCTGACTTGGCTGGT
  GTGGTACAGAGAAGCCAGCTTGTTTACATGCTTATTCCATGACTGCTTGCCCTAAGCAGA
  AAGTGCCTTTCAGGATCTATTTTTGGAGGTTTATTACGTATGTCTGGTTCTCAATTCCAA
  CAGTTTAATGAAGATCTAAATAAAATGCTAGGTTCTACCTTAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005911
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005911.4, NP_005902.1
• RefSeq Size: 2835 bp
• RefSeq ORF: 1188 bp
• Locus ID: 4144
• UniProt ID: P31153
• Cytogenetics: 2p11.2
• Domains: S-AdoMet_synt
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways, Selenoamino acid metabolism
• Gene Summary: The protein encoded by this gene catalyzes the production of S-adenosylmethionine (AdoMet) from methionine and ATP. AdoMet is the key methyl donor in cellular processes. [provided by RefSeq, Jun 2011]