MEK1 (MAP2K1) (NM_002755) Human Untagged Clone

CAT#: SC323696

MAP2K1 (untagged)-Kinase deficient mutant (K97M) of Human mitogen-activated protein kinase kinase 1 (MAP2K1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MEK1
• Synonyms: CFC3; MAPKK1; MEK1; MEL; MKK1; PRKMK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323696 sequence for NM_002755 edited (data generated by NextGen Sequencing)
  ATGCCCAAGAAGAAGCCGACGCCCATCCAGCTGAACCCGGCCCCCGACGGCTCTGCAGTT
  AACGGGACCAGCTCTGCGGAGACCAACTTGGAGGCCTTGCAGAAGAAGCTGGAGGAGCTA
  GAGCTTGATGAGCAGCAGCGAAAGCGCCTTGAGGCCTTTCTTACCCAGAAGCAGAAGGTG
  GGAGAACTGAAGGATGACGACTTTGAGAAGATCAGTGAGCTGGGGGCTGGCAATGGCGGT
  GTGGTGTTCAAGGTCTCCCACAAGTCTTCTGGCCTGGTCATGGCCAGAATGCTAATTCAT
  CTGGAGATCAAACCCGCAATCCGGAACCAGATCATAAGGGAGCTGCAGGTTCTGCATGAG
  TGCAACTCTCCGTACATCGTGGGCTTCTATGGTGCGTTCTACAGCGATGGCGAGATCAGT
  ATCTGCATGGAGCACATGGATGGAGGTTCTCTGGATCAAGTCCTGAAGAAAGCTGGAAGA
  ATTCCTGAACAAATTTTAGGAAAAGTTAGCATTGCTGTAATAAAAGGCCTGACATATCTG
  AGGGAGAAGCACAAGATCATGCACAGAGATGTCAAGCCCTCCAACATCCTAGTCAACTCC
  CGTGGGGAGATCAAGCTCTGTGACTTTGGGGTCAGCGGGCAGCTCATCGACTCCATGGCC
  AACTCCTTCGTGGGCACAAGGTCCTACATGTCGCCAGAAAGACTCCAGGGGACTCATTAC
  TCTGTGCAGTCAGACATCTGGAGCATGGGACTGTCTCTGGTAGAGATGGCGGTTGGGAGG
  TATCCCATCCCTCCTCCAGATGCCAAGGAGCTGGAGCTGATGTTTGGGTGCCAGGTGGAA
  GGAGATGCGGCTGAGACCCCACCCAGGCCAAGGACCCCCGGGAGGCCCCTTAGCTCATAC
  GGAATGGACAGCCGACCTCCCATGGCAATTTTTGAGTTGTTGGATTACATAGTCAACGAG
  CCTCCTCCAAAACTGCCCAGTGGAGTGTTCAGTCTGGAATTTCAAGATTTTGTGAATAAA
  TGCTTAATAAAAAACCCCGCAGAGAGAGCAGATTTGAAGCAACTCATGGTTCATGCTTTT
  ATCAAGAGATCTGATGCTGAGGAAGTGGATTTTGCAGGTTGGCTCTGCTCCACCATCGGC
  CTTAACCAGCCCAGCACACCAACCCATGCTGCTGGCGTCTAA
  
  Clone variation with respect to NM_002755.3
  265 c=>t;290 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_002755 unedited
  GCCGCCGTTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGA
  ACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTGAGAGAGAGA
  GAGGAAGGGAATCCCGGGCTGCCGAACCGCACGTTCAGCCCGCTCCGCTCCTGCAGGGCAGCCTTTCGGC
  TCTCTGCGCGCGAAGCCGAGTCCCGGGCGGGTGGGGCGGGGGTCCACTGAGACCGCTACCGGCCCCTCGG
  CGCTGACGGGACCGCGCGGGGGCGCACCCGCTGAAGGCAGCCCCGGGGCCCCGCGGGCCCGGACTTTGGT
  CCTGCGCAGCGGGGCGCGGGGGCAGCGCAGCGGGGAGGAGCCAAGAAGGTGCTGCCCTTCCCCCGGAATT
  GGGAGCCCCGTTACCCGGGTCCAAAAGGCCCAAAAGAAGCCCAACCCCCTTCCACCTGACCCCGGCCCCC
  GCCGGCTTGGCAGTTAACGGGACCAGCTTTCGGGAACCAAACTGGGAGGCCTTGCAAAAAAACCTGGAGG
  GCCTAACTTTGGTGACCACACGAAAAGCCCTTGGGCCTTTTTTACCCAACCAAGGGGGAAAACTGAGGAA
  CACATTTGAAATATCGTGCCGGGCGCGGATGGGGGGTGTGTTAGGTCCCAATTCTGGCCGTGTGGGCCAT
  GTGCATTCTGGATATACCCATCTGGACCATACAGAGACCGCGCTCTGATCGGACACTCCACTAGGGTCTA
  GGGGGGCT
  
  Kinase domain sequence

  >SC323696 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CYCTGMGCAATGGGCGKAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA
  GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTGAGAGAGAGAGAGGAAG
  GGAATCCCGGGCTGCCGAACCGCACGTTCAGCCCGCTCCGCTCCTGCAGGGCAGCCTTTCGGCTCTCTGC
  GCGCGAAGCCGAGTCCCGGGCGGGTGGGGCGGGGGTCCACTGAGA
  
• Restriction Sites: Please inquire
• ACCN: NM_002755
• Insert Size: 2550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002755.2, NP_002746.1
• RefSeq Size: 2222 bp
• RefSeq ORF: 1182 bp
• Locus ID: 5604
• UniProt ID: Q02750
• Cytogenetics: 15q22.31
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, B cell receptor signaling pathway, Bladder cancer, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Dorso-ventral axis formation, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Gap junction, Glioma, GnRH signaling pathway, Insulin signaling pathway, Long-term depression, Long-term potentiation, MAPK signaling pathway, Melanogenesis, Melanoma, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Oocyte meiosis, Pancreatic cancer, Pathways in cancer, Prion diseases, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, T cell receptor signaling pathway, Thyroid cancer, Toll-like receptor signaling pathway, Vascular smooth muscle contraction, VEGF signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq, Jul 2008]