FGR (NM_005248) Human Untagged Clone

CAT#: SC323444

FGR (untagged)-Kinase deficient mutant (K291M) of Human Gardner-Rasheed feline sarcoma viral (v-fgr) oncogene homolog (FGR), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FGR
• Synonyms: c-fgr; c-src2; p55-Fgr; p55c-fgr; p58-Fgr; p58c-fgr; SRC2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323444 sequence for NM_005248 edited (data generated by NextGen Sequencing)
  ATGGGCTGTGTGTTCTGCAAGAAATTGGAGCCGGTGGCCACGGCCAAGGAGGATGCTGGC
  CTGGAAGGGGACTTCAGAAGCTACGGGGCAGCAGACCACTATGGGCCTGACCCCACTAAG
  GCCCGGCCTGCATCCTCATTTGCCCACATCCCCAACTACAGCAACTTCTCCTCTCAGGCC
  ATCAACCCTGGCTTCCTTGATAGTGGCACCATCAGGGGTGTGTCAGGGATTGGGGTGACC
  CTGTTCATTGCCCTGTATGACTATGAGGCTCGAACTGAGGATGACCTCACCTTCACCAAG
  GGCGAGAAGTTCCACATCCTGAACAATACTGAAGGTGACTGGTGGGAGGCTCGGTCTCTC
  AGCTCCGGAAAAACTGGCTGCATTCCCAGCAACTACGTGGCCCCTGTTGACTCAATCCAA
  GCTGAAGAGTGGTACTTTGGAAAGATTGGGAGAAAGGATGCAGAGAGGCAGCTGCTTTCA
  CCAGGCAACCCCCAGGGGGCCTTTCTCATTCGGGAAAGCGAGACCACCAAAGGTGCCTAC
  TCCCTGTCCATCCGGGACTGGGATCAGACCAGAGGCGATCATGTGAAGCATTACAAGATC
  CGCAAACTGGACATGGGCGGCTACTACATCACCACACGGGTTCAGTTCAACTCGGTGCAG
  GAGCTGGTGCAGCACTACATGGAGGTGAATGACGGGCTGTGCAACCTGCTCATCGCGCCC
  TGCACCATCATGAAGCCGCAGACGCTGGGCCTGGCCAAGGACGCCTGGGAGATCAGCCGC
  AGCTCCATCACGCTGGAGCGCCGGCTGGGCACCGGCTGCTTCGGGGATGTGTGGCTGGGC
  ACGTGGAACGGCAGCACTAAGGTGGCGGTGATGACGCTGAAGCCGGGCACCATGTCCCCG
  AAGGCCTTCCTGGAGGAGGCGCAGGTCATGAAGCTGCTGCGGCACGACAAGCTGGTGCAG
  CTGTACGCCGTGGTGTCGGAGGAGCCCATCTACATCGTGACCGAGTTCATGTGTCACGGC
  AGCTTGCTGGATTTTCTCAAGAACCCAGAGGGCCAGGATTTGAGGCTGCCCCAATTGGTG
  GACATGGCAGCCCAGGTAGCTGAGGGCATGGCCTACATGGAACGCATGAACTACATTCAC
  CGCGACCTGAGGGCAGCCAACATCCTGGTTGGGGAGCGGCTGGCGTGCAAGATCGCAGAC
  TTTGGCTTGGCGCGTCTCATCAAGGACGATGAGTACAACCCCTGCCAAGGTTCCAAGTTC
  CCCATCAAGTGGACAGCCCCAGAAGCTGCCCTCTTTGGCAGATTCACCATCAAGTCAGAC
  GTGTGGTCCTTTGGGATCCTGCTCACTGAGCTCATCACCAAGGGCCGAATCCCCTACCCA
  GGCATGAATAAACGGGAAGTGTTGGAACAGGTGGAGCAGGGCTACCACATGCCGTGCCCT
  CCAGGCTGCCCAGCATCCCTGTACGAGGCCATGGAACAGACCTGGCGTCTGGACCCGGAG
  GAGAGGCCTACCTTCGAGTACCTGCAGTCCTTCCTGGAGGACTACTTCACCTCCGCTGAA
  CCACAGTACCAGCCCGGGGATCAGACATAG
  
  Clone variation with respect to NM_005248.2
  872 a=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_005248 unedited
  ACCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAA
  CCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCGGCACTCGCGGC
  TGCTGGAGCCCCGGCTGGCTCACCCCGGGGCCGGGCAGAATTGGGCTCCAGGTCTCTGACCCCTCCCAAG
  GATCATGCCGCAGCCCCACTGACCCAGGAGTAGGGGCCTAAGGGCAGGGAACCTGGAATGGGCTGTGTGT
  TCTGCAAGAAATTGGAGCCGGTGGCCACGGCCAAGGAGGATGCTGGCCTGGAAGGGACTTCAGAAAGCTA
  CGGGGCAGCAGACCACTATGGGCCTGACCCCCACTAAGGCCCGGCCTGCATCCCTCATTTGCCCCACATC
  CCCAACTACAGCAACTTCTCCTCTCAGGCCATCAACCCTGCTTCCTTGATAGTGGCACCATCAGGGTGTG
  TCAGGATGGGGTGACCTGGTCATTGCCTGTGACTTTAAGCTCGAACTAAGAGACTACTTCCCCAAGGCAA
  AAGTTCCATCCTGACATCCTGAGGAACTGTGAGCTCGTTCTCACTCGAAAACTGCTCATTCCAGCACACT
  GCCCTGTTGACTATCCACCTAAAAGTGACTTGAAATTGGAAAGGACGAAAGCATCTTTCACGACCAGGGC
  CTTACTCGGACAACCAGGCTTCGGTCTGTGTGTAAAAAA
  
  Kinase domain sequence

  >SC323444 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CKGCRCGCGGCTGGGCACGGCTGCTTCGGGGATGTGTGGCTGGGCACGTGGAACGGCAGCACTAAGGTGG
  CGGTGATGACGCTGAAGCCGGGCACCATGTCCCCGAAGGCCTTCCTGGAGGAGGCGCAGGTCATGAAGCT
  GCTGCGGCACGACAAGCTGGTGCAGCTGTACGCCGTGGTGTCGGAGGAGCCCATCTACATCGTGACCGAG
  TTCATGTGTCACGGCAGCTTGCTGGATTTTCTCAAGAACCCAGAG
  
• Restriction Sites: Please inquire
• ACCN: NM_005248
• Insert Size: 2450 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005248.1, NP_005239.1
• RefSeq Size: 2354 bp
• RefSeq ORF: 1590 bp
• Locus ID: 2268
• UniProt ID: P09769
• Cytogenetics: 1p35.3
• Domains: pkinase, SH2, TyrKc, SH3, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway
• Gene Summary: This gene is a member of the Src family of protein tyrosine kinases (PTKs). The encoded protein contains N-terminal sites for myristylation and palmitylation, a PTK domain, and SH2 and SH3 domains which are involved in mediating protein-protein interactions with phosphotyrosine-containing and proline-rich motifs, respectively. The protein localizes to plasma membrane ruffles, and functions as a negative regulator of cell migration and adhesion triggered by the beta-2 integrin signal transduction pathway. Infection with Epstein-Barr virus results in the overexpression of this gene. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) initiates at exon M4 and represents the longest transcript. It is expressed in myelomonocytic cells. Variants 1, 2, and 3 encode the same isoform.