P70 S6 Kinase beta (RPS6KB2) (NM_003952) Human Untagged Clone

CAT#: SC319382

RPS6KB2 (untagged)-Human ribosomal protein S6 kinase, 70kDa, polypeptide 2 (RPS6KB2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: P70 S6 Kinase beta
• Synonyms: KLS; p70(S6K)-beta; P70-beta; P70-beta-1; P70-beta-2; p70S6Kb; S6K-beta2; S6K2; S6KB; S6Kbeta; S6KI(2); SRK; STK14B
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003952.2 GTCAGTCAGTGCGCGGCCAGGTACGGGCCGACGGGCCCGCGGGGCCGGCGCCGCCATGGC
  GGCCGTGTTTGATTTGGATTTGGAGACGGAGGAAGGCAGCGAGGGCGAGGGCGAGCCAGA
  GCTCAGCCCCGCGGACGCATGTCCCCTTGCCGAGTTGAGGGCAGCTGGCCTAGAGCCTGT
  GGGACACTATGAAGAGGTGGAGCTGACTGAGACCAGCGTGAACGTTGGCCCAGAGCGCAT
  CGGGCCCCACTGCTTTGAGCTGCTGCGTGTGCTGGGCAAGGGGGGCTATGGCAAGGTGTT
  CCAGGTGCGAAAGGTGCAAGGCACCAACTTGGGCAAAATATATGCCATGAAAGTCCTAAG
  GAAGGCCAAAATTGTGCGCAATGCCAAGGACACAGCACACACACGGGCTGAGCGGAACAT
  TCTAGAGTCAGTGAAGCACCCCTTTATTGTGGAACTGGCCTATGCCTTCCAGACTGGTGG
  CAAACTCTACCTCATCCTTGAGTGCCTCAGTGGTGGCGAGCTCTTCACGCATCTGGAGCG
  AGAGGGCATCTTCCTGGAAGATACGGCCTGCTTCTACCTGGCTGAGATCACGCTGGCCCT
  GGGCCATCTCCACTCCCAGGGCATCATCTACCGGGACCTCAAGCCCGAGAACATCATGCT
  CAGCAGCCAGGGCCACATCAAACTGACCGACTTTGGACTCTGCAAGGAGTCTATCCATGA
  GGGCGCCGTCACTCACACCTTCTGCGGCACCATTGAGTACATGGCCCCTGAGATTCTGGT
  GCGCAGTGGCCACAACCGGGCTGTGGACTGGTGGAGCCTGGGGGCCCTGATGTACGACAT
  GCTCACTGGATCGCCGCCCTTTACCGCAGAGAACCGGAAGAAAACCATGGATAAGATCAT
  CAGGGGCAAGCTGGCACTGCCCCCCTACCTCACCCCAGATGCCCGGGACCTTGTCAAAAA
  GTTTCTGAAACGGAATCCCAGCCAGCGGATTGGGGGTGGCCCAGGGGATGCTGCTGATGT
  GCAGAGACATCCCTTTTTCCGGCACATGAATTGGGACGACCTTCTGGCCTGGCGTGTGGA
  CCCCCCTTTCAGGCCCTGTCTGCAGTCAGAGGAGGACGTGAGCCAGTTTGATACCCGCTT
  CACACGGCAGACGCCGGTGGACAGTCCTGATGACACAGCCCTCAGCGAGAGTGCCAACCA
  GGCCTTCCTGGGCTTCACATACGTGGCGCCGTCTGTCCTGGACAGCATCAAGGAGGGCTT
  CTCCTTCCAGCCCAAGCTGCGCTCACCCAGGCGCCTCAACAGTAGCCCCCGGGTCCCCGT
  CAGCCCCCTCAAGTTCTCCCCTTTTGAGGGGTTTCGGCCCAGCCCCAGCCTGCCGGAGCC
  CACGGAGCTACCTCTACCTCCACTCCTGCCACCGCCGCCGCCCTCGACCACCGCCCCTCT
  CCCCATCCGTCCCCCCTCAGGGACCAAGAAGTCCAAGAGGGGCCGTGGGCGTCCAGGGCG
  CTAGGAAGCCGGGTGGGGGTGAGGGTAGCCCTTGAGCCCTGTCCCTGCGGCTGTGAGAGC
  AGCAGGACCCTGGGCCAGTTCCAGAGACCTGGGGGTGTGTCTGGGGGTGGGGTGTGAGTG
  CGTATGAAAGTGTGTGTCTGCTGGGGCAGCTGTGCCCCTGAATCATGGGCACGGAGGGCC
  GCCCGCCACACCCCGCGCTCAACTGCTCCCGTGGAAGATTAAAGGGCTGAATCATGAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: EcoRI-XhoI
• ACCN: NM_003952
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003952.2, NP_003943.2
• RefSeq Size: 1782 bp
• RefSeq ORF: 1449 bp
• Locus ID: 6199
• UniProt ID: Q9UBS0
• Cytogenetics: 11q13.2
• Domains: pkinase, S_TK_X, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Acute myeloid leukemia, ErbB signaling pathway, Fc gamma R-mediated phagocytosis, Insulin signaling pathway, mTOR signaling pathway, TGF-beta signaling pathway
• Gene Summary: This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains a kinase catalytic domain and phosphorylates the S6 ribosomal protein and eukaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation. [provided by RefSeq, Jan 2015]