DNMT3L (NM_013369) Human Untagged Clone

CAT#: SC319376

DNMT3L (untagged)-Human DNA (cytosine-5-)-methyltransferase 3-like (DNMT3L), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNMT3L
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_013369.2 GGCACGAGGGTGGGCCTGGATCCTTCCAGCTCATTCTTTGCCTGCGCCGTCCCTCGTTCC
  ATGGCCCAGTCCTCCCCGGGGACCCTGAGCCTGGAAGCCCCGGACCACTGGAACCTTGAA
  CCCACCAGCTGGCTGTACCCGGAGCCGTGGCAGCAGCCCTCATCCCCATGGCGGCCATCC
  CAGCCCTGGACCCAGAGGCCGAGCCCAGCATGGACGTGATTTTGGTGGGATCCAGTGAGC
  TCTCAAGCTCCGTTTCACCCGGGACAGGCAGAGATCTTATTGCATATGAAGTCAAGGCTA
  ACCAGCGAAATATAGAAGACATCTGCATCTGCTGCGGAAGTCTCCAGGTTCACACACAGC
  ACCCTCTGTTTGAGGGAGGGATCTGCGCCCCATGTAAGGACAAGTTCCTGGATGCCCTCT
  TCCTGTACGACGATGACGGGTACCAATCCTACTGCTCCATCTGCTGCTCCGGAGAGACGC
  TGCTCATCTGCGGAAACCCTGATTGCACCCGATGCTACTGCTTCGAGTGTGTGGATAGCC
  TGGTCGGCCCCGGGACCTCGGGGAAGGTGCACGCCATGAGCAACTGGGTGTGCTACCTGT
  GCCTGCCGTCCTCCCGAAGCGGGCTGCTGCAGCGTCGGAGGAAGTGGCGCAGCCAGCTCA
  AGGCCTTCTACGACCGAGAGTCGGAGAATCCCCTTGAGATGTTCGAAACCGTGCCTGTGT
  GGAGGAGACAGCCAGTCCGGGTGCTGTCCCTTTTTGAAGACATCAAGAAAGAGCTGACGA
  GTTTGGGCTTTTTGGAAAGTGGTTCTGACCCGGGACAACTGAAGCATGTGGTTGATGTCA
  CAGACACAGTGAGGAAGGATGTGGAGGAGTGGGGACCCTTCGATCTTGTGTACGGCGCCA
  CACCTCCCCTGGGCCACACCTGTGACCGTCCTCCCAGCTGGTACCTGTTCCAGTTCCACC
  GGCTCCTGCAGTACGCACGGCCCAAGCCAGGCAGCCCCGGGCCCTTCTTCTGGATGTTCG
  TGGACAATCTGGTGCTGAACAAGGAAGACCTGGACGTCGCATCTCGCTTCCTGGAGATGG
  AGCCAGTCACCATCCCAGATGTCCACGGCGGATCCTTGCAGAATGCTGTCCGCGTGTGGA
  GCAACATCCCAGCCATAAGGAGCAGGCACTGGGCTCTGGTTTCGGAAGAAGAATTGTCCC
  TGCTGGCCCAGAACAAGCAGAGCTCGAAGCTCGCGGCCAAGTGGCCCACCAAGCTGGTGA
  AGAACTGCTTTCTCCCCCTAAGAGAATATTTCAAGTATTTTTCAACAGAACTCACTTCCT
  CTTTATAAATGAGTCACTATACTGTGAAGAAAAAGACTTTTCCTAGAACAAAGAAAAAAA
  AAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_013369
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013369.2, NP_037501.2
• RefSeq Size: 1705 bp
• RefSeq ORF: 1164 bp
• Locus ID: 29947
• UniProt ID: Q9UJW3
• Cytogenetics: 21q22.3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• Gene Summary: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases, but is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, it does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]
  Transcript Variant: This variant (1) encodes the longer isoform (1).