ErbB 4 (ERBB4) (NM_001042599) Human Untagged Clone

CAT#: SC311428

ERBB4 (untagged)-Human v-erb-a erythroblastic leukemia viral oncogene homolog 4 (avian) (ERBB4), transcript variant JM-a/CVT-2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ErbB 4
• Synonyms: ALS19; HER4; p180erbB4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001042599 edited
  TCAGCACGGGATCTGAGACTTCCAAAAAATGAAGCCGGCGACAGGACTTTGGGTCTGGGT
  GAGCCTTCTCGTGGCGGCGGGGACCGTCCAGCCCAGCGATTCTCAGTCAGTGTGTGCAGG
  AACGGAGAATAAACTGAGCTCTCTCTCTGACCTGGAACAGCAGTACCGAGCCTTGCGCAA
  GTACTATGAAAACTGTGAGGTTGTCATGGGCAACCTGGAGATAACCAGCATTGAGCACAA
  CCGGGACCTCTCCTTCCTGCGGTCTGTTCGAGAAGTCACAGGCTACGTGTTAGTGGCTCT
  TAATCAGTTTCGTTACCTGCCTCTGGAGAATTTACGCATTATTCGTGGGACAAAACTTTA
  TGAGGATCGATATGCCTTGGCAATATTTTTAAACTACAGAAAAGATGGAAACTTTGGACT
  TCAAGAACTTGGATTAAAGAACTTGACAGAAATCCTAAATGGTGGAGTCTATGTAGACCA
  GAACAAATTCCTTTGTTATGCAGACACCATTCATTGGCAAGATATTGTTCGGAACCCATG
  GCCTTCCAACTTGACTCTTGTGTCAACAAATGGTAGTTCAGGATGTGGACGTTGCCATAA
  GTCCTGTACTGGCCGTTGCTGGGGACCCACAGAAAATCATTGCCAGACTTTGACAAGGAC
  GGTGTGTGCAGAACAATGTGACGGCAGATGCTACGGACCTTACGTCAGTGACTGCTGCCA
  TCGAGAATGTGCTGGAGGCTGCTCAGGACCTAAGGACACAGACTGCTTTGCCTGCATGAA
  TTTCAATGACAGTGGAGCATGTGTTACTCAGTGTCCCCAAACCTTTGTCTACAATCCAAC
  CACCTTTCAACTGGAGCACAATTTCAATGCAAAGTACACATATGGAGCATTCTGTGTCAA
  GAAATGTCCACATAACTTTGTGGTAGATTCCAGTTCTTGTGTGCGTGCCTGCCCTAGTTC
  CAAGATGGAAGTAGAAGAAAATGGGATTAAAATGTGTAAACCTTGCACTGACATTTGCCC
  AAAAGCTTGTGATGGCATTGGCACAGGATCATTGATGTCAGCTCAGACTGTGGATTCCAG
  TAACATTGACAAATTCATAAACTGTACCAAGATCAATGGGAATTTGATCTTTCTAGTCAC
  TGGTATTCATGGGGACCCTTACAATGCAATTGAAGCCATAGACCCAGAGAAACTGAACGT
  CTTTCGGACAGTCAGAGAGATAACAGGTTTCCTGAACATACAGTCATGGCCACCAAACAT
  GACTGACTTCAGTGTTTTTTCTAACCTGGTGACCATTGGTGGAAGAGTACTCTATAGTGG
  CCTGTCCTTGCTTATCCTCAAGCAACAGGGCATCACCTCTCTACAGTTCCAGTCCCTGAA
  GGAAATCAGCGCAGGAAACATCTATATTACTGACAACAGCAACCTGTGTTATTATCATAC
  CATTAACTGGACAACACTCTTCAGCACAATCAACCAGAGAATAGTAATCCGGGACAACAG
  AAAAGCTGAAAATTGTACTGCTGAAGGAATGGTGTGCAACCATCTGTGTTCCAGTGATGG
  CTGTTGGGGACCTGGGCCAGACCAATGTCTGTCGTGTCGCCGCTTCAGTAGAGGAAGGAT
  CTGCATAGAGTCTTGTAACCTCTATGATGGTGAATTTCGGGAGTTTGAGAATGGCTCCAT
  CTGTGTGGAGTGTGACCCCCAGTGTGAGAAGATGGAAGATGGCCTCCTCACATGCCATGG
  ACCGGGTCCTGACAACTGTACAAAGTGCTCTCATTTTAAAGATGGCCCAAACTGTGTGGA
  AAAATGTCCAGATGGCTTACAGGGGGCAAACAGTTTCATTTTCAAGTATGCTGATCCAGA
  TCGGGAGTGCCACCCATGCCATCCAAACTGCACCCAAGGGTGTAACGGTCCCACTAGTCA
  TGACTGCATTTACTACCCATGGACGGGCCATTCCACTTTACCACAACATGCTAGAACTCC
  CCTGATTGCAGCTGGAGTAATTGGTGGGCTCTTCATTCTGGTCATTGTGGGTCTGACATT
  TGCTGTTTATGTTAGAAGGAAGAGCATCAAAAAGAAAAGAGCCTTGAGAAGATTCTTGGA
  AACAGAGTTGGTGGAACCATTAACTCCCAGTGGCACAGCACCCAATCAAGCTCAACTTCG
  TATTTTGAAAGAAACTGAGCTGAAGAGGGTAAAAGTCCTTGGCTCAGGTGCTTTTGGAAC
  GGTTTATAAAGGTATTTGGGTACCTGAAGGAGAAACTGTGAAGATTCCTGTGGCTATTAA
  GATTCTTAATGAGACAACTGGTCCCAAGGCAAATGTGGAGTTCATGGATGAAGCTCTGAT
  CATGGCAAGTATGGATCATCCACACCTAGTCCGGTTGCTGGGTGTGTGTCTGAGCCCAAC
  CATCCAGCTGGTTACTCAACTTATGCCCCATGGCTGCCTGTTGGAGTATGTCCACGAGCA
  CAAGGATAACATTGGATCACAACTGCTGCTTAACTGGTGTGTCCAGATAGCTAAGGGAAT
  GATGTACCTGGAAGAAAGACGACTCGTTCATCGGGATTTGGCAGCCCGTAATGTCTTAGT
  GAAATCTCCAAACCATGTGAAAATCACAGATTTTGGGCTAGCCAGACTCTTGGAAGGAGA
  TGAAAAAGAGTACAATGCTGATGGAGGAAAGATGCCAATTAAATGGATGGCTCTGGAGTG
  TATACATTACAGGAAATTCACCCATCAGAGTGACGTTTGGAGCTATGGAGTTACTATATG
  GGAACTGATGACCTTTGGAGGAAAACCCTATGATGGAATTCCAACGCGAGAAATCCCTGA
  TTTATTAGAGAAAGGAGAACGTTTGCCTCAGCCTCCCATCTGCACTATTGACGTTTACAT
  GGTCATGGTCAAATGTTGGATGATTGATGCTGACAGTAGACCTAAATTTAAGGAACTGGC
  TGCTGAGTTTTCAAGGATGGCTCGAGACCCTCAAAGATACCTAGTTATTCAGGGTGATGA
  TCGTATGAAGCTTCCCAGTCCAAATGACAGCAAGTTCTTTCAGAATCTCTTGGATGAAGA
  GGATTTGGAAGATATGATGGATGCTGAGGAGTACTTGGTCCCTCAGGCTTTCAACATCCC
  ACCTCCCATCTATACTTCCAGAGCAAGAATTGACTCGAATAGGAACCAGTTTGTATACCG
  AGATGGAGGTTTTGCTGCTGAACAAGGAGTGTCTGTGCCCTACAGAGCCCCAACTAGCAC
  AATTCCAGAAGCTCCTGTGGCACAGGGTGCTACTGCTGAGATTTTTGATGACTCCTGCTG
  TAATGGCACCCTACGCAAGCCAGTGGCACCCCATGTCCAAGAGGACAGTAGCACCCAGAG
  GTACAGTGCTGACCCCACCGTGTTTGCCCCAGAACGGAGCCCACGAGGAGAGCTGGATGA
  GGAAGGTTACATGACTCCTATGCGAGACAAACCCAAACAAGAATACCTGAATCCAGTGGA
  GGAGAACCCTTTTGTTTCTCGGAGAAAAAATGGAGACCTTCAAGCATTGGATAATCCCGA
  ATATCACAATGCATCCAATGGTCCACCCAAGGCCGAGGATGAGTATGTGAATGAGCCACT
  GTACCTCAACACCTTTGCCAACACCTTGGGAAAAGCTGAGTACCTGAAGAACAACATACT
  GTCAATGCCAGAGAAGGCCAAGAAAGCGTTTGACAACCCTGACTACTGGAACCACAGCCT
  GCCACCTCGGAGCACCCTTCAGCACCCAGACTACCTGCAGGAGTACAGCACAAAATATTT
  TTATAAACAGAATGGGCGGATCCGGCCTATTGTGGCAGAGAATCCTGAATACCTCTCTGA
  GTTCTCCCTGAAGCCAGGCACTGTGCTGCCGCCTCCACCTTACAGACACCGGAATACTGT
  GGTGTAAGCTCAGTTGTGGTTTTTTAGGTGGAGAGACACACCTGCTCCAATTTCCCCACC
  CCCCTCTCTTTCTCTGGTGGTCTTCCTTCTACCCCAAGGCCAGTAGTTTTGACACTTCCC
  AGTGGAAGATACAGAGATGCAATGATAGTTATGTGCTTACCTAACTTGAACATTAGAGGG
  AAAGACTGAAAGAGAAAGATAGGAGGAACCACAATGTTTCTTCATTTCTCTGCATGGGTT
  GGTCAGGAGAATGAAACAGCTAGAGAAGGACCAGAAAATGTAAGGCAATGCTGCCTACTA
  TCAAACTAGCTGTCACTTTTTTTCTTTTTCTTTTTCTTTCTTTGTTTCTTTCTTCCTCTT
  CTTTTTTTTTTTTTTTTTTAAAGCAGATGGTTGAAACACCCATGCTATCTGTTCCTATCT
  GCAGGAACTGATGTGTGCATATTTAGCATCCCTGGAAATCATAATAAAGTTTCCATTAGA
  ACAAAAGAATAACATTTTCTATAACATATGATGGTGTCTGAAATTGAGAATCCAGTTTCT
  TTCCCCAGCAGTTTCTGTCCTAGCAAGTAAGAATGGCCAACTCAACTTTCATAATTTAAA
  AATCTCCATTAAAGTTATAACTAGTAATTATGTTTTCAACACTTTTTGGTTTTTTTCATT
  TTGTTTTGCTCTGACCGATTCCTTTATATTTGCTCCCCTATTTTTGGCTTTAATTTCTAA
  TTGCAAAGATGTTTACATCAAAGCTTCTTCACAGAATTTAAGCAAGAAATATTTTAATAT
  AGTGAAATGGCCACTACTTTAAGTATACAATCTTTAAAATAAGAAAGGGAGGCTAATATT
  TTTCATGCTATCAAATTATCTTCACCCTCATCCTTTACATTTTTCAACATTTTTTTTTCT
  CCATAAATGACACTACTTGATAGGCCGTTGGTTGTCTGAAGAGTAGAAGGGAAACTAAGA
  GACAGTTCTCTGTGGTTCAGGAAAACTACTGATACTTTCAGGGGTGGCCCAATGAGGGAA
  TCCATTGAACTGGAAGAAACACACTGGATTGGGTATGTCTACCTGGCAGATACTCAGAAA
  TGTAGTTTGCACTTAAGCTGTAATTTTATTTGTTCTTTTTCTGAACTCCATTTTGGATTT
  TGAATCAAGCAATATGGAAGCAACCAGCAAATTAACTAATTTAAGTACATTTTTAAAAAA
  AGAGCTAAGATAAAGACTGTGGAAATGCCAAACCAAGCAAATTAGGAACCTTGCAACGGT
  ATCCAGGGACTATGATGAGAGGCCAGCACATTATCTTCATATGTCACCTTTGCTACGCAA
  GGAAATTTGTTCAGTTCGTATACTTCGTAAGAAGGAATGCGAGTAAGGATTGGCTTGAAT
  TCCATGGAATTTCTAGTATGAGACTATTTATATGAAGTAGAAGGTAACTCTTTGCACATA
  AATTGGTATAATAAAAAGAAAAACACAAACATTCAAAGCTTAGGGATAGGTCCTTGGGTC
  AAAAGTTGTAAATAAATGTGAAACATCTTCTCATGCAATTATTTTATTATCCAACACACT
  AATCTTTTGATACTTTATATAATTCCCTTTCTTCATATACTGCATCCAGTACTAGAACCA
  TCATTATTATGTATCATTTTGAAAGAATACCTGATGAGATGAAGGATGAGAACAAATGAC
  AGAGATGAGTCTCCAAGTAAAGGGGGCCTCACATCAATAATTAGGAAACTTAGATATAAG
  TCGCCCTTTTCTGAAAATTCTACCCCAAGTCATTTAGATTTTTAAAAAATATTTCTAATG
  TTAAAATATTGGGACCAAATTAGAATCAATAGTATAAGATTAATTAATTAGAGTAAAAAT
  ATCTATTAAGGCAGAGAAAGTTTAGAGAAAAAAATCCAAAGAAATTTGTGTTTCTTCCTA
  TTCTGAACAAGTAAATCCATCCATCCATCCATCCAAACCTCCTTTATCTAACTGTGTCTA
  CTAAAAGCACCATGTTTTGTGGGGAACACTCAGATAAATGGAATATCATCCTCAACTTCA
  AAATTCTATGATCTAGGAGATTTAATTAAAATGACATTTTAATTTTTCTATGCGTTCCAA
  CAATCAGATTGCATAGTCTCTTTTGTGAATAGCTGTCATATAATCAGTTGTACTGTAAGA
  TATCTCCTTTAAACTCATTTGGGATATAAGTTAAACATCCTTCAAATTGTTGATGTTGAC
  AAACAGGATAATTTCAATAATATTATTCAAACATAAACTGGTCTAGGAGAATATTGCATC
  ACTGACTAATTAGCCTATCTAGA
  
• Restriction Sites: Please inquire
• ACCN: NM_001042599
• Insert Size: 6200 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001042599.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042599.1, NP_001036064.1
• RefSeq Size: 11893 bp
• RefSeq ORF: 3879 bp
• Locus ID: 2066
• UniProt ID: Q15303
• Cytogenetics: 2q34
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Calcium signaling pathway, Endocytosis, ErbB signaling pathway
• Gene Summary: This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (JM-a/CYT-2) lacks an alternate in-frame exon, compared to variant JM-a/CYT-1, resulting in a shorter protein (isoform JM-a/CYT-2) that lacks the phosphotidylinositol-3 kinase binding site, compared to isoform JM-a/CYT-1.