MSK1 (RPS6KA5) (NM_182398) Human Untagged Clone

CAT#: SC128215

RPS6KA5 (untagged)-Human ribosomal protein S6 kinase, 90kDa, polypeptide 5 (RPS6KA5), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MSK1
• Synonyms: MSK1; MSPK1; RLPK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC128215 sequence for NM_182398 edited (data generated by NextGen Sequencing)
  ATGGAGGAGGAGGGTGGCAGCAGCGGCGGCGCCGCGGGGACCAGCGCGGACGGCGGCGAC
  GGAGGAGAGCAGCTCCTCACTGTCAAGCACGAGCTGCGGACTGCTAATTTGACAGGACAT
  GCTGAGAAGGTGGGAATAGAAAATTTTGAGCTCCTGAAGGTCCTAGGAACTGGAGCTTAT
  GGAAAAGTATTTCTAGTTCGTAAAATAAGTGGCCATGATACTGGAAAGCTGTATGCCATG
  AAAGTTTTGAAAAAGGCAACAATCGTTCAAAAGGCCAAAACCACAGAGCATACAAGGACA
  GAACGACAAGTCCTGGAACACATTAGGCAGTCGCCATTTTTGGTAACATTACATTATGCT
  TTCCAGACAGAAACCAAACTTCATCTCATTTTAGATTATATAAATGGTGGTGAACTTTTT
  ACTCATCTTTCTCAAAGAGAGCGTTTCACAGAGCATGAGGTGCAGATTTATGTTGGAGAG
  ATTGTGCTTGCCCTCGAACATCTCCACAAGTTGGGGATTATATATCGTGATATTAAGCTT
  GAGAATATTCTACTTGATTCTAATGGCCATGTGGTGCTGACAGATTTTGGTCTGAGTAAG
  GAGTTTGTGGCTGATGAAACTGAAAGAGCATATTCCTTTTGTGGAACTATTGAATACATG
  GCACCAGATATTGTCAGAGGGGGAGATTCAGGACATGACAAGGCAGTTGACTGGTGGAGT
  TTGGGTGTTCTAATGTATGAATTACTAACTGGAGCATCTCCTTTCACTGTTGATGGAGAA
  AAAAATTCCCAAGCTGAGATATCTAGGAGAATATTAAAAAGTGAGCCTCCATATCCCCAA
  GAAATGAGTGCTTTAGCGAAAGACCTAATTCAGCGTCTTTTGATGAAAGATCCCAAGAAG
  AGATTGGGATGTGGTCCACGTGATGCAGATGAAATCAAAGAACATCTCTTCTTTCAGAAA
  ATAAATTGGGATGATTTAGCCGCCAAAAAAGTGCCTGCACCATTTAAGCCAGTCATTCGA
  GATGAATTAGATGTGAGTAACTTTGCAGAAGAGTTCACAGAAATGGATCCCACTTATTCT
  CCCGCAGCCCTGCCCCAGAGTTCTGAGAAGCTGTTTCAGGGCTATTCCTTTGTTGCTCCT
  TCCATCCTATTCAAGCGTAATGCAGCTGTCATAGACCCTCTTCAGTTTCACATGGGAGTT
  GAACGTCCTGGAGTGACAAATGTTGCCAGGAGTGCAATGATGAAGGACTCTCCATTCTAT
  CAACACTATGACCTAGATTTGAAGGACAAACCCCTGGGAGAAGGTAGTTTTTCAATTTGT
  CGAAAGTGTGTGCATAAAAAAAGTAACCAAGCTTTTGCAGTCAAAATAATCAGCAAAAGG
  ATGGAAGCCAATACTCAAAAGGAAATAACAGCTCTGAAACTCTGTGAAGGACACCCCAAT
  ATTGTGAAGTTGCATGAAGTTTTTCATGATCAGCTTCACACGTTTCTAGTGATGGAACTT
  CTGAATGGAGGAGAACTGTTTGAGCGCATTAAGAAAAAGAAGCACTTCAGTGAGACGGAA
  GCCAGCTACATCATGAGGAAGCTTGTTTCAGCTGTAAGCCACATGCATGATGTTGGAGTG
  GTGCACAGGGATCTGAAACCTGAGGTATAA
  
  Clone variation with respect to NM_182398.1
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_182398 unedited
  NNNNNNGGGGAAGTTCAGAATTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACG
  AGGCGAGCCGTGCGGCCAGACGGGAAAGAACTCGTCTTTGCGTCCGAGTTCTGGAGCCGC
  CGCACCCCGACTCCTGGGGCCGCGGCAGCGGCTGCGAGGGGACGGGCGTCCGCTGTCTCC
  TGGGTTCCCCTCGTAGCGACCCGCGGGATCGGAAAAAAAGGAGAAGATGGAGGAGGAGGG
  TGGCAGCAGCGGCGGCGCCGCGGGGACCAGCGCGGACGGCGGCGACGGAGGAGAGCAGCT
  CCTCACTGTCAAGCACGAGCTGCGGACTGCTAATTTGACAGGACATGCTGAGAAGGTGGG
  AATAGAAAATTTTGAGCTCCTGAAGGTCCTAGGAACTGGAGCTTATGGAAAAGTATTTCT
  AGTTCGTAAAATAAGTGGCCATGATACTGGAAAGCTGTATGCCATGAAAGTTTTGAAAAA
  GGCAACAATCGTTCAAAAGGCCAAAACCACAGAGCATACAAGGACAGAACGACAAGTCCT
  GGAACACATTAGGCAGTCGCCATTTTTGGTAACATTACATTATGCTTTCCAGACAGAAAC
  CAAACTTCATCTCATTTTAGATTATATAAATGGTGGTGAACTTTTTACTCATCTTTCTCA
  AAGAGAGCGTTTCACAGAGCATGAGGTGCAGATTTATGTTGGAGAGATTGTGCTTGCCCT
  CGAACATCTCCACAAGTTGGGGATTATATATCGTGATATTAAGCTTGAGAATATTCTACT
  TGATTCTAATGGCCATGTGGTGCTGACAGATTTTGGTCTGAGTAAAGAGTTTGTGGCTGA
  TGAAACTGAAAGAGCATATTCCTTTTGTGGAACT
  
• Restriction Sites: Please inquire
• ACCN: NM_182398
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182398.1, NP_872198.1
• RefSeq Size: 2343 bp
• RefSeq ORF: 1650 bp
• Locus ID: 9252
• UniProt ID: O75582
• Cytogenetics: 14q32.11
• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Bladder cancer, MAPK signaling pathway, Neurotrophin signaling pathway
• Gene Summary: Serine/threonine-protein kinase that is required for the mitogen or stress-induced phosphorylation of the transcription factors CREB1 and ATF1 and for the regulation of the transcription factors RELA, STAT3 and ETV1/ER81, and that contributes to gene activation by histone phosphorylation and functions in the regulation of inflammatory genes (PubMed:11909979, PubMed:12569367, PubMed:12763138, PubMed:9687510, PubMed:18511904, PubMed:9873047). Phosphorylates CREB1 and ATF1 in response to mitogenic or stress stimuli such as UV-C irradiation, epidermal growth factor (EGF) and anisomycin (PubMed:11909979, PubMed:9873047). Plays an essential role in the control of RELA transcriptional activity in response to TNF and upon glucocorticoid, associates in the cytoplasm with the glucocorticoid receptor NR3C1 and contributes to RELA inhibition and repression of inflammatory gene expression (PubMed:12628924, PubMed:18511904). In skeletal myoblasts is required for phosphorylation of RELA at 'Ser-276' during oxidative stress (PubMed:12628924). In erythropoietin-stimulated cells, is necessary for the 'Ser-727' phosphorylation of STAT3 and regulation of its transcriptional potential (PubMed:12763138). Phosphorylates ETV1/ER81 at 'Ser-191' and 'Ser-216', and thereby regulates its ability to stimulate transcription, which may be important during development and breast tumor formation (PubMed:12569367). Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A (PubMed:15010469). Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and EGF, which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN (PubMed:12773393). May also phosphorylate 'Ser-28' of histone H3 (PubMed:12773393). Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 1 (HMGN1/HMG14) (PubMed:12773393). In lipopolysaccharide-stimulated primary macrophages, acts downstream of the Toll-like receptor TLR4 to limit the production of pro-inflammatory cytokines (By similarity). Functions probably by inducing transcription of the MAP kinase phosphatase DUSP1 and the anti-inflammatory cytokine interleukin 10 (IL10), via CREB1 and ATF1 transcription factors (By similarity). Plays a role in neuronal cell death by mediating the downstream effects of excitotoxic injury (By similarity). Phosphorylates TRIM7 at 'Ser-107' in response to growth factor signaling via the MEK/ERK pathway, thereby stimulating its ubiquitin ligase activity (PubMed:25851810).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (2) contains an alternate in-frame segment in the 3' coding region, compared to variant 1. Isoform b has a shorter C-terminus, compared to isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.