APJ Receptor (APLNR) (NM_005161) Human Untagged Clone

CAT#: SC125334

APLNR (untagged)-Human apelin receptor (APLNR), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: APJ Receptor
• Synonyms: AGTRL1; APJ; APJR; HG11
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC125334 sequence for NM_005161 edited (data generated by NextGen Sequencing)
  ATGGAGGAAGGTGGTGATTTTGACAACTACTATGGGGCAGACAACCAGTCTGAGTGTGAG
  TACACAGACTGGAAATCCTCGGGGGCCCTCATCCCTGCCATCTACATGTTGGTCTTCCTC
  CTGGGCACCACGGGAAACGGTCTGGTGCTCTGGACCGTGTTTCGGAGCAGCCGGGAGAAG
  AGGCGCTCAGCTGATATCTTCATTGCTAGCCTGGCGGTGGCTGACCTGACCTTCGTGGTG
  ACGCTGCCCCTGTGGGCTACCTACACGTACCGGGACTATGACTGGCCCTTTGGGACCTTC
  TTCTGCAAGCTCAGCAGCTACCTCATCTTCGTCAACATGTACGCCAGCGTCTTCTGCCTC
  ACCGGCCTCAGCTTCGACCGCTACCTGGCCATCGTGAGGCCAGTGGCCAATGCTCGGCTG
  AGGCTGCGGGTCAGCGGGGCCGTGGCCACGGCAGTTCTTTGGGTGCTGGCCGCCCTCCTG
  GCCATGCCTGTCATGGTGTTACGCACCACCGGGGACTTGGAGAACACCACTAAGGTGCAG
  TGCTACATGGACTACTCCATGGTGGCCACTGTGAGCTCAGAGTGGGCCTGGGAGGTGGGC
  CTTGGGGTCTCGTCCACCACCGTGGGCTTTGTGGTGCCCTTCACCATCATGCTGACCTGT
  TACTTCTTCATCGCCCAAACCATCGCTGGCCACTTCCGCAAGGAACGCATCGAGGGCCTG
  CGGAAGCGGCGCCGGCTGCTCAGCATCATCGTGGTGCTGGTGGTGACCTTTGCCCTGTGC
  TGGATGCCCTACCACCTGGTGAAGACGCTGTACATGCTGGGCAGCCTGCTGCACTGGCCC
  TGTGACTTTGACCTCTTCCTCATGAACATCTTCCCCTACTGCACCTGCATCAGCTACGTC
  AACAGCTGCCTCAACCCCTTCCTCTATGCCTTTTTCGACCCCCGCTTCCGCCAGGCCTGC
  ACCTCCATGCTCTGCTGTGGCCAGAGCAGGTGCGCAGGCACCTCCCACAGCAGCAGTGGG
  GAGAAGTCAGCCAGCTACTCTTCGGGGCACAGCCAGGGGCCCGGCCCCAACATGGGCAAG
  GGTGGAGAACAGATGCACGAGAAATCCATCCCCTACAGCCAGGAGACCCTTGTGGTTGAC
  TAG
  
  Clone variation with respect to NM_005161.4
  135 c=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005161 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCTTAGCATGGAGGAAGGTGGTGATTT
  TGACAACTACTATGGGGCAGACAACCAGTCTGAGTGTGAGTACACAGACTGGAAATCCTC
  GGGGGCCCTCATCCCTGCCATCTACATGTTGGTCTTCCTCCTGGGCACCACGGGAAACGG
  TCTGGTGCTCTGGACCGTGTTTCGGAGCAGCCGGGAGAAGAGGCGCTCAGCTGATATCTT
  CATTGCTAGCCTGGCGGTGGCTGACCTGACCTTCGTGGTGACGCTGCCCCTGTGGGCTAC
  CTACACGTACCGGGACTATGACTGGCCCTTTGGGACCTTCTTCTGCAAGCTCAGCAGCTA
  CCTCATCTTCGTCAACATGTACGCCAGCGTCTTCTGCCTCACCGGCCTCAGCTTCGACCG
  CTACCTGGCCATCGTGAGGCCAGTGGCCAATGCTCGGCTGAGGCTGCGGGTCAGCGGGGC
  CGTGGCCACGGCAGTTCTTTGGGTGCTGGCCGCCCTCCTGGCCATGCCTGTCATGGTGTT
  ACGCACCACCCGGGACTTTGAGGACACCAACTAAGTGCAATGCTTCATGGACTTACTCAT
  GGTGGCCACTGTGAGCTTAAAGTGGGCCTGGGAGGTGGGCCCTGGGGTCTTGTCCACCAC
  CGTGGGCTTTGTGGTGCCCTTCAACATCATGCTGACCTGGTACCTCTTCATCGCCCAAAC
  CATTCGTTGCCACTCCGGCAGGAACCCAT
  
• Restriction Sites: Please inquire
• ACCN: NM_005161
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005161.2, NP_005152.1
• RefSeq Size: 1833 bp
• RefSeq ORF: 1143 bp
• Locus ID: 187
• UniProt ID: P35414
• Cytogenetics: 11q12.1
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: This gene encodes a member of the G protein-coupled receptor gene family. The encoded protein is related to the angiotensin receptor, but is actually an apelin receptor that inhibits adenylate cyclase activity and plays a counter-regulatory role against the pressure action of angiotensin II by exerting hypertensive effect. It functions in the cardiovascular and central nervous systems, in glucose metabolism, in embryonic and tumor angiogenesis and as a human immunodeficiency virus (HIV-1) coreceptor. Two transcript variants resulting from alternative splicing have been identified. [provided by RefSeq, Jul 2009]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein.