GATA4 (NM_002052) Human Untagged Clone

CAT#: SC124037

GATA4 (untagged)-Human GATA binding protein 4 (GATA4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GATA4
• Synonyms: ASD2; TACHD; TOF; VSD1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC124037 sequence for NM_002052 edited (data generated by NextGen Sequencing)
  ATGTATCAGAGCTTGGCCATGGCCGCCAACCACGGGCCGCCCCCCGGTGCCTACGAGGCGGGCGGCCCCG
  GCGCCTTCATGCACGGCGCGGGCGCCGCGTCCTCGCCAGTCTACGTGCCCACACCGCGGGTGCCCTCCTC
  CGTGCTGGGCCTGTCCTACCTCCAGGGCGGAGGCGCGGGCTCTGCGTCCGGAGGCGCCTCGGGCGGCAGC
  TCCGGTGGGGCCGCGTCTGGTGCGGGGCCCGGGACCCAGCAGGGCAGCCCGGGATGGAGCCAGGCGGGAG
  CCGACGGAGCCGCTTACACCCCGCCGCCGGTGTCGCCGCGCTTCTCCTTCCCGGGGACCACCGGGTCCCT
  GGCGGCCGCCGCCGCCGCTGCCGCGGCCCGGGAAGCTGCGGCCTACAGCAGTGGCGGCGGAGCGGCGGGT
  GCGGGCCTGGCGGGCCGCGAGCAGTACGGGCGCGCCGGCTTCGCGGGCTCCTACTCCAGCCCCTACCCGG
  CTTACATGGCCGACGTGGGCGCGTCCTGGGCCGCAGCCGCCGCCGCCTCCGCCGGCCCCTTCGACAGCCC
  GGTCCTGCACAGCCTGCCCGGCCGGGCCAACCCGGCCGCCCGACACCCCAATCTCGATATGTTTGACGAC
  TTCTCAGAAGGCAGAGAGTGTGTCAACTGTGGGGCTATGTCCACCCCGCTCTGGAGGCGAGATGGGACGG
  GTCACTATCTGTGCAACGCCTGCGGCCTCTACCACAAGATGAACGGCATCAACCGGCCGCTCATCAAGCC
  TCAGCGCCGGCTGTCCGCCTCCCGCCGAGTGGGCCTCTCCTGTGCCAACTGCCAGACCACCACCACCACG
  CTGTGGCGCCGCAATGCGGAGGGCGAGCCTGTGTGCAATGCCTGCGGCCTCTACATGAAGCTCCACGGGG
  TCCCCAGGCCTCTTGCAATGCGGAAAGAGGGGATCCAAACCAGAAAACGGAAGCCCAAGAACCTGAATAA
  ATCTAAGACACCAGCAGCTCCTTCAGGCAGTGAGAGCCTTCCTCCCGCCAGCGGTGCTTCCAGCAACTCC
  AGCAACGCCACCACCAGCAGCAGCGAGGAGATGCGTCCCATCAAGACGGAGCCTGGTCTGTCATCTCACT
  ACGGGCACAGCAGCTCCGTGTCCCAGACGTTCTCAGTCAGTGCGATGTCTGGCCATGGGCCCTCCATCCA
  CCCTGTCCTCTCGGCCCTGAAGCTCTCCCCACAAGGCTATGCGTCTCCCGTCAGCCAGTCTCCACAGACC
  AGCTCCAAGCAGGACTCTTGGAACAGCCTGGTCTTGGCCGACAGTCACGGGGACATAATCACTGCGTAA
  
  Clone variation with respect to NM_002052.3
  353 c=>n;354 g=>n;355 g=>n;356 c=>n;357 c=>n;358 g=>n;359 c=>n;360 c=>n;361 g=>n;1107 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002052 unedited
  GGTCAGATTTGTATACGACTCATATAGGCGGCCGCGAATTCGCACGAGCGCAGGGCCGCG
  AGAGGCTTCGTCGCCGCTGCAGCTCCGGGGGCTCCCAGGGGAGCGTGCGCGGAACCTCCA
  GGCCCAGCAGGACCCCGGCTGCGGCGAGGAGGAAGGAGCCAGCCTAGCAGCTTCTGCGCC
  TGTGGCCGCGGGTGTCCTGGAGGCCTCTCGGTGTGACGAGTGGGGGACCCGAAGGCTCGT
  GCGCCACCTCCAGGCCTGGACGCTGCCCTCCGTCTTCTGCCCCCAATAGGTGCGCCGGAC
  CTTCAGGCCCTGGGGTGAATTCAGCTGCTCCTACATCAGCTTCCGGAACCACCAAAAATT
  CAAATTGGGATTTTCCGGAGTAAACAAGAGCCTAGAGCCCTTTGCTCAATGCTGGATTTA
  ATACGTATATATTTTTAAGCGAGTTGGTTTTTTCCCCTTTGATTTTTGATCTTCGCGACA
  GTTCCTCCCACGCATATTATCGTTGTTGCCGTCGTTTTCTCTCCCCGCGTGGCTCCTTGA
  CCTGCGAGGGAGAGAGAGGACACCGAAGCCGGGAGCTCGCAGGGACCATGTATCAGAGCT
  TGGCCATGGCCGCCAACCACGGGCCGCCCCCCGGTGCCTACGAGGCGGGCGGCCCCGGCG
  CCTTCATGCACGGCGCGGGCGCCGCGTCCTCGCCAGTCTACGTGCCCACACCGCGGGGTG
  CCCTCCTCCGTGCTGGGCCTGTCCTACCTCCAGGGCGGAGGCGCGGGCTCTGCGTCCGGG
  AGCGCCCTCGGCGGCAGCTCCGGGTGGGGCCCGGTCTGTTGCCGGGCCCGGNACCCCACA
  GGGCAGCCCGGGGAGGACCCAGCGCGGGGCCAACGAGCCGCTTACCCCCCGCCGCCGTGT
  CTCCCGCCCTTTTCCTCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002052 unedited
  GCATGGCAACTTCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGG
  ATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTGT
  TTTTTAAAGAGGAATACAGATAAATTTATTAGTTAAATACTGATTTTCCAGCCATTTCAC
  CTTAAGACAATGTTAACAGGTTTGTGGGTTAGGGAGGGTATACGAGGGGGCCTTTGGAAG
  AAAACAATGTAAATGATGATTAAAACAGAATCTTGGTTCAAAGGTATTCTCTGCTACAGC
  CAGTAGGATTTTGGAGTGAGGGGTCTGGGCGTGTGGGGAGGCGTAGTAATGCCACAGTCA
  GCTACAGCTCTGCTGAGAAAGAGGAAGGAGTCTCCATGAGCTCCAGCATCAGGGGCAGAA
  ACAGCAATGTGCAGAGGAGAACGCGGCAGATCCCGGAGCAGCTCAGAGTCGGAGGCTCCC
  TCCAGGCCCCCCTGCCGTGTCTTAGCAGTCGTCTTCTTTCCAGGAATTCTGTTGTGTTGT
  GTTTGTTTGCTTCGAATTCGTGTTGCAGAATCTCTGGCTTTTTGCCTCCTGGACAAAAGA
  CTTCTGCCGGGGCGAAGCTGCAAGTCCTTGAGGACTCTGGCTTGCATCTTGACCAAGGGT
  AGGAGGACCAACAGACCACAGGTGGGCCACCTTGGGACACTCTGCCCCTGACAGGAATTT
  GTCACTTACCTCTCCCAGAAGACCTCAGGCTGGCTGATGCTGCCAATGCTTGACACAAAC
  GTTTGTTTTCTAAACCTTTATTTGACCGTGACAAGGCTCATGACTCAGGGGTCGTTGTAA
  TCCAACCTTGTTCAGGAAAAATTCAACG
  
• Restriction Sites: Please inquire
• ACCN: NM_002052
• Insert Size: 3600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002052.2, NP_002043.2
• RefSeq Size: 3372 bp
• RefSeq ORF: 1329 bp
• Locus ID: 2626
• UniProt ID: P43694
• Cytogenetics: 8p23.1
• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Transcription Factors
• Gene Summary: This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
  Transcript Variant: This variant (2) differs in the 5' UTR and uses an alternate in-frame splice site in the central coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.