CD47 (NM_001777) Human Untagged Clone

CAT#: SC119049

CD47 (untagged)-Human CD47 molecule (CD47), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD47
• Synonyms: IAP; MER6; OA3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001777 edited
  GAATTCGGCACGAGGCGCGTGCGCGCGGCCGTGCAGCCTGGGCAGTGGGTCCTGCCTGTG
  ACGCGCGGCGGCGGTCGGTCCTGCCTGTAACGGCGGCGGCGGCTGCTGCTCCAGACACCT
  GCGGCGGCGGCGGCGACCCCGCGGCGGGCGCGGAGATGTGGCCCCTGGTAGCGGCGCTGT
  TGCTGGGCTCGGCGTGCTGCGGATCAGCTCAGCTACTATTTAATAAAACAAAATCTGTAG
  AATTCACGTTTTGTAATGACACTGTCGTCATTCCATGCTTTGTTACTAATATGGAGGCAC
  AAAACACTACTGAAGTATACGTAAAGTGGAAATTTAAAGGAAGAGATATTTACACCTTTG
  ATGGAGCTCTAAACAAGTCCACTGTCCCCACTGACTTTAGTAGTGCAAAAATTGAAGTCT
  CACAATTACTAAAAGGAGATGCCTCTTTGAAGATGGATAAGAGTGATGCTGTCTCACACA
  CAGGAAACTACACTTGTGAAGTAACAGAATTAACCAGAGAAGGTGAAACGATCATCGAGC
  TAAAATATCGTGTTGTTTCATGGTTTTCTCCAAATGAAAATATTCTTATTGTTATTTTCC
  CAATTTTTGCTATACTCCTGTTCTGGGGACAGTTTGGTATTAAAACACTTAAATATAGAT
  CCGGTGGTATGGATGAGAAAACAATTGCTTTACTTGTTGCTGGACTAGTGATCACTGTCA
  TTGTCATTGTTGGAGCCATTCTTTTCGTCCCAGGTGAATATTCATTAAAGAATGCTACTG
  GCCTTGGTTTAATTGTGACTTCTACAGGGATATTAATATTACTTCACTACTATGTGTTTA
  GTACAGCGATTGGATTAACCTCCTTCGTCATTGCCATATTGGTTATTCAGGTGATAGCCT
  ATATCCTCGCTGTGGTTGGACTGAGTCTCTGTATTGCGGCGTGTATACCAATGCATGGCC
  CTCTTCTGATTTCAGGTTTGAGTATCTTAGCTCTAGCACAATTACTTGGACTAGTTTATA
  TGAAATTTGTGGCTTCCAATCAGAAGACTATACAACCTCCTAGGAAAGCTGTAGAGGAAC
  CCCTTAATGCATTCAAAGAATCAAAAGGAATGATGAATGATGAATAACTGAAGTGAAGTG
  ATGGACTCCGATTTGGAGAGTAGTAAGACGTGAAAGGAATACACTTGTGTTTAAGCACCA
  TGGCCTTGATGATTCACTGTTGGGGAGAAGAAACAAGAAAAGTAACTGGTTGTCACCTAT
  GAGACCCTTACGTGATTGTTAGTTAAGTTTTTATTCAAAGCAGCTGTAATTTAGTTAATA
  AAATAATTATGATCTATGTTGTTTGCCCAATTGAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001777 unedited
  GGCAACACCATTCGGCACGAGGCGCGTGCGCTCGGCCGTGCAGCCTGGGCAGTGGGTCCT
  GCCTGTGACGCGCGGCGGCGGTCGGTCCTGCCTGTAACGGCGGCGGCGGCTGCTGCTCCA
  GACACCTGCGGCGGCGGCGGCGACCCCGCGGCGGGCGCGGAGATGTGGCCCCTGGTAGCT
  GCGTTGTTGCTGGGCTCGGCGTGCTGCGGATCAGCTCAGCTACTATTTAATAAAACAAAA
  TCTGTAGAATTCACGTTTTGTAATGACACTGTCGTCATTCCATGCTTTGTTACTAATATG
  GAGGCACAAAACACTACTGAAGTATACGTAAAGTGGAAATTTAAAGGAAGAGATATTTAC
  ACCTTTGATGGAGCTCTAAACAAGTCCACTGTCCCCACTGACTTTAGTAGTGCAAAAATT
  GAAGTCTCACAATTACTAAAAGGAGATGCCTCTTTGAAGATGGATAAGAGTGATGCTGTC
  TCACACACAGGAAACTACACTTGTGAAGTAACAGAATTAACCAGAGAAGGTGAAACGATC
  ATCGAGCTAAAATATCGTGTTGTTTCATGGTTTTCTCCANATGAAAATATTCTTATTGTT
  ATTTTCCCCAATTTTGCTATACTCCTGTTCTGGGGACAGTTTGGTATTAAAACACTTAAA
  TATAGATCCGGTGGTATGGATGAGAAACAATTGCTTTACTTGTTGCTGGACTAGTGATCA
  CTGTCCATGGCATTGTTGGAAGCCATTCTTTTCGTCCCAGGTGAATATTCTTAAAGAATG
  CTACTGGCCCTGGTTTAATGTGACTTCTACAGGAATATTAATATTACCTTCCTACTTGTG
  TTTAGACAACGATTGAATTACCCCCCTTCGTCATGCCACATTGGGTATTCAGGGGAAAGC
  CT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001777 unedited
  GGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTCAATTGGGCAAACAACATAGA
  TCATAATTATTTTATTAACTAAATTACAGCTGCTTTGAATAAAAACTTAACTAACAATCA
  CGTAAGGGTCTCATAGGTGACAACCAGTTACTTTTCTTGTTTCTTCTCCCCAACAGTGAA
  TCATCAAGGCCATGGTGCTTAAACACAAGTGTATTCCTTTCACGTCTTACTACTCTCCAA
  ATCGGAGTCCATCACTTCACTTCAGTTATTCATCATTCATCATTCCTTTTGATTCTTTGA
  ATGCATTAAGGGGTTCCTCTACAGCTTTCCTAGGAGGTTGTATAGTCTTCTGATTGGAAG
  CCACAAATTTCATATAAACTAGTCCAAGTAATTGTGCTAGAGCTAAGATACTCAAACCTG
  AAATCAGAAGAGGGCCATGCATTGGTATACACGCCGCAATACAGAGACTCAGTCCAACCA
  CAGCGAGGATATAGGCTATCACCTGAATAACCAATATGGCAATGACGAAGGAGGTTAATC
  CAATCGCTGTACTAAACACATAGTAGTGAAGTAATATTAATATCCCTGTAGAAGTCACAA
  TTAAACCAAGGCCAGTAGCATTCTTTAATGAATATTCACCTGGGACGAAAAGAATGGCTC
  CAACAATGACAATGACAGTGATCACTAGTCCAGCAACAAGTAAAGCAATTGTTTTCTCAT
  CCATACCACCGGATCTATATTTAAGTGTTTTAATACCAAACTGTCCCCAAACAGGAGTAT
  AGCANAAATTGGGAAAATACACTAAGATATTTTCATTTGGAGAATACCATGAAACACACG
  ATATTTAGCTCGATGATCGTTTCACCTTCTCTGGNTAAATTCTGTACTTCACAAGTGTAG
  TTNCCCTGGTGTGTGAGACAGCATCACTCTTATNCCATCTCAAGAGGCATCTCCTTCTAG
  TAATNGNGAGACTTCATT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001777
• Insert Size: 1400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001777.3, NP_001768.1
• RefSeq Size: 5346 bp
• RefSeq ORF: 972 bp
• Locus ID: 961
• UniProt ID: Q08722
• Cytogenetics: 3q13.12
• Domains: CD47
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ECM-receptor interaction
• Gene Summary: This gene encodes a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]
  Transcript Variant: This variant (1) encodes the longest isoform (1).