EAAT1 (SLC1A3) (NM_004172) Human Untagged Clone

CAT#: SC117514

SLC1A3 (untagged)-Human solute carrier family 1 (glial high affinity glutamate transporter), member 3 (SLC1A3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EAAT1
• Synonyms: EA6; EAAT1; GLAST; GLAST1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117514 sequence for NM_004172 edited (data generated by NextGen Sequencing)
  ATGACTAAAAGCAATGGAGAAGAGCCCAAGATGGGGGGCAGGATGGAGAGATTCCAGCAG
  GGAGTCCGTAAACGCACACTTTTGGCCAAGAAGAAAGTGCAGAACATTACAAAGGAGGAT
  GTTAAAAGTTACCTGTTTCGGAATGCTTTTGTGCTGCTCACAGTCACCGCTGTCATTGTG
  GGTACAATCCTTGGATTTACCCTCCGACCATACAGAATGAGCTACCGGGAAGTCAAGTAC
  TTCTCCTTTCCTGGGGAACTTCTGATGAGGATGTTACAGATGCTGGTCTTACCACTTATC
  ATCTCCAGTCTTGTCACAGGAATGGCGGCGCTAGATAGTAAGGCATCAGGGAAGATGGGA
  ATGCGAGCTGTAGTCTATTATATGACTACCACCATCATTGCTGTGGTGATTGGCATAATC
  ATTGTCATCATCATCCATCCTGGGAAGGGCACAAAGGAAAACATGCACAGAGAAGGCAAA
  ATTGTACGAGTGACAGCTGCAGATGCCTTCCTGGACTTGATCAGGAACATGTTCCCTCCA
  AATCTGGTAGAAGCCTGCTTTAAACAGTTTAAAACCAACTATGAGAAGAGAAGCTTTAAA
  GTGCCCATCCAGGCCAACGAAACGCTTGTGGGTGCTGTGATAAACAATGTGTCTGAGGCC
  ATGGAGACTCTTACCCGAATCACAGAGGAGCTGGTCCCAGTTCCAGGATCTGTGAATGGA
  GTCAATGCCCTGGGTCTAGTTGTCTTCTCCATGTGCTTCGGTTTTGTGATTGGAAACATG
  AAGGAACAGGGGCAGGCCCTGAGAGAGTTCTTTGATTCTCTTAACGAAGCCATCATGAGA
  CTGGTAGCAGTAATAATGTGGTATGCCCCCGTGGGTATTCTCTTCCTGATTGCTGGGAAG
  ATTGTGGAGATGGAAGACATGGGTGTGATTGGGGGGCAGCTTGCCATGTACACCGTGACT
  GTCATTGTTGGCTTACTCATTCACGCAGTCATCGTCTTGTCACTCCTCTACTTCTTGGTA
  ACACGGAAAAACCCTTGGGTTTTTATTGGAGGGTTGCTGCAAGCACTCATCACCGCTCTG
  GGGACCTCTTCAAGTTCTGCCACCCTACCCATCACCTTCAAGTGCCTGGAAGAGAACAAT
  GGCGTGGACAAGCGCGTCACCAGATTCGTGCTCCCCGTAGGAGCCACCATTAACATGGAT
  GGGACTGCCCTCTATGAGGCTTTGGCTGCCATTTTCATTGCTCAAGTTAACAACTTTGAA
  CTGAACTTCGGACAAATTATTACAATCAGCATCACAGCCACAGCTGCCAGTATTGGGGCA
  GCTGGAATTCCTCAGGCGGGCCTGGTCACTATGGTCATTGTGCTGACATCTGTCGGCCTG
  CCCACTGACGACATCACGCTCATCATCGCGGTGGACTGGTTCCTGGATCGCCTCCGGACC
  ACCACCAACGTACTGGGAGACTCCCTGGGAGCTGGGATTGTGGAGCACTTGTCACGACAT
  GAACTGAAGAACAGAGATGTTGAAATGGGTAACTCAGTGATTGAAGAGAATGAAATGAAG
  AAACCATATCAACTGATTGCACAGGACAATGAAACTGAGAAACCCATCGACAGTGAAACC
  AAGATGTAG
  
  Clone variation with respect to NM_004172.4
  1000 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004172 unedited
  GGGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGTCAGGGC
  TAGCCTGCCTGCTTACGCGCGCTGCGGATTGTTGCTCCGTTGTACCTGCTGGGGAATTCA
  CCTCGTTACTGCTTGATATCTTCCACCCCTTACAAAATCAGAAAAGTTGTGTTTTCTAAT
  ACCAAAGAGGAGGTTTGGCTTTCTGTGGGTGATTCCCAGACACTGAAGTGCAAAGAAGAG
  ACCCTCCTAGAAAAGTAAAATATGACTAAAAGCAATGGAGAAGAGCCCAAGATGGGGGGC
  AGGATGGAGAGATTCCAGCAGGGAGTCCGTAAACGCACACTTTTGGCCAAGAAGAAAGTG
  CAGAACATTACAAAGGAGGATGTTAAAAGTTACCTGTTTCGGAATGCTTTTGTGCTGCTC
  ACAGTCACCGCTGTCATTGTGGGTACAATCCTTGGATTTACCCTCCGACCATACAGAATG
  AGCTACCGGGAAGTCAAGTACTTCTCCTTTCCTGGGGAACTTCTGATGAGGATGTTACAG
  ATGCTGGTCTTACCACTTATCATCTCCAGTCTTGTCACAGGAATGGCGGCGCTAGATAGT
  AAGGCATCAGGGAAGATGGGAATGCGAGCTGTAGTCTATTATATGACTACCACCATCATT
  GCTGTGGTGATTGGCATAATCATTGTCATCATCATCCATCCTGNGAAGGGCACAAAGGAA
  AACATGCACAGAGAAGGCAAAATTGTACGAGTGACAGCTGCAGATGCCTTCCTGGACTTG
  ATCAGGAACATGTTCCCTTNCANATCTGTAGAAGCCTGCTTTANACAGTTTAAAACCAAC
  TATGAGAAGAGAAGCTNNTAAGTGCCCATCCAGACCAACGAAACGCTTGTGGGTGCTGTG
  ATAAACAATGTGTCTGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004172 unedited
  GCAACTTTCCAGGGCCAGGNAAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATC
  TGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTT
  TTTTAATGTAAAACTATTTTTTTCTTTATTGAAAATACATCTACAAAGTAATGCTTCCCA
  GTCCACACTGAGTGCATGTGTATGCAAAGCCTGTGATGGCTTGGCGAGGGGATGGGCTCC
  CCACAGGACCTTTCCATAAGAAATGGTTAGTATATTAAAAAACACATATTGTGTAACAGA
  ATTAAGTTGATATGATTAAGTCTTTGCTCTACTAGTGTCTTAAGAGGACAGCCACGTACT
  TTAACATTTGTAGTGTATTTCCGAAATAGAGCCTCGACATTAAATGGTTACTGCACCACA
  GCCTAATGTTAACTTTTATGCACAGAATGCTTGGGGTCTATATAAAAATATCTCTAATTG
  TATATCAGAGGATCTATGTCTAGATTTTTCAGTTTGTTAGCATAATTAAGTCCATTCTTC
  ACCATTTTCAGCCAGAAACTCAAACACAGCTGCTACTGCCTGACAGCTCCCACTCAGCGT
  CTTTGACTGGATATTCCTTANGGGGTAAAAATTAATCTCTAAACCTGGNGAGCTGCCTTG
  CCTGAAACTAATGCCCNCCTGCTGTTTTTTTCTCATAAACCTCATTGACAGNNGNCTTCA
  TACTGGGATTCATCTTCTACCATTTTCTCTTNATGGNNACACAAACANACANAAAGCAAC
  CCCACTTCAGCNTGAAAGTTTTTGAATGATTTGGAATAAGTGGGGATTCCAAACAAATAA
  AATATTTTGGTTTCCCTCATTCTAAGGGACCTTTGGATTAAAGAAAAACACTTTCNATAA
  NGAATACCCACTCACTCCTGGGACTTCTTTAAAGAACTCATCTTTTGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004172
• Insert Size: 1629 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004172.3, NP_004163.2
• RefSeq Size: 3983 bp
• RefSeq ORF: 1629 bp
• Locus ID: 6507
• UniProt ID: P43003
• Cytogenetics: 5p13.2
• Domains: SDF
• Protein Families: Transmembrane
• Gene Summary: This gene encodes a member of a member of a high affinity glutamate transporter family. This gene functions in the termination of excitatory neurotransmission in central nervous system. Mutations are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Feb 2014]
  Transcript Variant: This variant (GLAST) represents the longest transcript and encodes the longest protein (isoform 1).