SAMHD1 (NM_015474) Human Untagged Clone

CAT#: SC114650

SAMHD1 (untagged)-Human SAM domain and HD domain 1 (SAMHD1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SAMHD1
• Synonyms: CHBL2; DCIP; HDDC1; hSAMHD1; MOP-5; SBBI88
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114650 sequence for NM_015474 edited (data generated by NextGen Sequencing)
  ATGCAGCGAGCCGATTCCGAGCAGCCCTCCAAGCGTCCCCGTTGCGATGACAGCCCGAGA
  ACCCCCTCAAACACCCCTTCCGCAGAGGCAGACTGGTCCCCGGGCCTGGAACTCCATCCC
  GACTACAAGACATGGGGTCCGGAGCAGGTGTGCTCCTTCCTCAGGCGCGGTGGCTTTGAA
  GAGCCGGTGCTGCTGAAGAACATCCGAGAAAATGAAATCACAGGCGCATTACTGCCTTGT
  CTTGATGAGTCTCGTTTTGAAAATCTTGGAGTAAGTTCCTTGGGGGAGAGGAAGAAGCTG
  CTTAGTTATATCCAGCGATTGGTTCAAATCCACGTTGATACAATGAAGGTAATTAATGAT
  CCTATCCATGGCCACATTGAGCTCCACCCTCTCCTCGTCCGAATCATTGATACACCTCAA
  TTTCAACGTCTTCGATACATCAAACAGCTGGGAGGTGGTTACTATGTTTTTCCAGGAGCT
  TCACACAATCGATTTGAGCATAGTCTAGGGGTGGGGTATCTAGCAGGATGTCTAGTTCAC
  GCACTGGGTGAAAAACAACCAGAGCTGCAGATAAGTGAACGAGATGTTCTCTGTGTTCAG
  ATTGCTGGACTTTGTCATGATCTCGGTCATGGGCCATTTTCTCACATGTTTGATGGACGA
  TTTATTCCACTTGCTCGCCCGGAGGTGAAATGGACGCATGAACAAGGCTCAGTTATGATG
  TTTGAGCACCTTATTAATTCTAATGGAATTAAGCCTGTCATGGAACAATATGGTCTCATC
  CCTGAAGAAGATATTTGCTTTATAAAGGAACAAATTGTAGGACCACTTGAATCACCTGTC
  GAAGATTCATTGTGGCCATATAAAGGGCGTCCTGAAAACAAAAGCTTCCTTTATGAGATA
  GTATCTAATAAAAGAAATGGCATTGATGTGGACAAATGGGATTATTTTGCCAGGGACTGC
  CATCATCTTGGAATCCAAAATAATTTTGATTACAAGCGCTTTATTAAGTTTGCCCGTGTC
  TGTGAAGTAGACAATGAGTTGCGTATTTGTGCTAGAGATAAGGAAGTTGGAAATCTGTAT
  GACATGTTCCACACTCGCAACTCTTTACACCGTAGAGCTTATCAACACAAAGTTGGCAAC
  ATTATTGATACAATGATTACAGATGCTTTCCTCAAAGCAGATGACTACATAGAGATTACA
  GGTGCTGGAGGAAAAAAGTATCGCATTTCTACAGCAATTGACGACATGGAAGCCTATACT
  AAGCTGACAGATAACATTTTTCTGGAGATTTTATACTCTACTGATCCCAAATTGAAAGAC
  GCACGAGAGATTTTAAAACAAATTGAATACCGTAATCTATTCAAGTATGTGGGTGAGACG
  CAGCCAACAGGACAAATAAAGATTAAAAGGGAGGACTATGAATCTCTTCCAAAAGAGGTT
  GCCAGTGCTAAACCCAAAGTATTGCTAGACGTGAAACTGAAGGCTGAAGATTTTATAGTG
  GATGTTATCAACATGGATTATGGAATGCAAGAAAAGAATCCAATTGATCATGTTAGCTTC
  TATTGTAAGACTGCCCCCAACAGAGCAATCAGGATTACTAAAAACCAGGTTTCACAACTT
  CTGCCAGAGAAATTTGCAGAGCAGCTGATTCGAGTATATTGTAAGAAGGTGGACAGAAAG
  AGTTTGTATGCCGCAAGACAATATTTTGTTCAGTGGTGTGCAGACAGAAATTTCACCAAG
  CCGCAGGATGGCGATGTTATAGCCCCACTCATAACACCTCAAAAAAAGGAATGGAACGAC
  AGTACTTCAGTCCAAAATCCAACTCGCCTCCGAGAAGCATCCAAAAGCAGAGTCCAGCTT
  TTTAAAGATGACCCAATGTGA
  
  Clone variation with respect to NM_015474.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015474 unedited
  GGGTGGTCAGCATTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACCAGGGGCCC
  TGCTGTCAGTGCGCCTGCGCGCGGGTCCGGCGCCGAGGTTCTTGACTGCTGTGCCGGACG
  CCAGGTGTAGCCATGCAGCGAGCCGATTCCGAGCAGCCCTCCAAGCGTCCCCGTTGCGAT
  GACAGCCCGAGAACCCCCTCAAACACCCCTTCCGCAGAGGCAGACTGGTCCCCGGGCCTG
  GAACTCCATCCCGACTACAAGACATGGGGTCCGGAGCAGGTGTGCTCCTTCCTCAGGCGC
  GGTGGCTTTGAAGAGCCGGTGCTGCTGAAGAACATCCGAGAAAATGAAATCACAGGCGCA
  TTACTGCCTTGTCTTGATGAGTCTCGTTTTGAAAATCTTGGAGTAAGTTCCTTGGGGGAG
  AGGAAGAAGCTGCTTAGTTATATCCAGCGATTGGTTCAAATCCACGTTGATACAATGAAG
  GTAATTAATGATCCTATCCATGGCCACATTGAGCTCCACCCTCTCCTCGTCCGAATCATT
  GATACACCTCAATTTCAACGTCTTCGATACATCAAACAGCTGGGAGGTGGTTACTATGTT
  TTTCCAGGAGCTTCACACAATCGATTTGAGCATAGTCTAGGGGTGGGGTATCTAGCAGGA
  TGTCTAGTTCACGCACTGGGTGAAAAACAACCAGAGCTGCAGATAAGTGAACGAGATGTT
  CTCTGTGTTCAGATTGCTGGACTTTGTCATGATCTCGGTCATGGGCCATTTTCTCACATG
  TTTGATGGACGATTTATTCCACTTGCTCGCCCGGNAGGTGAAATGGACGCATGAACAAGG
  CTCAGTTATGATGTTTGAGCACCTTATTAATTCTATGGGATNAGCCTGTCATGGACN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015474 unedited
  NANGATCGAGTTTTTTTTTTTTTTTTTTGAAGGAGAAAGCTTTAATAATATTAAAAATAG
  CAAGGTAATGCTTTTCATAGTATAGTAAGATTTGCCTAATACCATCTTATTTCTTTGATT
  AAAAGTTACTTAGCTTCAGCATGCGTGTACATTTAAAATACAAAATTAAAGCATGAGTTG
  TCATTAATTTGCAGAATTCTATGATTGAAGCCTCTAAATGAATTGTGCAGGAGAGGGAGT
  TTGTAAACAACTGACTACAGACATTCACATTGGGTCATCTTTAAAAAGCTGGACTCTGCT
  TTTGGATGCTTCTCGGAGGCGAGTTGGATTTTGGACTGAAGTACTGTCGTTCCATTCCTT
  TTTTTGAGGTGTTATGAGTGGGGCTATAACATCGCCATCCTGCGGCTTGGTGAAATTTCT
  GTCTGCCCACCACTGAACAAAATATTGTCTTGCGGCATACAAACTCTTTCTGTCCACCTT
  CTTACAATATACTCGAATCAACTGCTCTGCAAATTTTTCTGGCAGAAATTGTGAAACCCG
  GGTTTTAATAAATCCTGATTGCTCTGTTGGGGGCAATCTTACCATAGAAGCTAACATGGT
  CAAATGGATTCTTTTCTTGGTTTCCTAAATCCTGGTGGGAAACTCCACTATAAAATCTTC
  AGCCTTCAGGTTTAACTTTTAGCAATACTTTGGGTTTAACCCTGGGCACCCTTTTTTGGG
  AAAAAATAATAAGCGCCCCCTTTTAAAACCTTATTTGGCCGGGGGGGGGGGGGGTAAACC
  CAATACTTGGATAAATTACCGGGTTCCAAATTTGGTTTAAAAAACCTCCCGGTGCGGCTT
  TCTAATATGGGGTCCACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015474
• Insert Size: 2300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015474.2, NP_056289.2
• RefSeq Size: 2701 bp
• RefSeq ORF: 1881 bp
• Locus ID: 25939
• UniProt ID: Q9Y3Z3
• Cytogenetics: 20q11.23
• Domains: SAM, HD
• Gene Summary: This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome. [provided by RefSeq, Mar 2010]