TLR7 (NM_016562) Human Untagged Clone

CAT#: SC114214

TLR7 (untagged)-Human toll-like receptor 7 (TLR7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TLR7
• Synonyms: IMD74; TLR7-like
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_016562 edited
  GAATTCGGCACGAGGCCAGATATAGGATCACTCCATGCCATCAAGAAAGTTGATGCTATT
  GGGCCCATCTCAAGCTGATCTTGGCACCTCTCATGCTCTGCTCTCTTCAACCAGACCTCT
  ACATTCCATTTTGGAAGAAGACTAAAAATGGTGTTTCCAATGTGGACACTGAAGAGACAA
  ATTCTTATCCTTTTTAACATAATCCTAATTTCCAAACTCCTTGGGGCTAGATGGTTTCCT
  AAAACTCTGCCCTGTGATGTCACTCTGGATGTTCCAAAGAACCATGTGATCGTGGACTGC
  ACAGACAAGCATTTGACAGAAATTCCTGGAGGTATTCCCACGAACACCACGAACCTCACC
  CTCACCATTAACCACATACCAGACATCTCCCCAGCGTCCTTTCACAGACTGGACCATCTG
  GTAGAGATCGATTTCAGATGCAACTGTGTACCTATTCCACTGGGGTCAAAAAACAACATG
  TGCATCAAGAGGCTGCAGATTAAACCCAGAAGCTTTAGTGGACTCACTTATTTAAAATCC
  CTTTACCTGGATGGAAACCAGCTACTAGAGATACCGCAGGGCCTCCCGCCTAGCTTACAG
  CTTCTCAGCCTTGAGGCCAACAACATCTTTTCCATCAGAAAAGAGAATCTAACAGAACTG
  GCCAACATAGAAATACTCTACCTGGGCCAAAACTGTTATTATCGAAATCCTTGTTATGTT
  TCATATTCAATAGAGAAAGATGCCTTCCTAAACTTGACAAAGTTAAAAGTGCTCTCCCTG
  AAAGATAACAATGTCACAGCCGTCCCTACTGTTTTGCCATCTACTTTAACAGAACTATAT
  CTCTACAACAACATGATTGCAAAAATCCAAGAAGATGATTTTAATAACCTCAACCAATTA
  CAAATTCTTGACCTAAGTGGAAATTGCCCTCGTTGTTATAATGCCCCATTTCCTTGTGCG
  CCGTGTAAAAATAATTCTCCCCTACAGATCCCTGTAAATGCTTTTGATGCGCTGACAGAA
  TTAAAAGTTTTACGTCTACACAGTAACTCTCTTCAGCATGTGCCCCCAAGATGGTTTAAG
  AACATCAACAAACTCCAGGAACTGGATCTGTCCCAAAACTTCTTGGCCAAAGAAATTGGG
  GATGCTAAATTTCTGCATTTTCTCCCCAGCCTCATCCAATTGGATCTGTCTTTCAATTTT
  GAACTTCAGGTCTATCGTGCATCTATGAATCTATCACAAGCATTTTCTTCACTGAAAAGC
  CTGAAAATTCTGCGGATCAGAGGATATGTCTTTAAAGAGTTGAAAAGCTTTAACCTCTCG
  CCATTACATAATCTTCAAAATCTTGAAGTTCTTGATCTTGGCACTAACTTTATAAAAATT
  GCTAACCTCAGCATGTTTAAACAATTTAAAAGACTGAAAGTCATAGATCTTTCAGTGAAT
  AAAATATCACCTTCAGGAGATTCAAGTGAAGTTGGCTTCTGCTCAAATGCCAGAACTTCT
  GTAGAAAGTTATGAACCCCAGGTCCTGGAACAATTACATTATTTCAGATATGATAAGTAT
  GCAAGGAGTTGCAGATTCAAAAACAAAGAGGCTTCTTTCATGTCTGTTAATGAAAGCTGC
  TACAAGTATGGGCAGACCTTGGATCTAAGTAAAAATAGTATATTTTTTGTCAAGTCCTCT
  GATTTTCAGCATCTTTCTTTCCTCAAATGCCTGAATCTGTCAGGAAATCTCATTAGCCAA
  ACTCTTAATGGCAGTGAATTCCAACCTTTAGCAGAGCTGAGATATTTGGACTTCTCCAAC
  AACCGGCTTGATTTACTCCATTCAACAGCATTTGAAGAGCTTCACAAACTGGAAGTTCTG
  GATATAAGCAGTAATAGCCATTATTTTCAATCAGAAGGAATTACTCATATGCTAAACTTT
  ACCAAGAACCTAAAGGTTCTGCAGAAACTGATGATGAACGACAATGACATCTCTTCCTCC
  ACCAGCAGGACCATGGAGAGTGAGTCTCTTAGAACTCTGGAATTCAGAGGAAATCACTTA
  GATGTTTTATGGAGAGAAGGTGATAACAGATACTTACAATTATTCAAGAATCTGCTAAAA
  TTAGAGGAATTAGACATCTCTAAAAATTCCCTAAGTTTCTTGCCTTCTGGAGTTTTTGAT
  GGTATGCCTCCAAATCTAAAGAATCTCTCTTTGGCCAAAAATGGGCTCAAATCTTTCAGT
  TGGAAGAAACTCCAGTGTCTAAAGAACCTGGAAACTTTGGACCTCAGCCACAACCAACTG
  ACCACTGTCCCTGAGAGATTATCCAACTGTTCCAGAAGCCTCAAGAATCTGATTCTTAAG
  AATAATCAAATCAGGAGTCTGACGAAGTATTTTCTACAAGATGCCTTCCAGTTGCGATAT
  CTGGATCTCAGCTCAAATAAAATCCAGATGATCCAAAAGACCAGCTTCCCAGAAAATGTC
  CTCAACAATCTGAAGATGTTGCTTTTGCATCATAATCGGTTTCTGTGCACCTGTGATGCT
  GTGTGGTTTGTCTGGTGGGTTAACCATACGGAGGTGACTATTCCTTACCTGGCCACAGAT
  GTGACTTGTGTGGGGCCAGGAGCACACAAGGGCCAAAGTGTGATCTCCCTGGATCTGTAC
  ACCTGTGAGTTAGATCTGACTAACCTGATTCTGTTCTCACTTTCCATATCTGTATCTCTC
  TTTCTCATGGTGATGATGACAGCAAGTCACCTCTATTTCTGGGATGTGTGGTATATTTAC
  CATTTCTGTAAGGCCAAGATAAAGGGGTATCAGCGTCTAATATCACCAGACTGTTGCTAT
  GATGCTTTTATTGTGTATGACACTAAAGACCCAGCTGTGACCGAGTGGGTTTTGGCTGAG
  CTGGTGGCCAAACTGGAAGACCCAAGAGAGAAACATTTTAATTTATGTCTCGAGGAAAGG
  GACTGGTTACCAGGGCAGCCAGTTCTGGAAAACCTTTCCCAGAGCATACAGCTTAGCAAA
  AAGACAGTGTTTGTGATGACAGACAAGTATGCAAAGACTGAAAATTTTAAGATAGCATTT
  TACTTGTCCCATCAGAGGCTCATGGATGAAAAAGTTGATGTGATTATCTTGATATTTCTT
  GAGAAGCCCTTTCAGAAGTCCAAGTTCCTCCAGCTCCGGAAAAGGCTCTGTGGGAGTTCT
  GTCCTTGAGTGGCCAACAAACCCGCAAGCTCACCCATACTTCTGGCAGTGTCTAAAGAAC
  GCCCTGGCCACAGACAATCATGTGGCCTATAGTCAGGTGTTCAAGGAAACGGTCTAGCCC
  TTCTTTGCAAAACACAACTGCCTAGTTTACCAAGGAGAGGCCTGGCTGTTTAAATTGTTT
  TCATATATATCACACCAAAAGCGTGTTTTGAAATTCTTCAAGAAATGAGATTGCCCATAT
  TTCAGGGGAGCCACCAACGTCTGTCACAGGAGTTGGAAAGATGGGGTTTATATAATGCAT
  CAAGTCTTCTTTCTTATCTCTCTGTGTCTCTATTTGCACTTGAGTCTCTCACCTCAGCTC
  CTGTAAAAGAGTGGCAAGTAAAAAACATGGGGCTCTGATTCTCCTGTAATTGTGATAATT
  AAATATACACACAATCATGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016562 unedited
  GTAAGATAGAATTGTATACCACTCATATAGGCGGCCGACGAATTCGCACGAGGCCAGNAA
  TAGGATCACTCCATGCCATCAAGAAAGTTGATGCTATTGGGCCCATCTCAAGCTGATCTT
  GGCACCTCTCATGCTCTGCTCTCTTCAACCAGACCTCTACATTCCATTTTGGAAGAAGAC
  TAAAAATGGTGTTTCCAATGTGGACACTGAAGAGACAAATTCTTATCCTTTTTAACATAA
  TCCTAATTTCCAAACTCCTTGGGGCTAGATGGTTTCCTAAAACTCTGCCCTGTGATGTCA
  CTCTGGATGTTCCAAAGAACCATGTGATCGTGGACTGCACAGACAAGCATTTGACAGAAA
  TTCCTGGAGGTATTCCCACGAACACCACGAACCTCACCCTCACCATTAACCACATACCAG
  ACATCTCCCCAGCGTCCTTTCACAGACTGGACCATCTGGTAGAGATCGATTTCAGATGCA
  ACTGTGTACCTATTCCACTGGGGTCAAAAAACAACATGTGCATCAAGAGGCTGCAGATTA
  AACCCAGAAGCTTTAGTGGACTCACTTATTTAAAATCCCTTTACCTGGATGGAAACCAGC
  TACTAGAGATACCGCAGGGCCTCCCGCCTAGCTTACAGCTTCTCAGCCTTGAGGCCAACA
  ACATCTTTTCCATCAGAAAAGAGAATCTAACAGAACTGGCCAACATAGAAATACTCTACC
  TGGGCCAAAACTGTTATTATCGAAATCCCTTGTTATGTTCATATTCAATAGAGAAAGATG
  CCTTTCCTAACTTGACAAGTTAAAAGTGCTCTCCCTGAAAGATAACATGTCACAGCCGTC
  CCTACTGNTTTGCCATCTACTTAAACAGAACTATATCTCTACACAACATGATTTGCAAAT
  CCAGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016562 unedited
  GGTGTATTTTATTTTACATTACAGGNANATCAGAGCCCCTGTTTTTTACTTGCCACTCTT
  TTACAGGAGCTGAGGTGAGAGACTCAAGTGCAAATAGAGACACAGAGAGATAAGAAAGAA
  GACTTGATGCATTATATAAACCCCATCTTTCCAACTCCTGTGACAGACGTTGGTGGCTCC
  CCTGAAATATGGGCAATCTCATTTCTTGAAGAATTTCAAAACACGCTTTTGGTGTGATAT
  ATATGAAAACAATTTAAACAGCCAGGCCTCTCCTTGGTAAACTAGGCAGTTGTGTTTTGC
  AAAGAAGGGCTAGACCGTTTCCTTGAACACCTGACTATAGGCCACATGATTGTCTGTGGC
  CAGGGCGTTCTTTAGACACTGCCAGAAGTATGGGTGAGCTCGCGGGTTTGTTGGCCACTC
  AAGGACAGAACTCTCACATAGCCTTTTCCGGAGCTGGAGGAACTTGGACTTCTGAAAGGG
  CTTCTCAAGTAATATCNNCCCCATCCATTAACTTTTTCATCCCTGACCCTCTGATGGCAC
  ATGTTAAACGCTTTCTTAAAATATTTACTCTTCGCATACTCGTCTGTCATTACAACCCTG
  TCTTCATCGTNAACGCATGCTTTCGGCATGGCCTCCCACACCTGGCTTCCCTAGTTACCA
  GCTCCCTTCTTTCCAGACTCAATTCATTGCTNNCATACCTGCGCCCTCCCCTTCGCCCCC
  CTTGCATACATATCCCACTCCCCCTCCCCTCCTCCTTGTAGCCTCCCCGTCACCACATTC
  CCCTTTNCTCCAGCCCTGCGATTTACATTCGCGCAACCTATTTTATTTTGCCCTTTGTAC
  TCTTTACAGTAGCCCCATCTCTNAAACGCAGCCCTTTAGTTAACACCTGACNNTTCCNCC
  GTCCTCCCTCTCTTCCCCTCGCTTGTCTCTTCGCTNCCTCCCTCCCCGCCAATGTTCTTC
  TTTTCGCTCGCCTTTCCCCCTTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016562
• Insert Size: 3630 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016562.3, NP_057646.1
• RefSeq Size: 4992 bp
• RefSeq ORF: 3150 bp
• Locus ID: 51284
• UniProt ID: Q9NYK1
• Cytogenetics: Xp22.2
• Domains: TIR, LRRCT, LRR, LRR_TYP, LRR_SD22, LRR_BAC
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Toll-like receptor signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The human TLR family comprises 11 members. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. For the recognition of structural components in foreign microorganisms, the various TLRs exhibit different patterns of expression as well; in this way for example, TLR-3, -7, and -8 are essential in the recognition of single-stranded RNA viruses. TLR7 senses single-stranded RNA oligonucleotides containing guanosine- and uridine-rich sequences from RNA viruses, a recognition occuring in the endosomes of plasmacytoid dendritic cells and B cells. This gene is predominantly expressed in lung, placenta, and spleen, and is phylogenetically related and lies in close proximity to another family member, TLR8, on chromosome X. [provided by RefSeq, Aug 2020]