TLR7 Rabbit Polyclonal Antibody

CAT#: TA382572

TLR7 Rabbit polyclonal Antibody

Product Images

Western blot analysis of extracts of Mouse liver, using TLR7 antibody (TA382572) at 1:1000 dilution.|Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25ug per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 90s.

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  Immunofluorescence analysis of mouse spleen cells using TLR7 Rabbit pAb (TA382572) at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of rat spleen cells using TLR7 Rabbit pAb (TA382572) at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.

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  Immunofluorescence analysis of THP-1 cells using TLR7 Rabbit pAb (TA382572) at dilution of 1:25 (40x lens). Blue: DAPI for nuclear staining.

Specifications

Product Data

• Applications: ICC/IF, WB
• Recommended Dilution: WB,1:500 - 1:2000
  IF,1:50 - 1:200
• Reactivities: Human, Mouse, Rat
• Modifications: Unmodified
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-230 of human TLR7 (NP_057646.1).
• Formulation: Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
• Concentration: lot specific
• Purification: Affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20℃. Avoid freeze / thaw cycles.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 120kDa
• Gene Name: toll like receptor 7
• Database Link:
  Entrez Gene 51284 Human
  UniProt ID Q9NYK1
• Background: The protein encoded by this gene is a member of the Toll-like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. They recognize pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. This gene is predominantly expressed in lung, placenta, and spleen, and lies in close proximity to another family member, TLR8, on chromosome X.
• Synonyms: TLR7