PPP2R1A (NM_014225) Human Untagged Clone

CAT#: SC110980

PPP2R1A (untagged)-Human protein phosphatase 2, regulatory subunit A, alpha (PPP2R1A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2R1A
• Synonyms: MRD36; PP2A-Aalpha; PP2AA; PP2AAALPHA; PR65A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110980 sequence for NM_014225 edited (data generated by NextGen Sequencing)
  ATGGCGGCGGCCGACGGCGACGACTCGCTGTACCCCATCGCGGTGCTCATAGACGAACTC
  CGCAATGAGGACGTTCAGCTTCGCCTCAACAGCATCAAGAAGCTGTCCACCATCGCCTTG
  GCCCTTGGGGTTGAAAGGACCCGAAGTGAGCTTCTGCCTTTCCTTACAGATACCATCTAT
  GATGAAGATGAGGTCCTCCTGGCCCTGGCAGAACAGCTGGGAACCTTCACTACCCTGGTG
  GGAGGCCCAGAGTACGTGCACTGCCTGCTGCCACCGCTGGAGTCGCTGGCCACAGTGGAG
  GAGACAGTGGTGCGGGACAAGGCAGTGGAGTCCTTACGGGCCATCTCACACGAGCACTCG
  CCCTCTGACCTGGAGGCGCACTTTGTGCCGCTAGTGAAGCGGCTGGCGGGCGGCGACTGG
  TTCACCTCCCGCACCTCGGCCTGCGGCCTCTTCTCCGTCTGCTACCCCCGAGTGTCCAGT
  GCTGTGAAGGCGGAACTTCGACAGTACTTCCGGAACCTGTGCTCAGATGACACCCCCATG
  GTGCGGCGGGCCGCAGCCTCCAAGCTGGGGGAGTTTGCCAAGGTGCTGGAGCTGGACAAC
  GTCAAGAGTGAGATCATCCCCATGTTCTCCAACCTGGCCTCTGACGAGCAGGACTCGGTG
  CGGCTGCTGGCGGTGGAGGCGTGCGTGAACATCGCCCAGCTTCTGCCCCAGGAGGATCTG
  GAGGCCCTGGTGATGCCCACTCTGCGCCAGGCCGCTGAAGACAAGTCCTGGCGCGTCCGC
  TACATGGTGGCTGACAAGTTCACAGAGCTCCAGAAAGCAGTGGGGCCTGAGATCACCAAG
  ACAGACCTGGTCCCTGCCTTCCAGAACCTGATGAAAGACTGTGAGGCCGAGGTGAGGGCC
  GCAGCCTCCCACAAGGTCAAAGAGTTCTGTGAAAACCTCTCAGCTGACTGTCGGGAGAAT
  GTGATCATGTCCCAGATCTTGCCCTGCATCAAGGAGCTGGTGTCCGATGCCAACCAACAT
  GTCAAGTCTGCCCTGGCCTCAGTCATCATGGGTCTCTCTCCCATCTTGGGCAAAGACAAC
  ACCATCGAGCACCTCTTGCCCCTCTTCCTGGCTCAGCTGAAGGATGAGTGCCCTGAGGTA
  CGGCTGAACATCATCTCTAACCTGGACTGTGTGAACGAGGTGATTGGCATCCGGCAGCTG
  TCCCAGTCCCTGCTCCCTGCCATTGTGGAGCTGGCTGAGGACGCCAAGTGGCGGGTGCGG
  CTGGCCATCATTGAGTACATGCCCCTCCTGGCTGGACAGCTGGGAGTGGAGTTCTTTGAT
  GAGAAACTTAACTCCTTGTGCATGGCCTGGCTTGTGGATCATGTATATGCCATCCGCGAG
  GCAGCCACCAGCAACCTGAAGAAGCTAGTGGAAAAGTTTGGGAAGGAGTGGGCCCATGCC
  ACAATCATCCCCAAGGTCTTGGCCATGTCCGGAGACCCCAACTACCTGCACCGCATGACT
  ACGCTCTTCTGCATCAATGTGCTGTCTGAGGTCTGTGGGCAGGACATCACCACCAAGCAC
  ATGCTACCCACGGTTCTGCGCATGGCTGGGGACCCGGTTGCCAATGTCCGCTTCAATGTG
  GCCAAGTCTCTGCAGAAGATAGGGCCCATCCTGGACAACAGCACCTTGCAGAGTGAAGTC
  AAGCCCATCCTAGAGAAGCTGACCCAGGACCAGGATGTGGACGTCAAATACTTTGCCCAG
  GAGGCTCTGACTGTTCTGTCTCTCGCCTGA
  
  Clone variation with respect to NM_014225.5
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014225 unedited
  NGGTTCAGGATTTTGTNATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGTGGC
  CGCATCTGACAGGAAGGGACGGAGCCAAGATGGCGGCGGCCGACGGCGACGACTCGCTGT
  ACCCCATCGCGGTGCTCATAGACGAACTCCGCAATGAGGACGTTCAGCTTCGCCTCAACA
  GCATCAAGAAGCTGTCCACCATCGCCTTGGCCCTTGGGGTTGAAAGGACCCGAAGTGAGC
  TTCTGCCTTTCCTTACAGATACCATCTATGATGAAGATGAGGTCCTCCTGGCCCTGGCAG
  AACAGCTGGGAACCTTCACTACCCTGGTGGGAGGCCCAGAGTACGTGCACTGCCTGCTGC
  CACCGCTGGAGTCGCTGGCCACAGTGGAGGAGACAGTGGTGCGGGACAAGGCAGTGGAGT
  CCTTACGGGCCATCTCACACGAGCACTCGCCCTCTGACCTGGAGGCGCACTTTGTGCCGC
  TAGTGAAGCGGCTGGCGGGCGGCGACTGGTTCACCTCCCGCACCTCGGCCTGCGGCCTCT
  TCTCCGTCTGCTACCCCCGAGTGTCCAGTGCTGTGAAGGCGGAACTTCGACAGTACTTCC
  GGAACCTGTGCTCAGATGACACCCCCATGGTGCGGCGGGCCGCAGCCTCCAAGCTGGGGG
  AGTTTGCCAAGGTGCTGGAGCTGGACAACGTCAAGAGTGAGATCATCCCCATGTTCTCCA
  ACCTGGCCTCTGACGAGCAGGACTCGGTGCGGCTGCTGGCGGTGGNAGCGTGCGTGAACA
  TCGCCCAGCTTCTGCCCCAGNAGGATCTGGAGGCCCTGGTGATGCCCACTCTGCGCCAGG
  CCGCTGAAGACAGTCCTGGCGCGTNCGCTACATGGTGGCCTGACAGNTCACAGAGCTCCA
  GAAAGCATGG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014225 unedited
  GGCCGCATTCTAGAGTCGAGTTTTTTTTTTTTTTTTTTACTTCTAAACCTTTATTTCTCT
  GTGAAAAGGGGGAAAATAAAAGGAATAAAATAAAAACGGCACAGTTGACACACAAAAAAA
  AACCAATGATGGGGAGGACGGGAGGTGGAGAAGTAAATGGGGGAGGGGTTCCCATTACAG
  CAGCAGGATCCAGTGACCCGGGATGCTCACATCTCTCCCTGACGTGGGCGGAGTAGCCCC
  TTCCTCCCAAGGTCACTGTCCTGTCCAACCCCGTGCTCCCCTAGCCCGTTGGGAGGTGGA
  CAGTGAGACATCTTCCCAGGCTGGGGAGAGGAGGAACCGTGCTGGGGGAAGGGGCCTGGG
  GTCAGACCATGCACAGGGAGTGACAGCCAAAGGCCCCCCAGTGTCTCCCCAAAGAGGGAC
  TTGTGGGGGTTGGAGGGTGGACACCAGAGGCCAGTGTTTGCTCCTCTTCCAGCATCAGGC
  GAGAGACAGAACAGTCAGAGCCTCCTGGGCAAAGTATTTGACGTCCACATCCTGGTCCTG
  GGTCAGCTTCTCTAGGATGGGCTTGACTTCCCTCTGCAAGGCGCTGTTGTCCACGATGGG
  CCCTATCTTCTGCAGAGACTTGGCCCCATTGAATCGGACATTGGCAACCGGGTCCCCAGC
  CATGCCCCTAACCGTGGGTACCCCGTGCTCGGTGGTGATGCCCCGCCCCATACACCTTAT
  ATAGTCCCTCGCCCCCCAAACACCGTTTCCTGCCCCCCCTGGTTCTCGGGTCTTTCTGAC
  ATGGCCCAAACCTTGCGGCTATTTCGTGTCTGGCCCCCCTCTTTCCCACCCCTCCCTGTC
  TTCCTAGGCCCCTGCCGCGTGCCTTCCGCCTGACCCACCCGCCCGAATCCCAGCCTTCCC
  CCGATCAACCTCTTCATATCCCCTTTCTCCCGTTTCACCCCAATCCTTGCCTCCCCCCCC
  CCCATCCAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014225
• Insert Size: 2240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014225.3, NP_055040.2
• RefSeq Size: 2357 bp
• RefSeq ORF: 1770 bp
• Locus ID: 5518
• UniProt ID: P30153
• Cytogenetics: 19q13.41
• Domains: HEAT, HEAT_PBS
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Protein Pathways: Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway
• Gene Summary: This gene encodes a constant regulatory subunit of protein phosphatase 2. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The constant regulatory subunit A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. This gene encodes an alpha isoform of the constant regulatory subunit A. Alternatively spliced transcript variants have been described. [provided by RefSeq, Apr 2010]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein.