Ku70 (XRCC6) (NM_001469) Human Untagged Clone

CAT#: SC110901

XRCC6 (untagged)-Human X-ray repair complementing defective repair in Chinese hamster cells 6 (XRCC6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Ku70
• Synonyms: CTC75; CTCBF; G22P1; KU70; ML8; TLAA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110901 sequence for NM_001469 edited (data generated by NextGen Sequencing)
  ATGTCAGGGTGGGAGTCATATTACAAAACCGAGGGCGATGAAGAAGCAGAGGAAGAACAA
  GAAGAGAACCTTGAAGCAAGTGGAGACTATAAATATTCAGGAAGAGATAGTTTGATTTTT
  TTGGTTGATGCCTCCAAGGCTATGTTTGAATCTCAGAGTGAAGATGAGTTGACACCTTTT
  GACATGAGCATCCAGTGTATCCAAAGTGTGTACATCAGTAAGATCATAAGCAGTGATCGA
  GATCTCTTGGCTGTGGTGTTCTATGGTACCGAGAAAGACAAAAATTCAGTGAATTTTAAA
  AATATTTACGTCTTACAGGAGCTGGATAATCCAGGTGCAAAACGAATTCTAGAGCTTGAC
  CAGTTTAAGGGGCAGCAGGGACAAAAACGTTTCCAAGACATGATGGGCCACGGATCTGAC
  TACTCACTCAGTGAAGTGCTGTGGGTCTGTGCCAACCTCTTTAGTGATGTCCAATTCAAG
  ATGAGTCATAAGAGGATCATGCTGTTCACCAATGAAGACAACCCCCATGGCAATGACAGT
  GCCAAAGCCAGCCGGGCCAGGACCAAAGCCGGTGATCTCCGAGATACAGGCATCTTCCTT
  GACTTGATGCACCTGAAGAAACCTGGGGGCTTTGACATATCCTTGTTCTACAGAGATATC
  ATCAGCATAGCAGAGGATGAGGACCTCAGGGTTCACTTTGAGGAATCCAGCAAGCTAGAA
  GACCTGTTGCGGAAGGTTCGCGCCAAGGAGACCAGGAAGCGAGCACTCAGCAGGTTAAAG
  CTGAAGCTCAACAAAGATATAGTGATCTCTGTGGGCATTTATAATCTGGTCCAGAAGGCT
  CTCAAGCCTCCTCCAATAAAGCTCTATCGGGAAACAAATGAACCAGTGAAAACCAAGACC
  CGGACCTTTAATACAAGTACAGGCGGTTTGCTTCTGCCTAGCGATACCAAGAGGTCTCAG
  ATCTATGGGAGTCGTCAGATTATACTGGAGAAAGAGGAAACAGAAGAGCTAAAACGGTTT
  GATGATCCAGGTTTGATGCTCATGGGTTTCAAGCCGTTGGTACTGCTGAAGAAACACCAT
  TACCTGAGGCCCTCCCTGTTCGTGTACCCAGAGGAGTCGCTGGTGATTGGGAGCTCAACC
  CTGTTCAGTGCTCTGCTCATCAAGTGTCTGGAGAAGGAGGTTGCAGCATTGTGCAGATAC
  ACACCCCGCAGGAACATCCCTCCTTATTTTGTGGCTTTGGTGCCACAGGAAGAAGAGTTG
  GATGACCAGAAAATTCAGGTGACTCCTCCAGGCTTCCAGCTGGTCTTTTTACCCTTTGCT
  GATGATAAAAGGAAGATGCCCTTTACTGAAAAAATCATGGCAACTCCAGAGCAGGTGGGC
  AAGATGAAGGCTATCGTTGAGAAGCTTCGCTTCACATACAGAAGTGACAGCTTTGAGAAC
  CCCGTGCTGCAGCAGCACTTCAGGAACCTGGAGGCCTTGGCCTTGGATTTGATGGAGCCG
  GAACAAGCAGTGGACCTGACATTGCCCAAGGTTGAAGCAATGAATAAAAGACTGGGCTCC
  TTGGTGGATGAGTTTAAGGAGCTTGTTTACCCACCAGATTACAATCCTGAAGGGAAAGTT
  ACCAAGAGAAAACACGATAATGAAGGTTCTGGAAGCAAAAGGCCCAAGGTGGAGTATTCA
  GAAGAGGAGCTGAAGACCCACATCAGCAAGGGTACGCTGGGCAAGTTCACTGTGCCCATG
  CTGAAAGAGGCCTGCCGGGCTTACGGGCTGAAGAGTGGGCTGAAGAAGCAGGAGCTGCTG
  GAAGCCCTCACCAAGCACTTCCAGGACTGA
  
  Clone variation with respect to NM_001469.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001469 unedited
  TTTGTAATACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGGTTGGTCGCTTCCC
  TGCGCCAAAGTGAGCAGTAGCCAACATGTCAGGGTGGGAGTCATATTACAAAACCGAGGG
  CGATGAAGAAGCAGAGGAAGAACAAGAAGAGAACCTTGAAGCAAGTGGAGACTATAAATA
  TTCAGGAAGAGATAGTTTGATTTTTTTGGTTGATGCCTCCAAGGCTATGTTTGAATCTCA
  GAGTGAAGATGAGTTGACACCTTTTGACATGAGCATCCAGTGTATCCAAAGTGTGTACAT
  CAGTAAGATCATAAGCAGTGATCGAGATCTCTTGGCTGTGGTGTTCTATGGTACCGAGAA
  AGACAAAAATTCAGTGAATTTTAAAAATATTTACGTCTTACAGGAGCTGGATAATCCAGG
  TGCAAAACGAATTCTAGAGCTTGACCAGTTTAAGGGGCAGCAGGGACAAAAACGTTTCCA
  AGACATGATGGGCCACGGATCTGACTACTCACTCAGTGAAGTGCTGTGGGTCTGTGCCAA
  CCTCTTTAGTGATGTCCAATTCAAGATGAGTCATAAGAGGATCATGCTGTTCACCAATGA
  AGACAACCCCCATGGCAATGACAGTGCCAAAGCCAGCCGGGCCAGGACCAAAGCCGGTGA
  TCTCCGAGATACAGGCATCTTCCTTGACTTGATGCACCTGAAGAAACCTGGGGGCTNTGA
  CATATNNCTTGNTCTACAGAGATATCATCAGCATAGCAGAGGATGANGGACCTCAGGGNT
  TCACTTTNGAGAAATCCAGCAGCTANAAGACCTGTTGCCGGAAGGGTCGCGCCAAGGAGA
  CCCAGAAGCGAGCACTCAGCAGGTTAAAGCTGAAGCTCACAAAGATTTATGATCTCTTGG
  GCATTTATATC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001469 unedited
  CTATGGACCGGCGGCCGCAATCTAGGATCGAGTTTTTTTTTTTTTTTTTATAGTACAAAC
  TTAGGGCTCTTTATTCAGGCAGTAAAGTAAGGAACAGCAAAGTGGGAGGGCTACACCATC
  ACCATGGCAACAGAAAGCCTCAAAAACATAAAGTCCCTCGACTTATGTCGGGTAGACTCT
  TCCTAGCTCAGGAGAAACACATTTTAACTGGCTGAGGACAAGGCCAGGCAGCCTGGCCAC
  ACTGCGGAAGGGCAGCTGGACGCGCGGCCTCTGGTCAGTCCTGGAAGTGCTTGGTGAGGG
  CTTCCAGCAGCTCCTGCTTCTTCAGCCCACTCTTCAGCCCGTAAGCCCGGCAGGCCTCTT
  TCAGCATGGGCACAGTGAACTTGCCCAGCGTACCCTTGCTGATGTGGGTCTTCAGCTCCT
  CTTCTGAATACTCCACCTTGGGCCTTTTGCTTCCAGAACCTTCATTATCGTGTTTTCTCT
  TGGTAACTTTCCCTTCAGGATTGTAATCTGGTGGGTAAACAAGCTCCTTAAACTCATCCA
  CCAAGGAGCCCAGTCTTTTATTCATTGCTTCAACCTTGGGCAATGTCAGGTCCACTGCTT
  GTTCCGGCTCCATCAAATCCAAGGCCAAGGCCTNCAGGTTCCTGAAGTGCTGCTGCAGCA
  CGGNGTTCTCANAGCTGTCACTTCTGTATGTGAAGCGAAGCTTCTCAACGATAGCCTTCA
  TCTTGCCCACCTGCGTCTGGAGTGCCATGATTNTTTCAGTAAAGGGCATCTTCCTTTTAT
  CATCAGCAAAGGGTAAAAAGACCAGCTGGAAGCCTGGAGGAGTCACCTNNGAATTTCTGG
  TCATCCAACTCTTNCTTCTGTGGGCACCAAAGCCACAATAAGGAGGGATGTNCCTGCGGG
  TGTGTATCTGCACATGCTGCACCCTCTTTNCAGACACTGAGAGCANACACTGNACAGGTT
  GAGCNCCCATACAGC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001469
• Insert Size: 2240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001469.3, NP_001460.1
• RefSeq Size: 2156 bp
• RefSeq ORF: 1830 bp
• Locus ID: 2547
• UniProt ID: P12956
• Cytogenetics: 22q13.2
• Domains: SAP, Ku_C, Ku_N, ku
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Non-homologous end-joining
• Gene Summary: The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions as a single-stranded DNA-dependent ATP-dependent helicase. The complex may be involved in the repair of nonhomologous DNA ends such as that required for double-strand break repair, transposition, and V(D)J recombination. High levels of autoantibodies to p70 and p80 have been found in some patients with systemic lupus erythematosus. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest protein (isoform 1). Variants 1 and 2 encode the same protein.