EXOSC7 (NM_015004) Human Untagged Clone

CAT#: SC101005

EXOSC7 (untagged)-Human exosome component 7 (EXOSC7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EXOSC7
• Synonyms: EAP1; hRrp42p; p8; RRP42; Rrp42p
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC101005 sequence for NM_015004 edited (data generated by NextGen Sequencing)
  ATGGCGTCCGTGACGCTGAGCGAGGCGGAGAAGGTGTACATCGTGCATGGCGTCCAGGAA
  GACCTCCGTGTGGATGGCCGTGGCTGTGAGGACTACCGATGTGTCGAAGTGGAAACTGAT
  GTGGTGTCCAACACTAGTGGGTCCGCCAGGGTCAAGCTGGGTCACACAGACATCTTGGTG
  GGAGTGAAAGCAGAAATGGGGACGCCGAAGCTGGAGAAACCAAATGAAGGCTACTTGGAG
  TTCTTTGTTGATTGTTCAGCCAGTGCTACCCCTGAATTTGAAGGTAGAGGAGGTGATGAC
  CTTGGCACCGAGATCGCTAACACCCTCTATCGGATATTTAACAATAAAAGCAGTGTCGAC
  TTAAAGACCCTCTGCATTAGTCCTCGGGAGCACTGCTGGGTTCTCTATGTGGATGTGCTG
  CTTCTGGAATGTGGTGGAAATTTGTTTGATGCCATTTCCATTGCTGTAAAGGCTGCTCTC
  TTCAATACAAGGATACCAAGGGTTCGAGTTTTGGAGGATGAAGAGGGGTCGAAGGACATT
  GAATTGTCAGATGACCCTTATGACTGCATACGACTAAGTGTGGAGAATGTCCCCTGCATT
  GTCACTCTGTGCAAGATTGGCTATCGGCATGTGGTGGATGCTACTCTTCAGGAGGAGGCC
  TGCTCGCTGGCCAGCTTGCTGGTGTCGGTGACCAGCAAGGGAGTTGTGACGTGCATGAGG
  AAAGTGGGGAAGGGCAGCCTGGACCCAGAGAGCATCTTCGAGATGATGGAGACTGGCAAG
  CGTGTGGGCAAGGTACTGCATGCCTCCTTGCAGAGTGTTGTGCACAAGGAAGAAAGCCTG
  GGGCCCAAGAGACAGAAAGTTGGATTCCTGGGATGA
  
  Clone variation with respect to NM_015004.3
  252 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015004 unedited
  CCTTCCGCCGCCCGTTGCCGCTTATGGGCGTAGGCGTGTACGGTGGGAGGTCTATATAAG
  CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC
  GAATTCGGCACGAGGGGGGCAGCTCGGCAGCATGGCGTCCGTGACGCTGAGCGAGGCGGA
  GAAGGTGTACATCGTGCATGGCGTCCAGGAAGACCTCCGTGTGGATGGCCGTGGCTGTGA
  GGACTACCGATGTGTCGAAGTGGAAACTGATGTGGTGTCCAACACTAGTGGGTCCGCCAG
  GGTCAAGCTGGGTCACACAGACATCTTGGTGGGAGTGAAAGCAGAAATGGGGACGCCGAA
  GCTGGAGAAACCAAATGAAGGCTACTTGGAGTTCTTTGTTGATTGTTCAGCCAGTGCTAC
  CCCTGAATTTGAAGGTAGAGGAGGTGATGACCTTGGCACCGAGATCGCTAACACCCTCTA
  TCGGATATTTAACAATAAAAGCAGTGTCGACTTAAAGACCCTCTGCATTAGTCCTCGGGA
  GCACTGCTGGGTTCTCTATGTGGATGTGCTGCTTCTGGAATGTGGTGGAAATTTGTTTGA
  TGCCATTTCCATTGCTGTAAAGGCTGCTCTCTTCATACAAGGATACCAAGGGTTCGAGTT
  TTGGAGGATGAAGAGGGGTCGAAGGACATTGAATTGTCAGATGACCTTNATGACTGCATA
  CGACTAAGTGTGGAGAATGTCCCCTGCATTGTCACTCTGTGCAAGATTGGGCTATCGCAT
  GTGGTGGATGCTACTCTTTCAGAGGAGCCCTGCTCGCTGGCCAGCTTGCTGGTGTCCGTG
  ACCAGCAAGGGAGATGTGACCTGCATGANGAAAGTGGGGAAAGGCAGCCTGGACCCGAGA
  GCACTTTCNATATAATGAGACTGGCAAGCTGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015004 unedited
  GTCGCTACCCCNAATCAGAGTTGAGTTTTAAATCTTTTTTTTTTTTTTTTTTTTTTTTCC
  AGACCAAAAAATAGCCTTTAATTTTACATGTACAAATGCTGCTGTAAATTCGTAACAGCG
  AAAAAAAATATATACGAACCGGTTTAAAAGGAAAAGTAAAAAACAATCCACAGTTGAGCA
  GTTGATGTGCAAATCATCCCAGGAATCCAACTTTCTGTCTCTTGGGCCCCAGGCTTTCTT
  CCTTGTGCACAACACTCTGCAAGGAGGCATGCAGTACCTTGCCCACACGCTTGCCAGTCT
  CCATCATCTCGAAGATGCTCTCTGGGTCCAGGCTGCCCTTCCCCACTTTCCTCATGCACG
  TCACAACTCCCTTGCTGGTCACCGACACCAGCAAGCTGGCCAGCGAGCAGGCCTCCTCCT
  GAAGAGTAGCATCCACCACATGCCGATAGCCAATCTTGCACAGAGTGACAATGCAGGGGA
  CATTCTCCACACTTAGTCGTATGCAGTCATAAGGGTCATCTGACAATTCAATGTCCTTCG
  ACCCCTCTTCATCCTCCAAAACTCGAACCCTTGGTATCCTTGTATTGAAGAGAGCAGCCT
  TTACAGCAATGGAAATGGCATCAAACAAATTTCCACCACATTCCAGAAGCAGCACATCCA
  CATAGAGAACCCAGCAGTGCTCCCGAGGACTAATGCAGAGGGGTCTTAAGTCGACACTGC
  TTTTATTGTTAAATATCCGATANGAGGGTGGTAGCGATCTNCGTGCCAAGGTCATCACCT
  CCTCTACCTTCAAATTCAGGGGTAGCACTGGCTGANNNCATCACAAGGACTCCNAGTAGC
  CCTTCATTGGTTTCTTCAGCTTCGGGGTCCCCATTTTGGCTTTATTCCACCAAGATGTCT
  GGGTGACCAAGTTTGCCNTGGCGAACCCCTATGGTTGAAACCAAATCAGTTTCACTTCGA
  ACATGGGTAGCCTCCACGCCACGCCTTCCN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_015004
• Insert Size: 1200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015004.2, NP_055819.1
• RefSeq Size: 1036 bp
• RefSeq ORF: 876 bp
• Locus ID: 23016
• UniProt ID: Q15024
• Cytogenetics: 3p21.31
• Domains: RNase_PH_C
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: RNA degradation
• Gene Summary: Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. The catalytic inactive RNA exosome core complex of 9 subunits (Exo-9) is proposed to play a pivotal role in the binding and presentation of RNA for ribonucleolysis, and to serve as a scaffold for the association with catalytic subunits and accessory proteins or complexes.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the protein.